Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,006,379 |
G→A |
39.4% |
T182T (ACC→ACT) |
hybB ← |
predicted hydrogenase 2 cytochrome b type component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,006,379 | 0 | G | A | 39.4%
| 40.9
/ 52.2
| 81 | T182T (ACC→ACT) | hybB | predicted hydrogenase 2 cytochrome b type component |
| Reads supporting (aligned to +/- strand): ref base G (20/29); new base A (18/14); total (38/43) |
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
ATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACA > minE/2006325‑2006443
|
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAgcgc < 1:2753730/69‑1 (MQ=255)
aTGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAgcgc < 1:1707761/69‑1 (MQ=255)
gCCCGCCTAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCcat > 1:1863398/1‑69 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1045145/69‑1 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1644915/69‑1 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1616519/69‑1 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:1345899/69‑1 (MQ=255)
gaTCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATgaagaa < 1:2961699/69‑1 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGgcag > 1:1777331/1‑41 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATaaagaa > 1:2298012/1‑68 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1985450/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2952348/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2966846/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2911097/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:80504/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2208772/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:2267352/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:584893/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1360952/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1095666/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1138461/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:899179/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1441565/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1734151/1‑69 (MQ=255)
atcatcAGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATAAAGAAc > 1:1445248/1‑69 (MQ=255)
ataatcaGCGATCCCATTGAAGACTGGTGCATAGTTGGCAGCAGCGCACCAAGCGCGATGATaaagaa > 1:2473503/4‑68 (MQ=255)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2901254/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:157013/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:477388/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:287622/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2300039/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3349989/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3215600/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3192870/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:8186/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:790864/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:795274/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:2018400/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:80399/37‑1 (MQ=37)
catcaGCGATCCCATTGAAGACTGGTGCATAGTTGgc < 1:3074979/37‑1 (MQ=37)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:328097/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc < 1:402178/69‑1 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:258815/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1193713/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1226388/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1510496/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1836348/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:1918847/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:197059/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2160748/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:596133/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2323968/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:591350/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2506644/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAcc > 1:2595053/1‑69 (MQ=255)
aGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCAc > 1:3317296/1‑68 (MQ=255)
gCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATtaagaa < 1:1412031/61‑1 (MQ=255)
gAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGc > 1:656510/1‑69 (MQ=255)
gAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGc > 1:25827/1‑69 (MQ=255)
gAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGc > 1:1155155/1‑69 (MQ=255)
gAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGc > 1:3046225/1‑69 (MQ=255)
gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:3314243/67‑1 (MQ=255)
gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:3353415/67‑1 (MQ=255)
gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:301749/67‑1 (MQ=255)
gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:405202/67‑1 (MQ=255)
gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:474401/67‑1 (MQ=255)
gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:2069024/67‑1 (MQ=255)
gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:2655817/67‑1 (MQ=255)
gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:2179907/67‑1 (MQ=255)
gTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGc < 1:635449/67‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:2420345/69‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:1988584/69‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:2140330/69‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:3263811/69‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGaca < 1:563337/69‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:2255329/68‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:2806253/68‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:1857192/68‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:1314024/68‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:3196616/68‑1 (MQ=255)
gCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGac < 1:986471/68‑1 (MQ=255)
|
ATGCACCTTGTAGCCCGCCGAGATCATCAGCGACCCCATTGAAGACTGGTGCATGGTCGGCAGCAGCGCACCGAGCGCGATGATGAAGAACATCACCTTGTTTAGTCGCTGTAGCGACA > minE/2006325‑2006443
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
