Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,872,697 |
T→C |
35.1% |
F440F (TTT→TTC) |
ygcE → |
predicted kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,872,697 | 0 | T | C | 35.1%
| 86.1
/ 76.9
| 128 | F440F (TTT→TTC) | ygcE | predicted kinase |
| Reads supporting (aligned to +/- strand): ref base T (43/40); new base C (32/13); total (75/53) |
| Fisher's exact test for biased strand distribution p-value = 3.99e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01 |
Only 100 of 133 total aligned reads displayed.
GGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCT > minE/1872632‑1872759
|
ggTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTtata > 1:908499/1‑69 (MQ=255)
ggTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTtata > 1:2231693/1‑69 (MQ=255)
ggTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTtata > 1:1947710/1‑69 (MQ=255)
ggTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTtata > 1:2515628/1‑69 (MQ=255)
tCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATAcc < 1:1783625/69‑1 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCg > 1:2233681/1‑68 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCg > 1:230725/1‑68 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:1663494/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:2553891/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:3238365/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:3326747/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:62038/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:3371099/1‑69 (MQ=255)
tGGGGCTATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:1335231/1‑69 (MQ=255)
tGGGGCTATACCCGCATTACACAACGGCTGTCGATAATATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:2431865/1‑69 (MQ=255)
tGGGGCGATACCCGGATTACACAACGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGa > 1:3020/1‑69 (MQ=255)
gggCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGa > 1:2714913/1‑67 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:3327540/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:388596/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:2139977/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:2761953/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:2844417/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:726139/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:1512264/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:1425078/67‑1 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:2826826/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:221201/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:1675949/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:1524093/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:268767/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:1360916/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:3381351/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:1481573/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:205960/1‑69 (MQ=255)
cccGGATTACGCAACGGCTGTCGATAACATGGATCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:2132856/1‑69 (MQ=255)
ccaggATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCaa > 1:2277287/4‑69 (MQ=255)
cacGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGc < 1:931381/65‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:200735/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:349137/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:1833466/69‑1 (MQ=255)
cGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATg < 1:1616353/69‑1 (MQ=255)
aCGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTac < 1:2418151/69‑1 (MQ=255)
aCGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTac < 1:267360/69‑1 (MQ=255)
cGGCTGTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTac < 1:312903/68‑1 (MQ=255)
ggCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTacga < 1:2289315/69‑1 (MQ=255)
ggCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTacga < 1:1059939/69‑1 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCt > 1:1707379/1‑61 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGActcg > 1:2638911/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:429752/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:429925/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:469108/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:1894122/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:243214/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:2509417/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:857445/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:2990893/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:1703275/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:3016647/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:1248946/1‑69 (MQ=255)
gTCGATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:632220/1‑69 (MQ=255)
gTCAATAAAATGGTTCGCGTGAAAGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGAcgcg > 1:122519/1‑69 (MQ=255)
aaCATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAt < 1:1151296/69‑1 (MQ=255)
aaCATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAt < 1:763810/69‑1 (MQ=255)
aaCATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAt < 1:1358426/69‑1 (MQ=255)
aaCATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAt < 1:1943788/69‑1 (MQ=255)
aaCATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAAt < 1:3074233/69‑1 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGAcgc < 1:1497708/56‑1 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGAcgc < 1:2132518/56‑1 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGAcgc < 1:1654844/56‑1 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGAcgc < 1:1796865/56‑1 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGAcgc < 1:1076624/56‑1 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGAt > 1:637478/1‑59 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGAt > 1:113316/1‑59 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGAt > 1:2033154/1‑59 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGAt > 1:3251220/1‑59 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGAt > 1:2979071/1‑59 (MQ=255)
gTTCGCGTGAAAGATATCTTTATACAGATTGAGAGCAATGCCAAACGCTACGACGCGAt > 1:2655439/1‑59 (MQ=255)
aGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAg < 1:853531/68‑1 (MQ=255)
aGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAg < 1:1315552/68‑1 (MQ=255)
aGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAg < 1:2643744/68‑1 (MQ=255)
aGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAg < 1:2655556/68‑1 (MQ=255)
aGATATCTTCATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAg < 1:1779973/68‑1 (MQ=255)
aGATATCTTCATACCGATTGAGAGCAATCCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAg < 1:1361286/68‑1 (MQ=255)
tatCTTTATCCCGATAGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:2998009/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:512466/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:1969878/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:1869415/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:176356/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:581070/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:1469878/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:1626504/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:464023/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:2852609/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:2861998/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:3372197/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:1339196/1‑69 (MQ=255)
tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCt > 1:1605689/1‑69 (MQ=255)
atCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAgg > 1:864019/1‑53 (MQ=255)
atCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAgg > 1:1047769/1‑53 (MQ=255)
atCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGAGATGAATAAAgg > 1:969113/1‑53 (MQ=255)
|
GGTCTGGGGCTATACCCGGATTACGCAACGGCTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCT > minE/1872632‑1872759
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
