Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,897,223 |
A→G |
29.4% |
P315P (CCA→CCG) |
argA → |
fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,897,223 | 0 | A | G | 29.4%
| 92.0
/ 43.5
| 95 | P315P (CCA→CCG) | argA | fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (26/41); new base G (13/15); total (39/56) |
| Fisher's exact test for biased strand distribution p-value = 5.03e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAA > minE/1897155‑1897286
|
gAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCa > 1:800519/1‑69 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:1479170/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:1461671/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:1401542/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2668550/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:1347279/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:1345687/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2763032/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:862214/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2206488/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2119929/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:3184359/69‑1 (MQ=255)
ccGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:818673/69‑1 (MQ=255)
cGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:91300/68‑1 (MQ=255)
cGAGCAGATTCGTCGCGCAACAATCAACGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2259412/68‑1 (MQ=255)
gAGCAGATTCGTCGCGCAACAATCATCGATATCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGagc < 1:2484970/67‑1 (MQ=255)
gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:1873488/69‑1 (MQ=255)
gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:73782/69‑1 (MQ=255)
gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:1245847/69‑1 (MQ=255)
gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:1200757/69‑1 (MQ=255)
gATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:351389/69‑1 (MQ=255)
gATTCGTCGCGCAACAATCAACGATATTGGCGGAATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg < 1:3142079/69‑1 (MQ=255)
ttCGTCGCGCAACAATCAAGGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGt > 1:2788910/1‑68 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGt > 1:2629138/1‑68 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa > 1:558509/1‑69 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa > 1:3378884/1‑69 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:824599/69‑1 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa > 1:3179443/1‑69 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:1767693/69‑1 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:1190648/69‑1 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:925718/69‑1 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:1854208/69‑1 (MQ=255)
ttCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa < 1:936442/69‑1 (MQ=255)
gcgcAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTgg < 1:1728433/69‑1 (MQ=255)
gcgcAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTgg < 1:167166/69‑1 (MQ=255)
gcgcAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTgg < 1:2739634/69‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1120087/60‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:2847593/60‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1221564/60‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1451846/60‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1453242/60‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:1466533/60‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:160171/60‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:3040000/60‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:849257/60‑1 (MQ=255)
aaTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCt < 1:3076280/60‑1 (MQ=255)
aTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGt < 1:2681626/69‑1 (MQ=255)
aTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGt < 1:1603264/69‑1 (MQ=255)
aTCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGt < 1:115095/69‑1 (MQ=255)
cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:3315917/1‑68 (MQ=255)
cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:2938241/1‑68 (MQ=255)
cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:3372361/1‑68 (MQ=255)
cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:275683/1‑68 (MQ=255)
cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:89444/1‑68 (MQ=255)
cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtctc > 1:2463467/1‑68 (MQ=255)
cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtct > 1:3368779/1‑67 (MQ=255)
cGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTtct > 1:1029741/1‑67 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGt > 1:1718001/1‑55 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:3376157/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:346360/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:675606/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:679556/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:793765/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2773093/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:3038475/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2076207/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2455724/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:1011712/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2790564/1‑69 (MQ=255)
atatCGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGTATTCTGGTGCGCCGTTCTcgcg > 1:2838834/1‑69 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGgag < 1:2858235/69‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGgag < 1:673468/69‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGgag < 1:2692554/69‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGga < 1:949178/68‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGga < 1:1065018/68‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGga < 1:2973233/68‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGga < 1:65387/68‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGga < 1:1667677/68‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGga < 1:2066903/68‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGga < 1:2150018/68‑1 (MQ=255)
aTTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTAGCGAGCAGCTGga < 1:553223/68‑1 (MQ=255)
ttCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGgaga > 1:1998028/1‑69 (MQ=255)
cTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGAt > 1:2835708/1‑68 (MQ=255)
cTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGAt > 1:1342446/1‑68 (MQ=255)
cTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGAt > 1:490910/1‑68 (MQ=255)
gATTCGCCCACTGGAGCAGCAAGGTATTCTGGTTCGCCGTTCTCGCGAGCAGCTGGAGATgg > 1:3153124/1‑62 (MQ=255)
gATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATgg > 1:790086/1‑62 (MQ=255)
gATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATgg > 1:2205898/1‑62 (MQ=255)
gATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATgg > 1:884348/1‑62 (MQ=255)
gATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATgg > 1:894222/1‑62 (MQ=255)
gATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATgg > 1:1398087/1‑62 (MQ=255)
gATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATgg > 1:1224956/1‑62 (MQ=255)
gATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATgg > 1:418447/1‑62 (MQ=255)
cGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACaaa > 1:2977410/1‑69 (MQ=255)
cGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACaaa < 1:15792/69‑1 (MQ=255)
cGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACaaa < 1:278471/69‑1 (MQ=255)
|
GAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAA > minE/1897155‑1897286
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
