Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,007,034 |
A→G |
31.5% |
I289I (ATT→ATC) ‡ |
hybA ← |
hydrogenase 2 4Fe‑4S ferredoxin‑type component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,007,034 | 0 | A | G | 31.5%
| 81.9
/ 48.1
| 95 | I289I (ATT→ATC) ‡ | hybA | hydrogenase 2 4Fe‑4S ferredoxin‑type component |
| Reads supporting (aligned to +/- strand): ref base A (44/21); new base G (19/11); total (63/32) |
| Fisher's exact test for biased strand distribution p-value = 8.16e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01 |
CGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCA > minE/2006976‑2007101
|
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTctg < 1:910981/69‑3 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTc > 1:3384911/1‑67 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTc > 1:1772431/1‑67 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:105606/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:1982066/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:3320038/69‑1 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:427512/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:1645665/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:597354/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2069923/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:155206/69‑1 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:784762/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:2174470/69‑1 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:826113/69‑1 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:865440/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:1408643/69‑1 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:3315969/69‑1 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:1348982/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:235211/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:2421767/69‑1 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2600987/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2614432/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2709212/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:2994149/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg > 1:975574/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTAAACAGGGTGTGTTGGACATGTTCgg > 1:109026/1‑69 (MQ=255)
cGGTTAAGCCCGCCAGCACAGCCAGT‑GTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCGGa > 1:1966919/1‑69 (MQ=255)
ggTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:324552/68‑1 (MQ=255)
ggTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:3042716/68‑1 (MQ=255)
ggTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGGACATGTTCgg < 1:2365528/68‑1 (MQ=255)
tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:3175634/1‑69 (MQ=255)
tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1890557/1‑69 (MQ=255)
tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1892838/1‑69 (MQ=255)
tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:889957/1‑69 (MQ=255)
tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1294219/1‑69 (MQ=255)
tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1391043/1‑69 (MQ=255)
tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:424940/1‑69 (MQ=255)
tAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:1124058/1‑69 (MQ=255)
tAAGCCCGCCAGCACAGCCAGTGGAAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAAcg > 1:2214784/1‑69 (MQ=255)
cacaGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTc > 1:404893/1‑51 (MQ=255)
cacaGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTa > 1:1856500/1‑67 (MQ=255)
cacaGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTa > 1:2180853/1‑67 (MQ=255)
aGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAg < 1:1217913/69‑1 (MQ=255)
gTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAg < 1:1953281/64‑1 (MQ=255)
gCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGtt > 1:79026/1‑69 (MQ=255)
gCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGtt > 1:955446/1‑69 (MQ=255)
gCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGtt > 1:2331771/1‑69 (MQ=255)
atGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTa > 1:1150134/1‑48 (MQ=255)
atGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGc > 1:286309/1‑69 (MQ=255)
gggTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAtttt < 1:1090773/69‑1 (MQ=255)
gggTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAtttt < 1:299472/69‑1 (MQ=255)
gggTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAtttt < 1:122617/69‑1 (MQ=255)
gggTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAtttt < 1:2609215/69‑1 (MQ=255)
tgtTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaa > 1:2420142/1‑69 (MQ=255)
tgtTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaa > 1:884816/1‑69 (MQ=255)
tgtTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaa > 1:2984889/1‑69 (MQ=255)
tgtTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaa > 1:1735054/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGt > 1:3127181/1‑43 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGt > 1:3062999/1‑43 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGt > 1:925769/1‑43 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGt > 1:302880/1‑43 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGAt > 1:2240079/1‑59 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATaaag < 1:2799241/69‑3 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:3131671/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1089899/69‑1 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:644939/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:3323726/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:2533203/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1113145/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1204832/69‑1 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1272321/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1285003/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1354870/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1419154/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1475527/69‑1 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:153832/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1540040/69‑1 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:163398/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1718824/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:1764248/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:1780045/69‑1 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:198313/69‑1 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:2202648/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:2440496/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:2484651/69‑1 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:2949304/69‑1 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCAAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:3124878/1‑69 (MQ=255)
ttGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGACCAGTTTCGGCAGGTCGAGATTTTCATAAgg < 1:640484/69‑1 (MQ=255)
ttGAATATTTTCGGAACGCGCACCAGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAgg > 1:2221693/1‑69 (MQ=255)
ttGAATATTTTCCGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGTCAGGTCGAGATTTTCATAAgg > 1:2256112/1‑69 (MQ=255)
gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGTca < 1:2053138/69‑1 (MQ=255)
gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGca < 1:399343/69‑1 (MQ=255)
gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGca < 1:1680641/69‑1 (MQ=255)
gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGca < 1:1569817/69‑1 (MQ=255)
gAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGca < 1:1128442/69‑1 (MQ=255)
|
CGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCA > minE/2006976‑2007101
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
