Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,857,716 |
C→G |
29.1% |
E27Q (GAA→CAA) |
cysH ← |
3'‑phosphoadenosine 5'‑phosphosulfate reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,857,716 | 0 | C | G | 29.1%
| 101.0
/ 48.6
| 79 | E27Q (GAA→CAA) | cysH | 3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Reads supporting (aligned to +/- strand): ref base C (35/21); new base G (12/11); total (47/32) |
| Fisher's exact test for biased strand distribution p-value = 4.54e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01 |
ACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAGGGCG > minE/1857649‑1857774
|
aCATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCg > 1:3123947/1‑69 (MQ=255)
aCATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCg > 1:2484376/1‑69 (MQ=255)
atTCACCGGGCAGTTTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:1025312/69‑1 (MQ=255)
atTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCTGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:2353284/69‑1 (MQ=255)
atTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:1527963/69‑1 (MQ=255)
atTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:1795892/69‑1 (MQ=255)
atTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:1889294/69‑1 (MQ=255)
atTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:2049122/69‑1 (MQ=255)
atTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:113925/69‑1 (MQ=255)
atTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:2525620/69‑1 (MQ=255)
atTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:843859/69‑1 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTGGGCGt < 1:2224393/68‑1 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGtt > 1:2289534/1‑69 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGtt > 1:265941/1‑69 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGtt > 1:37392/1‑69 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGtt > 1:2856797/1‑69 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGtt > 1:2567707/1‑69 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGtt > 1:145066/1‑69 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGtt > 1:1127491/1‑69 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGtt > 1:1290357/1‑69 (MQ=255)
ttCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGtt > 1:1561430/1‑69 (MQ=255)
cACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAg > 1:1632841/1‑69 (MQ=255)
cACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAg > 1:2965364/1‑69 (MQ=255)
cACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAg > 1:737889/1‑69 (MQ=255)
cACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAg > 1:3068065/1‑69 (MQ=255)
cACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAg > 1:1824385/1‑69 (MQ=255)
cACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAg > 1:337245/1‑69 (MQ=255)
cACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAg > 1:2212220/1‑69 (MQ=255)
cACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAg > 1:407680/1‑69 (MQ=255)
cACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAg > 1:2314132/1‑69 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag < 1:3311122/69‑1 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag < 1:3127893/69‑1 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag < 1:2349952/69‑1 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag < 1:1202903/69‑1 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag < 1:3060980/69‑1 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag < 1:1398953/69‑1 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag < 1:660157/69‑1 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag < 1:578889/69‑1 (MQ=255)
cAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCcgccagcgccag < 1:588846/69‑1 (MQ=255)
ccAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCg < 1:510731/69‑1 (MQ=255)
ccAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCg < 1:1906247/69‑1 (MQ=255)
ccAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCg < 1:1258612/69‑1 (MQ=255)
gCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:1274148/69‑1 (MQ=255)
gCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:1118871/69‑1 (MQ=255)
cTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:2581264/68‑1 (MQ=255)
cTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:2604295/68‑1 (MQ=255)
cTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:2705967/68‑1 (MQ=255)
cTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:2712962/68‑1 (MQ=255)
cTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:573379/68‑1 (MQ=255)
cTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:2994847/68‑1 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCTCCAGAATCCGGTCTACCttt > 1:1060952/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:978959/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:3391108/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:628852/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:3286394/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:1004973/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:2544470/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:1493338/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:1687885/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:1780323/1‑65 (MQ=255)
ccTTCAGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCtt > 1:765543/1‑65 (MQ=255)
ccTTCAGCGTCCAGCTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATCCGGTCTACCttt > 1:206733/1‑65 (MQ=255)
tCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:2834175/57‑1 (MQ=255)
aGCGTCCAGTTTTTCCAGTTGGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCt < 1:3099248/55‑1 (MQ=255)
cGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAgttcg > 1:902312/1‑68 (MQ=255)
cGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAgttcg > 1:3041478/1‑68 (MQ=255)
cGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAgttcg > 1:2794481/1‑68 (MQ=255)
cGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAgttcg > 1:3425607/1‑68 (MQ=255)
cAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCa > 1:3099234/1‑68 (MQ=255)
cAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCa > 1:1073496/1‑68 (MQ=255)
cAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCa > 1:345721/1‑68 (MQ=255)
cAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAg > 1:1698183/1‑69 (MQ=255)
cAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAg > 1:1795447/1‑69 (MQ=255)
cAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAg > 1:453488/1‑69 (MQ=255)
cAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAg > 1:770155/1‑69 (MQ=255)
cAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAg > 1:3097188/1‑69 (MQ=255)
cAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAg > 1:1007734/1‑69 (MQ=255)
ttttCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAggggg > 1:1080028/1‑67 (MQ=255)
ttttCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAGGGCg > 1:2338160/1‑69 (MQ=255)
|
ACATATTCACCGGGCAGATTATCCAGCGCCCAGGCTACGCGGCCTTCAGCGTCCAGTTTTTCCAGTTCGGCGTTAGTTTCCGCCAGCGCCAGAATGCGATCTACCTTCGGCAGTTCGTTCAGGGCG > minE/1857649‑1857774
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
