Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,781,768 |
T→C |
34.4% |
F73F (TTT→TTC) |
yfjD → |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,781,768 | 0 | T | C | 34.4%
| 61.1
/ 49.2
| 87 | F73F (TTT→TTC) | yfjD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (36/21); new base C (4/26); total (40/47) |
| Fisher's exact test for biased strand distribution p-value = 1.01e-05 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCT > minE/1781706‑1781834
|
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGTCTTTTGTCGt > 1:3410559/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTc > 1:3086236/1‑66 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCg > 1:2747033/1‑67 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2055274/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2098523/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2371700/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2374221/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:239761/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:1560504/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2786024/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:3237664/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:1976946/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:653992/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:730417/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:773059/1‑68 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1883298/1‑69 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1921077/1‑69 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:1895713/69‑1 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:1051496/69‑1 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:2134023/1‑69 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:2319367/69‑1 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:3359722/1‑69 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:42187/1‑69 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:489925/69‑1 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:493486/69‑1 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:887564/1‑69 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:1417593/69‑1 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1386945/1‑69 (MQ=255)
aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1347952/1‑69 (MQ=255)
attttgGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAATGCGACTGGTGTGCTGACTTTTGTCGTAc > 1:1753910/4‑69 (MQ=255)
tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:185610/1‑69 (MQ=255)
tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:3132694/1‑69 (MQ=255)
tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:1592228/1‑69 (MQ=255)
tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:2217939/1‑69 (MQ=255)
tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:2201517/1‑69 (MQ=255)
tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:1259206/69‑1 (MQ=255)
tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:3331871/69‑1 (MQ=255)
tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:382211/69‑1 (MQ=255)
tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:2016217/69‑1 (MQ=255)
tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:535957/69‑1 (MQ=255)
tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:1740080/69‑1 (MQ=255)
tGTACGGCGATGCGGGCGTGGCAATTGAGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:2555262/69‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1002544/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2940175/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:980345/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1462760/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:3272812/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:3303441/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:926919/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:3373867/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1051553/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:48408/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1171893/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1816740/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:708917/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:705083/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2912985/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2077059/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1727498/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:170288/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2324799/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1569957/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2729237/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1548315/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2759978/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2796098/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2796797/66‑1 (MQ=255)
atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1543873/66‑1 (MQ=255)
cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt < 1:3166493/68‑1 (MQ=255)
cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt < 1:2908020/68‑1 (MQ=255)
ggCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:458224/68‑1 (MQ=255)
ggCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:1895410/68‑1 (MQ=255)
ggCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:2189055/68‑1 (MQ=255)
ggCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt < 1:70601/53‑1 (MQ=255)
ggCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTATTGCCGAAAACCATTGc > 1:3147088/1‑69 (MQ=255)
ggCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTATTGCCGAAAACCATTGc > 1:1980711/1‑69 (MQ=255)
ggCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTATTGCCGAAAACCATTGc > 1:140396/1‑69 (MQ=255)
ggCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTATTGCCGAAAACCATTGc > 1:1728477/1‑69 (MQ=255)
gCGACTGGTGTGCTGACTTTTGTCTTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTg > 1:1721356/1‑69 (MQ=255)
gCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTg > 1:1620782/1‑69 (MQ=255)
tGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:3401566/51‑1 (MQ=255)
tgCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:790615/46‑1 (MQ=255)
cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTa > 1:177341/1‑59 (MQ=255)
cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaaaaa > 1:918640/1‑69 (MQ=255)
cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaaaaa > 1:2975779/1‑69 (MQ=255)
cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaaaaa > 1:3386557/1‑69 (MQ=255)
cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaaaaa > 1:1465614/1‑69 (MQ=255)
ttttGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg < 1:1197884/68‑1 (MQ=255)
ttttGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg < 1:2703448/68‑1 (MQ=255)
ttttGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg < 1:370971/68‑1 (MQ=255)
tttGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCt > 1:3085011/1‑69 (MQ=255)
|
ATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCT > minE/1781706‑1781834
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
