Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,026,083 A→C 23.5% V53V (GTT→GTG)  plsC ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,026,0830AC23.5% 96.0 / 29.6 68V53V (GTT→GTG) plsC1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (22/30);  new base C (14/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.62e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGTTCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGTGGCCACATG  >  minE/2026015‑2026142
                                                                    |                                                           
ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:3171847/1‑69 (MQ=255)
ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:2473377/1‑69 (MQ=255)
ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:2290745/1‑69 (MQ=255)
ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:2492909/1‑69 (MQ=255)
ttgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:922403/1‑69 (MQ=255)
 tgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             <  1:697058/68‑1 (MQ=255)
 tgttCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             <  1:2863510/68‑1 (MQ=255)
     cTGGTGGTTAGCAATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGCAGGTTTGCGGCACTCCACtt                                                         <  1:2849073/68‑1 (MQ=255)
      tggtggTTAGCAATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGCAGGTTTGCGGCACTCCACtt                                                         <  1:1086279/67‑1 (MQ=255)
        gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCa                                                      >  1:2435182/1‑68 (MQ=255)
        gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg                                                     >  1:89299/1‑69 (MQ=255)
        gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg                                                     >  1:2263169/1‑69 (MQ=255)
        gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg                                                     >  1:258952/1‑69 (MQ=255)
        gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg                                                     >  1:2891435/1‑69 (MQ=255)
        gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg                                                     >  1:3139856/1‑69 (MQ=255)
        gtggtTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAg                                                     >  1:3216169/1‑69 (MQ=255)
                       gatagCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAgcggc                                        >  1:70398/1‑67 (MQ=255)
                       gatagCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAgcggc                                        >  1:2184120/1‑67 (MQ=255)
                       gatagCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAgcggc                                        >  1:207070/1‑67 (MQ=255)
                       gatagCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAgcggc                                        >  1:1434321/1‑67 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:420788/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:71915/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:650791/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:2403317/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:2384089/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:1400889/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:1458153/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:219496/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:2600205/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:2033353/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:3264506/1‑43 (MQ=255)
                          agCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCa                                                             >  1:1724531/1‑43 (MQ=255)
                             aTTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACg                                <  1:3248247/69‑1 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:2524675/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:2696406/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:589093/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:34105/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:3347250/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:3328055/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:1131464/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:1329084/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:1597981/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:1616941/1‑68 (MQ=255)
                                gCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGc                              >  1:2109869/1‑68 (MQ=255)
                                   gTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAATCAGCGGCGCAAGACGGCCaaa                          <  1:1610365/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGGCAAACa                        <  1:1361266/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:250744/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:992567/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:948585/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:1188259/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:1336899/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:3352703/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:3056169/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:1725450/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:211478/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:2705186/69‑1 (MQ=255)
                                     aGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:2631474/69‑1 (MQ=255)
                                      gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAAct                        <  1:9166/68‑2 (MQ=255)
                                      gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:2857404/68‑1 (MQ=255)
                                      gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:1807576/68‑1 (MQ=255)
                                      gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:2232495/68‑1 (MQ=255)
                                      gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:927853/68‑1 (MQ=255)
                                      gCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACa                        <  1:2168344/68‑1 (MQ=255)
                                        tttCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACata                      <  1:1475626/68‑1 (MQ=255)
                                        tttCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACata                      <  1:713901/68‑1 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCaaa              >  1:2529612/4‑66 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAgg            >  1:2651517/4‑68 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAgg            >  1:881776/4‑68 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAgg            >  1:1634744/4‑68 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:853215/4‑69 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:1073609/4‑69 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:2514644/4‑69 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:2507776/4‑69 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:34158/4‑69 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:1627967/4‑69 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:1675382/4‑69 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:1676702/4‑69 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTCAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:2675137/4‑69 (MQ=255)
                                                  tgcaGGTTTGCGGCACTCCACTTTAAGGCCGAAAAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           >  1:587672/4‑69 (MQ=255)
                                                  tGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           <  1:2588707/69‑1 (MQ=255)
                                                  tGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           <  1:2644831/69‑1 (MQ=255)
                                                    tAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGt           <  1:162485/67‑1 (MQ=255)
                                                            cGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGTGGCCACATg  <  1:1527494/68‑1 (MQ=255)
                                                            cGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGTGGCCACATg  <  1:1325138/68‑1 (MQ=255)
                                                            cGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGTGGCCACATg  <  1:3007201/68‑1 (MQ=255)
                                                            cGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGTGGCCACATg  <  1:2993064/68‑1 (MQ=255)
                                                            cGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGTGGCCACATg  <  1:2279743/68‑1 (MQ=255)
                                                                    |                                                           
TTGTTCTGGTGGTTAGCGATATAGATAGCATTGCCGTAGCTTTCAGCGTCTGTAGGTTTACGGCACTCAACTTTCAGGCCAAACAGCGGCGCAAGACGGCCAAACATATGCCCAAAGGTGGCCACATG  >  minE/2026015‑2026142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A