Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,970,359 |
G→A |
51.0% |
L145L (CTG→TTG) |
speB ← |
agmatinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,970,359 | 0 | G | A | 51.0%
| ‑3.7
/ 94.5
| 102 | L145L (CTG→TTG) | speB | agmatinase |
| Reads supporting (aligned to +/- strand): ref base G (31/19); new base A (7/45); total (38/64) |
| Fisher's exact test for biased strand distribution p-value = 4.58e-07 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 |
Only 100 of 105 total aligned reads displayed.
GAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACC > minE/1970291‑1970422
|
gAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAg > 1:245730/1‑69 (MQ=255)
gAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAg > 1:124877/1‑69 (MQ=255)
aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCAt < 1:2451185/69‑1 (MQ=255)
aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCAt < 1:1142694/69‑1 (MQ=255)
aGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCAt < 1:552676/69‑1 (MQ=255)
gCCGTGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCAtttt > 1:1753014/1‑69 (MQ=255)
gCCGTGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCAtttt > 1:1112333/1‑69 (MQ=255)
gCCGTGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCAtttt > 1:817956/1‑69 (MQ=255)
gCCGTGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCAtttt > 1:2514388/1‑69 (MQ=255)
gCCGTGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCAtttt > 1:1289773/1‑69 (MQ=255)
gCCGTGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCAtttt > 1:2928915/1‑69 (MQ=255)
gCCGTGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCAtttt > 1:305247/1‑69 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:32810/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:1849621/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:236146/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:2709940/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:3153102/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:1052721/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:40637/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:7738/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:830361/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:843088/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:936784/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:941592/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:1058394/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:11975/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:1583200/69‑1 (MQ=255)
tGGTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATATTGCCg < 1:441462/69‑1 (MQ=255)
tGGGCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:2211694/69‑1 (MQ=255)
ggTCAAATTCACAACCGTTAGCGTAGGTATCGGTGTGGGCATCAAAGTGTACCAACGCCATTTTGCCg < 1:1698364/68‑1 (MQ=255)
ttCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCtt > 1:1837137/1‑69 (MQ=255)
ttCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCtt > 1:2755800/1‑69 (MQ=255)
cacaACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTc > 1:1231747/1‑68 (MQ=255)
cacaACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCg > 1:1004493/1‑69 (MQ=255)
cacaACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCg > 1:1956779/1‑69 (MQ=255)
cacaACAGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCg > 1:438920/1‑69 (MQ=255)
caACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGc < 1:2892205/68‑1 (MQ=255)
caACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGc < 1:1270855/68‑1 (MQ=255)
caACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGc < 1:1413585/68‑1 (MQ=255)
caACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGc < 1:2237393/68‑1 (MQ=255)
cGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAg < 1:884316/69‑1 (MQ=255)
ggTGTGTGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:436223/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCTCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagctgcag > 1:1519271/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:3248715/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:3387983/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:1870991/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:2878211/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:557729/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:1596995/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:85395/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:247791/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:2451133/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:1668823/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:2346351/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:2458651/1‑69 (MQ=255)
ggTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcag > 1:2425417/1‑69 (MQ=255)
gtgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAgcagcggcagcg > 1:1057252/1‑68 (MQ=255)
gtgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGt > 1:1636957/1‑69 (MQ=255)
gtgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGt > 1:343168/1‑69 (MQ=255)
gtgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGt > 1:2343811/1‑69 (MQ=255)
gtgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGt > 1:1339582/1‑69 (MQ=255)
gtgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGt > 1:2426057/1‑69 (MQ=255)
gtgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAACAGCGGAAGCGt > 1:1187205/1‑69 (MQ=255)
cGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACaa < 1:2925833/69‑1 (MQ=255)
cGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACaa < 1:3003341/69‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCTCGCAGCAGCGGCAGCGTAACAAAg < 1:2871122/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:603138/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:1842109/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:469727/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:707788/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:734482/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:798696/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:3307292/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:2272679/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:3243484/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:3166264/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:2208655/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:251282/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:1480436/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:3098964/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:2560700/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:3047176/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:2077667/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:2180333/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:2604381/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:2627269/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:1475515/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:2701839/67‑1 (MQ=255)
aaaGTGTACCAACGCCATTTTGCCGAAATGCTTCGCATGAGCCCGCAGCAGCGTCAGCGTAACAAAg < 1:3411204/67‑1 (MQ=255)
tgtACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTc < 1:584321/68‑1 (MQ=255)
gtACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac < 1:562538/69‑1 (MQ=255)
gtACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac < 1:1584356/69‑1 (MQ=255)
gtACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac < 1:1164103/69‑1 (MQ=255)
gtACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac < 1:1120726/69‑1 (MQ=255)
gtACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac < 1:1902453/69‑1 (MQ=255)
gtACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac < 1:1316948/69‑1 (MQ=255)
gtACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcac < 1:234199/69‑1 (MQ=255)
tACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcacc > 1:3121805/1‑69 (MQ=255)
tACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTcacc > 1:1584214/1‑69 (MQ=255)
aCCAACGCCATTTTGCCGAAATGCTTCGCATGAGCGCGCAGCAGCGGCAGCGTAACAAAg < 1:1923844/60‑1 (MQ=255)
|
GAACATAGTGCCGTGGTCAAATTCACAACCGTTCGCATAGGTATCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACC > minE/1970291‑1970422
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
