Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,959,153 |
C→T |
57.5% |
A306A (GCG→GCA) ‡ |
fbaA ← |
fructose‑bisphosphate aldolase, class II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,959,153 | 0 | C | T | 57.5%
| 21.5
/ 60.8
| 80 | A306A (GCG→GCA) ‡ | fbaA | fructose‑bisphosphate aldolase, class II |
| Reads supporting (aligned to +/- strand): ref base C (14/20); new base T (24/22); total (38/42) |
| Fisher's exact test for biased strand distribution p-value = 3.71e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 |
TTTCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTC > minE/1959090‑1959213
|
tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:1144551/1‑68 (MQ=255)
tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:2225171/1‑68 (MQ=255)
tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:2209122/1‑68 (MQ=255)
tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:2589353/1‑68 (MQ=255)
tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:3364083/1‑68 (MQ=255)
tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:1772682/1‑68 (MQ=255)
tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:1622108/1‑68 (MQ=255)
tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACACAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:1439971/1‑68 (MQ=255)
tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGGTTTg < 1:2959711/68‑1 (MQ=255)
tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTg < 1:1816890/68‑1 (MQ=255)
tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTg < 1:2396293/68‑1 (MQ=255)
tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTg < 1:1846286/68‑1 (MQ=255)
tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTg < 1:1687158/68‑1 (MQ=255)
cTTCGCCTTTCGGGTTTCCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:276956/68‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:2374934/51‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:1169303/51‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:489486/51‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:453229/51‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:255068/51‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:780283/51‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:1898485/51‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:2967412/51‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:2957927/51‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTgt > 1:1239236/1‑54 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTgt > 1:2734838/1‑54 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:726915/68‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:380759/68‑1 (MQ=255)
cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:1626444/68‑1 (MQ=255)
tGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:210232/45‑1 (MQ=255)
gCAGGTGAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:7250/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTTTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2980771/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:973566/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:89837/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2929410/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2787165/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:999224/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:3434956/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:733216/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2529852/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:982606/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1042252/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2278199/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2233008/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1313681/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1735115/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1771902/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1818750/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1838481/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1855275/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1905179/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:200322/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2029302/1‑55 (MQ=255)
gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2261950/1‑55 (MQ=255)
cAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCAtt < 1:218172/56‑1 (MQ=255)
gATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGggtatcggtatc < 1:293455/67‑1 (MQ=255)
aGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGtt > 1:289341/1‑69 (MQ=255)
aGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGtt > 1:1259209/1‑69 (MQ=255)
aGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGtt > 1:1822309/1‑69 (MQ=255)
aGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGtt > 1:1791243/1‑69 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1230458/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2600841/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:836738/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1397035/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1526647/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1594137/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1714090/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:618753/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1717966/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1179747/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2445671/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:3422099/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2090839/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2083953/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1845109/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2584257/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:18601/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:29207/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2792009/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1893435/68‑1 (MQ=255)
cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTACCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:3290422/68‑1 (MQ=255)
|
TTTCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTC > minE/1959090‑1959213
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
