Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,847,097 |
G→C |
24.2% |
G284G (GGG→GGC) |
iap → |
aminopeptidase in alkaline phosphatase isozyme conversion |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,847,097 | 0 | G | C | 24.2%
| 148.9
/ 45.7
| 93 | G284G (GGG→GGC) | iap | aminopeptidase in alkaline phosphatase isozyme conversion |
| Reads supporting (aligned to +/- strand): ref base G (46/24); new base C (17/5); total (64/29) |
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
GACGCAGAAATATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATG > minE/1847029‑1847161
|
gACGCAGAAATATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTggg < 1:2303369/69‑1 (MQ=255)
gACGCAGAAATATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTggg < 1:2326197/69‑1 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTggg > 1:2508298/1‑61 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa < 1:664246/69‑1 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa > 1:1490725/1‑69 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa > 1:2735002/1‑69 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa > 1:1309765/1‑69 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa < 1:3191119/69‑1 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa > 1:3158885/1‑69 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa > 1:2891536/1‑69 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa > 1:880349/1‑69 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa < 1:2976031/69‑1 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa < 1:964497/69‑1 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa < 1:2937816/69‑1 (MQ=255)
aaTATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa > 1:2625187/1‑69 (MQ=255)
aaTATTCGACAAAGCGGCCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAgta > 1:2788615/1‑67 (MQ=255)
aaTATTAGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGa > 1:435768/1‑69 (MQ=255)
ttCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGt < 1:2787652/69‑1 (MQ=255)
ttCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGt < 1:1020807/69‑1 (MQ=255)
tGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa < 1:764097/69‑1 (MQ=255)
tGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa < 1:59053/69‑1 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAg < 1:2696747/45‑1 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAg < 1:1923686/45‑1 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAg < 1:1889212/45‑1 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAg < 1:2049563/45‑1 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAg < 1:2177948/45‑1 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAg < 1:433229/45‑1 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAg < 1:390882/45‑1 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAg < 1:1804345/45‑1 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa > 1:408165/1‑68 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa > 1:2923471/1‑68 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa > 1:2596339/1‑68 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa > 1:2804015/1‑68 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa > 1:546189/1‑68 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa > 1:1577381/1‑68 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa > 1:1801056/1‑68 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa > 1:49146/1‑68 (MQ=255)
gCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCaaa > 1:511328/1‑68 (MQ=255)
tACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCGACGCGCAAAAacac > 1:1834892/1‑69 (MQ=255)
tACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAacac > 1:1334256/1‑69 (MQ=255)
tACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAacac > 1:2862906/1‑69 (MQ=255)
tACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAacac > 1:2992193/1‑69 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCaa > 1:165401/1‑35 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:473367/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:2687683/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:2978215/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:1013365/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:3142062/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:981266/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:1166899/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:1220322/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:1265141/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:1306806/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:1541559/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:1859064/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:3173617/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacagc > 1:301212/1‑66 (MQ=255)
cTTTCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacag > 1:172988/1‑66 (MQ=255)
atttCGGTGGAAGCGACTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAacag > 1:2401904/2‑66 (MQ=255)
ttCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGc > 1:869376/1‑69 (MQ=255)
ggAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTccc > 1:1133200/1‑69 (MQ=255)
ggAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTccc > 1:998719/1‑69 (MQ=255)
aCTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGa < 1:85623/69‑1 (MQ=255)
aCTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGa < 1:2624990/69‑1 (MQ=255)
cTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGa < 1:2385756/68‑1 (MQ=255)
cTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGa < 1:1353011/68‑1 (MQ=255)
cTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGa < 1:3414888/68‑1 (MQ=255)
cTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAACAGCCGCATTccc < 1:2901969/61‑1 (MQ=255)
cTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAACAGCCGCATTccc < 1:891852/61‑1 (MQ=255)
cTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAACAGCCGCATTccc < 1:1135598/61‑1 (MQ=255)
cTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAACAGCCGCATTccc < 1:1260132/61‑1 (MQ=255)
cTAACTGGAATCTTGGCAATAAGGATGGTTATCAGCAACGCGCAAAAACAGCCGCATTccc < 1:2153827/61‑1 (MQ=255)
aaCTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAAt < 1:3424269/69‑1 (MQ=255)
gAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCt > 1:1057058/1‑68 (MQ=255)
gAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCt > 1:1275830/1‑68 (MQ=255)
gAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCt > 1:1480864/1‑68 (MQ=255)
gAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCt > 1:546355/1‑68 (MQ=255)
gAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCt > 1:1572346/1‑68 (MQ=255)
aTCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTgg < 1:2816045/68‑1 (MQ=255)
ttGGTAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:1901695/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCaaaaa > 1:3406616/1‑36 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAaca > 1:2216808/1‑38 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAaca > 1:2229212/1‑38 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAaca > 1:1551938/1‑38 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAaca > 1:669323/1‑38 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAaca > 1:237949/1‑38 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCTGCGGGAAATAGCTGGc > 1:961398/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:1931192/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:1019121/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:2579758/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:1104819/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:2465367/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:3060521/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:2441115/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:2320801/1‑66 (MQ=255)
ttGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGc > 1:2917167/1‑66 (MQ=255)
gggAATAAGGATGGTTATCAACAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCAt > 1:1890293/1‑66 (MQ=255)
gAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATg < 1:2399258/65‑1 (MQ=255)
gAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATg < 1:699817/65‑1 (MQ=255)
gAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATg < 1:538321/65‑1 (MQ=255)
|
GACGCAGAAATATTCGACAAAGCGGGCATTGCTGTACTTTCGGTGGAAGCGACTAACTGGAATCTTGGGAATAAGGATGGTTATCAGCAACGCGCAAAAACACCTGCCTTCCCGGCGGGAAATAGCTGGCATG > minE/1847029‑1847161
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
