Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,901,169 |
G→A |
56.4% |
N624N (AAC→AAT) |
recB ← |
exonuclease V (RecBCD complex), beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,901,169 | 0 | G | A | 56.4%
| 10.1
/ 40.9
| 55 | N624N (AAC→AAT) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (14/10); new base A (14/17); total (28/27) |
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTTCGACTACCACATCCCACGCATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACC > minE/1901111‑1901235
|
ctTCGACTACCGCATCCCACGCATGTTCGTCATTATTCAGCGTTTCAATATCCAGCGCATTCAGCCCca < 1:1152314/69‑1 (MQ=255)
ctTCGACTACCGCATCCCACGCATGTTCGTCATTATTCAGCGTTTCAATATCCAGCGCATTCAGCCCca < 1:848687/69‑1 (MQ=255)
ctTCGACTACCGCATCCCACGCATGTTCGTCATTATTCAGCGTTTCAATATCCAGCGCATTCAGCCCca < 1:1957973/69‑1 (MQ=255)
ctTCGACTACCGCATCCCACGCATGTTCGTCATTATTCAGCGTTTCAATATCCAGCGCATTCAGCCCca < 1:702369/69‑1 (MQ=255)
ctTCGACTACCGCATCCCACGCATGTTCGTCATTATTCAGCGTTTCAATATCCAGCGCATTCAGCCCca < 1:1280235/69‑1 (MQ=255)
ctTCGACTACCGCATCCCACGCATGTTCGTCATTATTCAGCGTTTCAATATCCAGCGCATTCAGCCCca < 1:582955/69‑1 (MQ=255)
ctTCGACTACCGCATCCCACGCATGTTCGTCATTATTCAGCGTTTCAATATCCAGCGCATTCAGCCCca < 1:2185511/69‑1 (MQ=255)
ctTCGACTACCGCATCCCACGCATGTTCGTCATTATTCAGCGTTTCAATATCCAGCGCATTCAGCCCca < 1:2321438/69‑1 (MQ=255)
ctTCGACTACCGCATCCCACGCATGTTCGTCATTATTCAGCGTTTCAATATCCAGCGCATTCAGCCCca < 1:2745888/69‑1 (MQ=255)
cACGCATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGa > 1:2622591/1‑59 (MQ=255)
cACGCATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGAc > 1:2476637/1‑60 (MQ=255)
cACGCATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGAc > 1:1642098/1‑60 (MQ=255)
cACGCATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGAc > 1:2115174/1‑60 (MQ=255)
cATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCTCGTTCAGCCCCATCATTGACGTTGCCAGCGCAc < 1:121856/69‑1 (MQ=255)
cATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCAc < 1:2547072/69‑1 (MQ=255)
cATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCAc < 1:2801361/69‑1 (MQ=255)
cATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCAc < 1:496769/69‑1 (MQ=255)
cATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCAc < 1:1853653/69‑1 (MQ=255)
aTGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCAc < 1:1621848/68‑1 (MQ=255)
aTGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCAc < 1:2023993/68‑1 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGc < 1:986201/40‑1 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGc < 1:154365/40‑1 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGc < 1:1627853/40‑1 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:2318095/1‑67 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:3020365/1‑67 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:305474/1‑67 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:92702/1‑67 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:3166948/1‑67 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:551093/1‑67 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:2050756/1‑67 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:2046787/1‑67 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:1814983/1‑67 (MQ=255)
ttCGTCATTGTTCAGCGTTTCGATATCCAGAGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTa > 1:2400026/1‑67 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:1404426/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:1324516/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:1207288/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:1275721/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:2967814/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:2907740/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:1513974/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:816600/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:1848835/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:886310/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:2565046/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:1555806/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:889485/3‑69 (MQ=255)
tattCAGCGTTTCAATATCCAGCGCATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGgtgt > 1:1659770/3‑69 (MQ=255)
gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:2739752/69‑1 (MQ=255)
gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:285945/69‑1 (MQ=255)
gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:3072191/69‑1 (MQ=255)
gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:1562450/69‑1 (MQ=255)
gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:487007/69‑1 (MQ=255)
gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:1965675/69‑1 (MQ=255)
gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca < 1:2116892/69‑1 (MQ=255)
gcgcATTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACATTCGGGTGtcatca < 1:651576/69‑1 (MQ=255)
cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCg > 1:186074/1‑39 (MQ=37)
cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGtcatca > 1:2847672/1‑68 (MQ=255)
cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:932365/1‑69 (MQ=255)
cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:2815581/1‑69 (MQ=255)
cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:1552229/1‑69 (MQ=255)
cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:2513352/1‑69 (MQ=255)
cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:1557313/1‑69 (MQ=255)
cgcaTTCAGCCCCATCATTGACGTTGCCAGCGCACTGCGCAGGGTGTTCTCACGTTCGGGTGTCATCAc > 1:2146854/1‑69 (MQ=255)
gcgTTCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCAcc < 1:1155636/69‑1 (MQ=255)
|
CTTCGACTACCACATCCCACGCATGTTCGTCATTGTTCAGCGTTTCGATATCCAGCGCGTTCAGCCCCATCATTGACGTTGCCAGCGCACTACGCAGGGTGTTCTCACGTTCGGGCGTCATCACC > minE/1901111‑1901235
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
