Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,008,482 |
C→T |
51.8% |
L180L (CTG→CTA) |
hybO ← |
hydrogenase 2, small subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,008,482 | 0 | C | T | 51.8%
| ‑2.2
/ 75.1
| 83 | L180L (CTG→CTA) | hybO | hydrogenase 2, small subunit |
| Reads supporting (aligned to +/- strand): ref base C (37/3); new base T (24/19); total (61/22) |
| Fisher's exact test for biased strand distribution p-value = 1.51e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 |
GAGGAAGTTGTGCGGGTTCGGCGGGCAGCCCGGAATATTGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACC > minE/2008425‑2008538
|
gAGGAAGTTGTGCGGGTTCGGCGGGCAGCCCGGAATATTGATAACGGTTTTGCCTGGCAGAACTTCTTg < 1:1897480/69‑1 (MQ=255)
gAGGAAGTTGTGCGGGTTCGGCGGGCAGCCCGGAATATTGATAACGGTTTTGCCTGGCAGAACTTCTTg < 1:1901309/69‑1 (MQ=255)
gAGGAAGTTGTGCGGGTTCGGCGGGCAGCCCGGAATATTGATAACGGTTTTGCCTGGCAGAACTTCTTg < 1:1370793/69‑1 (MQ=255)
aggAATATTGATATCGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2716013/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGACCTTCTTGCAGGCTGa > 1:2932339/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2265294/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:3087551/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2847357/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2843458/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:278818/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:3308924/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2779446/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2725373/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2719676/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2356452/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2553821/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:2645189/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:1835236/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:3349371/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:823171/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:770057/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:612400/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:381929/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:1637732/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:426411/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:588223/2‑47 (MQ=255)
aggAATATTGATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGa > 1:1315513/2‑47 (MQ=255)
atatTGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGc > 1:54026/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:963914/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:709885/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:478763/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:308409/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:35639/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:3243558/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:355814/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:1061380/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:129223/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:2690779/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:1950297/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:1886122/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:17092/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:1677261/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:1522513/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:2023390/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:1226696/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:1137748/1‑68 (MQ=255)
ttGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGc > 1:2534686/1‑68 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:54421/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:3209604/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:428708/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:1069579/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:905631/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:917890/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:3246664/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:1020980/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:2330669/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:2326164/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:3092540/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:2299334/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:2186187/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:2826791/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:2179964/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:29812/69‑1 (MQ=255)
gATAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:2886562/69‑1 (MQ=255)
aTAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:3092570/68‑1 (MQ=255)
aTAACGGTTTTGCCCGGTAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGc < 1:1139934/68‑1 (MQ=255)
aaCGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAac > 1:463710/1‑69 (MQ=255)
aaCGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAac > 1:998048/1‑69 (MQ=255)
aaCGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAac > 1:2031726/1‑69 (MQ=255)
aaCGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAac > 1:1415688/1‑69 (MQ=255)
aaCGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAac > 1:3101713/1‑69 (MQ=255)
aaCGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAac > 1:1726993/1‑69 (MQ=255)
aaCGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAac > 1:3105792/1‑69 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCa > 1:1714977/1‑55 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACAcc > 1:299287/1‑69 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACAcc > 1:2993621/1‑69 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACAcc > 1:2013215/1‑69 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACAcc > 1:1084463/1‑69 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACAcc > 1:1965241/1‑69 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACAcc > 1:843527/1‑69 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACAcc > 1:2775354/1‑69 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACAcc > 1:3183930/1‑69 (MQ=255)
ggTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACAcc > 1:3168596/1‑69 (MQ=255)
|
GAGGAAGTTGTGCGGGTTCGGCGGGCAGCCCGGAATATTGATAACGGTTTTGCCTGGCAGAACTTCTTGCAGGCTGACTGCGCCAGTTGGGTTAACTCCAGCTGCGGCAACACC > minE/2008425‑2008538
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
