Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,967,352 |
G→A |
42.9% |
G426G (GGC→GGT) |
tktA ← |
transketolase 1, thiamin‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,967,352 | 0 | G | A | 42.9%
| 56.9
/ 107.2
| 143 | G426G (GGC→GGT) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base G (59/22); new base A (16/45); total (76/67) |
| Fisher's exact test for biased strand distribution p-value = 3.64e-08 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 143 total aligned reads displayed.
CCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGA > minE/1967288‑1967416
|
ccATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCAc < 1:1532550/69‑1 (MQ=255)
ccATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCAc < 1:1501774/69‑1 (MQ=255)
ccATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCAc < 1:225568/69‑1 (MQ=255)
aTACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCg < 1:3375249/69‑1 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1463850/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1879283/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:456071/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:481113/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2013352/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1485918/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2834233/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1232464/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:774761/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2393314/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2632517/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2714963/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1044099/1‑69 (MQ=255)
cgtacgGCGTTACGTGCGTATTCCACGAACATAAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1310410/1‑69 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:72575/1‑65 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:2809697/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:3351895/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:2391771/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:2955332/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:194863/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:3287133/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:1009842/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:549293/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:1432079/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:684896/1‑66 (MQ=255)
cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:624917/1‑66 (MQ=255)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAACCCACCGTGCa > 1:2062800/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGc > 1:2418803/1‑64 (MQ=21)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:1754035/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:825122/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:1160558/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:1382680/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:2231823/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:2142328/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3024757/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3038353/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3117644/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3199734/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:478380/1‑65 (MQ=25)
ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAggg > 1:2326529/1‑68 (MQ=25)
gCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGga < 1:2258935/68‑1 (MQ=255)
gCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGga < 1:942403/68‑1 (MQ=255)
cGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGga < 1:1012078/67‑1 (MQ=255)
cGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTa > 1:2991911/1‑68 (MQ=255)
cGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTa > 1:2942826/1‑68 (MQ=255)
cGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTa > 1:730589/1‑68 (MQ=255)
cGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTa > 1:2703656/1‑68 (MQ=255)
aTTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCaa < 1:509513/69‑1 (MQ=255)
ttCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCaa < 1:600615/68‑1 (MQ=255)
ttCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAAt > 1:770545/1‑69 (MQ=255)
ttCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAAt > 1:2183218/1‑69 (MQ=255)
aaCATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCTGGGAGATACCGTTAGCaa < 1:3328905/61‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:406813/69‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:944055/69‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:871819/69‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:617727/69‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:1671513/69‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:1889498/69‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:3280343/69‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:2941636/69‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:2998896/69‑1 (MQ=255)
gAAGGTTGAGGTATACGGCAGAAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:1166760/69‑1 (MQ=255)
ggTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAac > 1:505274/1‑69 (MQ=255)
ggTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAac > 1:2326937/1‑69 (MQ=255)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:745490/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:541657/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:582793/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:2900614/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:149563/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1363670/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1303948/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1989937/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:112568/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:829233/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:871977/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1060310/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:2346268/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1569656/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:2072864/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:3017136/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:3303047/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1768548/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:384504/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:391417/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1684888/68‑3 (MQ=21)
ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:470258/68‑3 (MQ=21)
cAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:172187/69‑1 (MQ=255)
cAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:2166205/69‑1 (MQ=255)
cAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:1967337/69‑1 (MQ=255)
aGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:268659/68‑1 (MQ=255)
ggAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:2282483/1‑69 (MQ=255)
ggAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:201847/1‑69 (MQ=255)
aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:1548391/1‑67 (MQ=255)
aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:3429054/1‑67 (MQ=255)
aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:2862896/1‑67 (MQ=255)
aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:1562746/1‑67 (MQ=255)
|
CCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGA > minE/1967288‑1967416
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
