Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,987,066	A→G	38.0%	H219H (CAT→CAC)	ansB ←	periplasmic L‑asparaginase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,987,066	0	A	G	38.0%	35.6 / 45.3	71	H219H (CAT→CAC)	ansB	periplasmic L‑asparaginase II
Reads supporting (aligned to +/- strand): ref base A (31/13); new base G (11/16); total (42/29)
Fisher's exact test for biased strand distribution p-value = 2.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTT  >  minE/1986998‑1987134

tAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTa                                                                      >  1:1006591/1‑69 (MQ=255)

    tataAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCt                                                                  <  1:2839745/69‑1 (MQ=255)

          cAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTg                                                            >  1:1672317/1‑69 (MQ=255)

          cAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTg                                                            >  1:2488577/1‑69 (MQ=255)

          cAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTg                                                            >  1:276603/1‑69 (MQ=255)

          cAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTg                                                            >  1:1694766/1‑69 (MQ=255)

          cAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTg                                                            >  1:3325630/1‑69 (MQ=255)

          cAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTg                                                            >  1:3377579/1‑69 (MQ=255)

          cAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTg                                                            >  1:692814/1‑69 (MQ=255)

          cAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTg                                                            >  1:742070/1‑69 (MQ=255)

           aaTGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGc                                                           <  1:3052328/69‑1 (MQ=255)

              gCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCgg                                                        >  1:735802/1‑69 (MQ=255)

                      tCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCggg                                                       >  1:230536/1‑62 (MQ=255)

                      tCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCggg                                                       >  1:2660590/1‑62 (MQ=255)

                      tCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCggg                                                       >  1:1828350/1‑62 (MQ=255)

                      tCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCggg                                                       >  1:3182031/1‑62 (MQ=255)

                      tCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCgg                                                        >  1:2146014/1‑61 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:3186810/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:2585911/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:387053/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:2030374/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:2692422/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:105158/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:1255278/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:3321722/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:1769901/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTa                                            >  1:1567993/1‑68 (MQ=255)

                           aGTTCATTCAGCTTAGAGACATCGAACGGCGTGTCGCTGGTGTGCTTACGTGCC‑GGGTACGTTGGTAg                                           >  1:2073426/1‑68 (MQ=255)

                                   caGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCtt                                   >  1:2894493/1‑69 (MQ=255)

                                   caGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCtt                                   >  1:2301292/1‑69 (MQ=255)

                                   caGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCtt                                   >  1:509154/1‑69 (MQ=255)

                                              cATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGaa                        >  1:2675514/1‑69 (MQ=255)

                                              cATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGaa                        >  1:999020/1‑69 (MQ=255)

                                              cATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGaa                        >  1:2570172/1‑69 (MQ=255)

                                              cATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGaa                        >  1:926271/1‑69 (MQ=255)

                                              cATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGaa                        >  1:2172885/1‑69 (MQ=255)

                                                 cGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGt                     >  1:257021/1‑69 (MQ=255)

                                                 cGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGt                     >  1:1313076/1‑69 (MQ=255)

                                                 cGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGt                     >  1:640065/1‑69 (MQ=255)

                                                 cGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGt                     >  1:2615751/1‑69 (MQ=255)

                                                 cGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGt                     >  1:2329585/1‑69 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:1191071/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:1446185/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:150972/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:1425128/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:1685137/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:114768/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:1043050/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:2171164/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:2235658/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:2652967/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:2595921/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:208358/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:868286/69‑1 (MQ=255)

                                                 cGAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACCTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:1043819/69‑1 (MQ=255)

                                                  gAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:2637750/69‑1 (MQ=255)

                                                  gAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:1455442/69‑1 (MQ=255)

                                                  gAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:3292428/69‑1 (MQ=255)

                                                  gAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:3101124/69‑1 (MQ=255)

                                                  gAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:1296992/69‑1 (MQ=255)

                                                  gAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:2927329/69‑1 (MQ=255)

                                                  gAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:1780537/69‑1 (MQ=255)

                                                  gAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:724096/69‑1 (MQ=255)

                                                  gAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:809599/69‑1 (MQ=255)

                                                  gAACGGCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:317566/68‑1 (MQ=255)

                                                   aacggCGTGTCGCTGGTGTGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGt                     <  1:1611093/64‑1 (MQ=255)

                                                     tGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAAcc                 <  1:1877028/69‑1 (MQ=255)

                                                       gCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAAcc                 >  1:3406160/1‑67 (MQ=255)

                                                       gCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAAcc                 >  1:3210630/1‑67 (MQ=255)

                                                       gCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAAcc                 >  1:292804/1‑67 (MQ=255)

                                                           gtCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAgg           >  1:2614844/1‑69 (MQ=255)

                                                                   tATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTa                    <  1:2601029/52‑1 (MQ=255)

                                                                    aTGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGtt  >  1:1578399/1‑69 (MQ=255)

                                                                    aTGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGtt  >  1:1590864/1‑69 (MQ=255)

                                                                    aTGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGtt  <  1:2770857/69‑1 (MQ=255)

                                                                    aTGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGtt  >  1:1849926/1‑69 (MQ=255)

                                                                    aTGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGtt  >  1:2747982/1‑69 (MQ=255)

                                                                    aTGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGtt  >  1:2266090/1‑69 (MQ=255)

TAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTT  >  minE/1986998‑1987134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A