Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,845,301 |
C→T |
56.3% |
D232N (GAC→AAC) |
cysD ← |
sulfate adenylyltransferase, subunit 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,845,301 | 0 | C | T | 56.3%
| 18.1
/ 69.2
| 87 | D232N (GAC→AAC) | cysD | sulfate adenylyltransferase, subunit 2 |
| Reads supporting (aligned to +/- strand): ref base C (9/29); new base T (42/7); total (51/36) |
| Fisher's exact test for biased strand distribution p-value = 4.34e-09 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 112 total aligned reads displayed.
CAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGA > minE/1845233‑1845368
|
cAGCGGCCAGCTGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTc < 1:2099748/69‑1 (MQ=255)
cAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTc < 1:388127/69‑1 (MQ=255)
cAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTc > 1:1125582/1‑69 (MQ=255)
cAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTc < 1:2896096/69‑1 (MQ=255)
cAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTc > 1:466569/1‑69 (MQ=255)
cAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTc < 1:1392534/69‑1 (MQ=255)
cAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTc < 1:2103721/69‑1 (MQ=255)
cAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTc < 1:2703886/69‑1 (MQ=255)
cAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTc < 1:3113942/69‑1 (MQ=255)
gTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCatcaatc > 1:377943/1‑69 (MQ=255)
gTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCatcaatc > 1:122899/1‑69 (MQ=255)
gTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCatcaat > 1:521187/1‑68 (MQ=255)
gAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAatcatcat < 1:975768/69‑1 (MQ=255)
gAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAatcatcat < 1:223156/69‑1 (MQ=255)
aCGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCaa < 1:2691315/69‑1 (MQ=255)
cATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAatcatcat < 1:1132807/60‑1 (MQ=255)
cGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTcg > 1:1602721/1‑69 (MQ=255)
cGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTcg > 1:897425/1‑69 (MQ=255)
cGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTcg > 1:2704760/1‑69 (MQ=255)
cGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTcg > 1:2858576/1‑69 (MQ=255)
ttttttAATCACTTCGCCCGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTcgcg < 1:2895425/69‑1 (MQ=255)
ttttttAATCACTTCGCCCGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTcgcg < 1:749442/69‑1 (MQ=255)
ttttttAATCACTTCGCCCGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTcgcg < 1:19629/69‑1 (MQ=255)
ttttttAATCACTTCGCCCGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTcgcg < 1:2088270/69‑1 (MQ=255)
ttttttAATCACTTCGCCCGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTcgcg < 1:2886547/69‑1 (MQ=255)
tttttAATCACTTCGCCCGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTcgcg < 1:3100896/68‑1 (MQ=255)
tCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGa < 1:858823/68‑1 (MQ=255)
tCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGa < 1:388012/68‑1 (MQ=255)
tCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGa < 1:3202641/68‑1 (MQ=255)
cACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGa < 1:1289153/67‑1 (MQ=255)
cGCCCGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTcgcg < 1:2146372/55‑1 (MQ=255)
cccGCTTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:1335153/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGAc > 1:1788984/1‑67 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:2310490/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:2411541/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:553793/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:2615273/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:2858479/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:220109/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:635553/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:1965156/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:833126/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:846047/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:1521937/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:1339143/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:987563/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:1264233/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:120868/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACg > 1:101849/1‑68 (MQ=255)
cccGATTGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTACAGAACCGGACg > 1:1519777/1‑68 (MQ=255)
ggTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTc > 1:793767/1‑68 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGtt > 1:2774254/3‑52 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTc > 1:2548974/3‑53 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:990389/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:2072251/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:2630485/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:578148/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:2297671/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:1972634/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:3428392/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:1866992/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:1659684/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:1709409/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGtt > 1:3391723/3‑67 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGt > 1:3228848/3‑66 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTCGCGTTCCAGAACCGGACGTTc > 1:432895/3‑68 (MQ=255)
gattGCAGGTTGATACGGTTGTCATCAATCATCATCAGCATACCGTAGCGTTCCAGAACCGGAc > 1:2370415/3‑64 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:1816165/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:841110/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:1911265/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:864866/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:878464/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:886641/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:1408448/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:1336236/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:447119/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:1010045/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:2814777/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:274074/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:294564/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:314461/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:2295882/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:373128/69‑1 (MQ=255)
ggTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCga < 1:3404124/69‑1 (MQ=255)
gTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgag < 1:3161041/69‑1 (MQ=255)
tCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga < 1:3202175/69‑1 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGCACCGGACGTTCCGCAGCgaga > 1:733206/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACg < 1:79632/55‑1 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACg < 1:3393690/55‑1 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACg < 1:2142513/55‑1 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga > 1:414398/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga > 1:2302711/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga > 1:2002357/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga > 1:2295556/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga > 1:2439147/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga > 1:3042495/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga > 1:2963415/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga > 1:409437/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgaga > 1:412582/1‑68 (MQ=255)
cGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCgag > 1:1297572/1‑67 (MQ=255)
|
CAGCGGCCAGCAGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTCATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGA > minE/1845233‑1845368
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
