Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,993,837 |
T→C |
46.9% |
I377T (ATT→ACT) |
nupG → |
nucleoside transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,993,837 | 0 | T | C | 46.9%
| 1.6
/ 54.4
| 64 | I377T (ATT→ACT) | nupG | nucleoside transporter |
| Reads supporting (aligned to +/- strand): ref base T (9/25); new base C (15/15); total (24/40) |
| Fisher's exact test for biased strand distribution p-value = 7.15e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTT > minE/1993770‑1993900
|
ggCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCAtt < 1:2800700/69‑1 (MQ=255)
tCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTAcc < 1:2212684/68‑1 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCTAAAACGGCACTACCGACTGGCAg > 1:374088/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTAcc > 1:2666023/1‑60 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:1426522/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:2006810/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:2189478/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:1772706/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:2549695/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:2601300/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:1436357/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:2773734/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:2922787/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:1173929/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:3386596/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:687349/1‑69 (MQ=255)
aTCCTCGGCGGCATCGTGAGCGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAg > 1:964028/1‑69 (MQ=255)
tCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGa > 1:2353839/1‑69 (MQ=255)
cggcggCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCTGACCg < 1:2339676/68‑1 (MQ=255)
gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg > 1:642237/1‑54 (MQ=255)
gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg > 1:1970069/1‑54 (MQ=255)
gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg > 1:96325/1‑54 (MQ=255)
gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg > 1:2039189/1‑54 (MQ=255)
gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg > 1:1663847/1‑54 (MQ=255)
gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg > 1:3297661/1‑54 (MQ=255)
gTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAg > 1:2189329/1‑54 (MQ=255)
gAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGAtttt > 1:2051656/1‑69 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTct < 1:3008174/68‑2 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:2810172/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:117217/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:1421420/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:2843570/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:3126880/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:1001325/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:2578786/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:1762259/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:2210093/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:1849119/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:841373/68‑1 (MQ=255)
cGGTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:948939/68‑1 (MQ=255)
ggTAAAGTGGTTGAGATGTACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:1930717/67‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGATCGTATGGTTGATTTTCGCTGGTTa < 1:70642/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCTCTGGTTa < 1:1123483/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:49341/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:1943535/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:809679/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:1203594/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:2916939/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:1301331/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:2947070/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:1862185/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:1723797/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:2591891/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:1815639/68‑1 (MQ=255)
gttgttGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTa < 1:2270046/68‑1 (MQ=255)
tACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTg < 1:3114249/69‑1 (MQ=255)
tACACCCAAAACGGCACTACCGACTGGCAGACCGTATGGCTGATTTTCg < 1:3347994/49‑1 (MQ=39)
cGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGtt < 1:1114555/69‑1 (MQ=255)
cGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGtt < 1:2079528/69‑1 (MQ=255)
cGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGtt < 1:2122383/69‑1 (MQ=255)
cGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGtt < 1:2569538/69‑1 (MQ=255)
cGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGtt < 1:591047/69‑1 (MQ=255)
cGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGtt < 1:510875/69‑1 (MQ=255)
cGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGtt < 1:1145404/69‑1 (MQ=255)
cGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGtt < 1:3425593/69‑1 (MQ=255)
|
GGCTTCGGCTGTATCCTCGGCGGCATCGTGAGCGGTAAAGTTGTTGAGATGTACACCCAAAACGGCATTACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTT > minE/1993770‑1993900
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
