Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,987,531 |
T→C |
26.0% |
L64L (CTA→CTG) |
ansB ← |
periplasmic L‑asparaginase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,987,531 | 0 | T | C | 26.0%
| 104.5
/ 35.2
| 92 | L64L (CTA→CTG) | ansB | periplasmic L‑asparaginase II |
| Reads supporting (aligned to +/- strand): ref base T (48/20); new base C (22/2); total (70/22) |
| Fisher's exact test for biased strand distribution p-value = 5.04e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTG > minE/1987465‑1987594
|
aTTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAg > 1:2434283/1‑69 (MQ=255)
aTTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAg > 1:843496/1‑69 (MQ=255)
aTTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAg > 1:3204337/1‑69 (MQ=255)
aTTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAg > 1:1532010/1‑69 (MQ=255)
ttATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGt < 1:2665741/69‑1 (MQ=255)
ttATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGt < 1:2853530/69‑1 (MQ=255)
ttATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGt < 1:1486874/69‑1 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCtttt > 1:408731/1‑64 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGtt > 1:2620362/1‑68 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:996662/1‑69 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:2215833/1‑69 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:2139675/1‑69 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:1886598/1‑69 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:3208390/1‑69 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:1123337/1‑69 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:507910/1‑69 (MQ=255)
aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGAAATGTCTTTTAGTTg > 1:1208726/1‑69 (MQ=255)
cATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCAc < 1:3050328/69‑1 (MQ=255)
cATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCAc < 1:2453616/69‑1 (MQ=255)
ccGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGa < 1:3395006/69‑1 (MQ=255)
atTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:156288/69‑1 (MQ=255)
atTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:222840/69‑1 (MQ=255)
atTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:3049464/69‑1 (MQ=255)
atTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:3337327/69‑1 (MQ=255)
ttCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:1440053/68‑1 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCtttt < 1:244699/37‑1 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCtttt < 1:1988819/37‑1 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTa > 1:2689205/1‑38 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:2644251/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:680615/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:612432/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:2818299/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:366330/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:2872762/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:3206587/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1105818/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:2399513/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1448924/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1274082/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1872285/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1719500/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1944416/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1269966/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:234892/1‑69 (MQ=255)
cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1597039/1‑69 (MQ=255)
cACTACCAGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:3133770/1‑69 (MQ=255)
cTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:1272214/64‑1 (MQ=255)
tAACGTTCGCAATGTCTTTTAGTTGCGGCAGCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:345563/1‑69 (MQ=255)
tAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:2683782/1‑69 (MQ=255)
tAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:1740392/1‑69 (MQ=255)
tAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:545449/1‑69 (MQ=255)
tAACGTTCGCAATGTCCTTCAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCaa < 1:2067361/57‑1 (MQ=255)
tAACGTTCGCAATGTCCTTCAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCaa < 1:2628781/57‑1 (MQ=255)
aaCGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:687544/1‑68 (MQ=255)
aaCGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:2806792/1‑68 (MQ=255)
gTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTa < 1:2226694/69‑1 (MQ=255)
cGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACt > 1:38681/1‑52 (MQ=255)
cGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACt > 1:2365500/1‑52 (MQ=255)
cGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACt > 1:2276528/1‑52 (MQ=255)
cGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTa > 1:560734/1‑66 (MQ=255)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:41301/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:545743/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:709168/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:836192/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1396192/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:867009/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:3379114/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:936675/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1697325/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:3261777/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2387882/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:3204049/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2424349/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2192905/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:3202565/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2011172/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1982762/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2847171/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2936026/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:17880/1‑39 (MQ=37)
cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1827816/1‑39 (MQ=37)
cAATGACCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1509455/1‑39 (MQ=25)
gaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:132769/67‑1 (MQ=255)
aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:2702240/68‑1 (MQ=255)
aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:3424011/68‑1 (MQ=255)
aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:2231626/68‑1 (MQ=255)
aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:88299/68‑1 (MQ=255)
aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:10188/68‑1 (MQ=255)
gTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:1177671/65‑1 (MQ=255)
tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCGCTGTGTAGTTAGATTTg > 1:2566263/1‑69 (MQ=255)
tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:317675/1‑69 (MQ=255)
tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:3194385/1‑69 (MQ=255)
tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:1436163/1‑69 (MQ=255)
tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:3252358/1‑69 (MQ=255)
tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:977032/1‑69 (MQ=255)
|
ATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTG > minE/1987465‑1987594
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
