Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,910,142 |
C→T |
58.9% |
V18I (GTT→ATT) |
ygdB ← |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,910,142 | 0 | C | T | 58.9%
| 24.7
/ 52.1
| 73 | V18I (GTT→ATT) | ygdB | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (3/27); new base T (30/13); total (33/40) |
| Fisher's exact test for biased strand distribution p-value = 2.80e-07 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGAATCCTG > minE/1910087‑1910206
|
gagGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:270717/69‑1 (MQ=255)
gagGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:1803969/69‑1 (MQ=255)
gagGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:3010259/69‑1 (MQ=255)
gagGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:1178540/69‑1 (MQ=255)
gagGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:2046845/69‑1 (MQ=255)
gagGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:1237226/69‑1 (MQ=255)
gagGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:2341575/69‑1 (MQ=255)
gagGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:423446/69‑1 (MQ=255)
agGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:573321/68‑1 (MQ=255)
agGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAgg < 1:2088097/68‑1 (MQ=255)
taaaCTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCagcagc < 1:2439285/57‑1 (MQ=255)
aaaaCTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCagcagc < 1:2366447/58‑1 (MQ=255)
aaaaCTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCagcagc < 1:2451954/58‑1 (MQ=255)
aaaaCTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCagcagc < 1:3423948/58‑1 (MQ=255)
aaaaCTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCagcagc < 1:1082157/58‑1 (MQ=255)
cTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGcc < 1:567392/69‑1 (MQ=255)
cTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGcc < 1:2309184/69‑1 (MQ=255)
cTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGcc < 1:781117/69‑1 (MQ=255)
cTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGcc < 1:2453432/69‑1 (MQ=255)
cTGCGATCCTGTTGGCTCATTCCTTGTAATAGAAAACTACCCAAAACCAGCAGCATCAGGACCAGAGcc < 1:3080747/69‑1 (MQ=255)
cTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGa < 1:1895284/69‑1 (MQ=255)
cTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGa < 1:285289/69‑1 (MQ=255)
cTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGa < 1:974340/69‑1 (MQ=255)
cTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGa < 1:3323529/69‑1 (MQ=255)
aTAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTca < 1:2615731/69‑1 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCa > 1:2508616/1‑41 (MQ=38)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:3247994/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:3286130/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:513600/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:617617/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:697386/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:77756/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:819088/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:860302/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:886244/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:906125/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2834870/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2712466/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:100498/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2705720/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:982501/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2606566/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:1044570/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:1307774/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:1311690/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:1433007/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:1484398/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:1689757/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2066093/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2067123/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:213011/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2140217/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2593714/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2547373/1‑69 (MQ=255)
tAGCAAGCTACCCAAAATCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcac > 1:2306171/1‑69 (MQ=255)
tAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTca < 1:1994340/68‑1 (MQ=255)
aGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcaca > 1:2159154/1‑69 (MQ=255)
aGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcaca > 1:1180895/1‑69 (MQ=255)
aGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTcaca > 1:768120/1‑69 (MQ=255)
aaaCTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAgg < 1:2490382/68‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:2847182/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:1105111/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:1319136/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:2790496/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:1539851/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:3134533/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:1985715/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:2026489/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:355527/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:2283285/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:303310/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGGATCCTg < 1:217901/69‑1 (MQ=255)
aaaaTCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACACGTTGGATCCTg < 1:2701123/69‑1 (MQ=255)
|
GAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCATCAGGACCAGAGCCAGTGACGAAACACCCTTTTCGCGGTTCACAGGTTGAATCCTG > minE/1910087‑1910206
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
