Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,036,339 |
A→G |
41.1% |
K157K (AAA→AAG) |
ygiC → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,036,339 | 0 | A | G | 41.1%
| 32.3
/ 64.8
| 90 | K157K (AAA→AAG) | ygiC | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (21/32); new base G (19/18); total (40/50) |
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCA > minE/2036279‑2036397
|
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcga > 1:3152481/1‑69 (MQ=255)
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcga > 1:676055/1‑69 (MQ=255)
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcga > 1:2017441/1‑69 (MQ=255)
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcga > 1:2063577/1‑69 (MQ=255)
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcga > 1:1720780/1‑69 (MQ=255)
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcga > 1:1565984/1‑69 (MQ=255)
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcga > 1:2320050/1‑69 (MQ=255)
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcga > 1:1229322/1‑69 (MQ=255)
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcga > 1:254998/1‑69 (MQ=255)
tCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTgatcg > 1:103215/1‑68 (MQ=255)
ttAACTCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCt > 1:2343121/1‑69 (MQ=255)
ttAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCt > 1:1367525/1‑69 (MQ=255)
ttAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCt > 1:491124/1‑69 (MQ=255)
aaCGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTc > 1:2610355/1‑69 (MQ=255)
aaCGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTc > 1:2931198/1‑69 (MQ=255)
aaCTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTg > 1:3048031/1‑69 (MQ=255)
aaCTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTg > 1:1521321/1‑69 (MQ=255)
aaCTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTg > 1:2919771/1‑69 (MQ=255)
tGCCGTAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTg < 1:2644300/69‑1 (MQ=255)
tGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTg < 1:1466657/69‑1 (MQ=255)
tGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTg < 1:150295/69‑1 (MQ=255)
tGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTg < 1:2171793/69‑1 (MQ=255)
tGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTg < 1:3048000/69‑1 (MQ=255)
tGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTg < 1:3296028/69‑1 (MQ=255)
gAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAg < 1:3198696/69‑1 (MQ=255)
aGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:2252682/69‑1 (MQ=255)
aGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:3342313/69‑1 (MQ=255)
aGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:2158342/69‑1 (MQ=255)
aGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:863594/69‑1 (MQ=255)
aGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:324277/69‑1 (MQ=255)
aGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:1863169/69‑1 (MQ=255)
aGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:1324150/69‑1 (MQ=255)
aGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:3416464/69‑1 (MQ=255)
gggCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:1960240/68‑1 (MQ=255)
gCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATg < 1:1192694/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCTAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:2498135/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATGGCTTCGTTGAGCTGCGTGAACAGTTTGGCTTcc < 1:598027/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:1186608/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:321276/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:1242769/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:125600/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:3253249/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:325340/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:2420013/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:472924/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:1529857/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:2236149/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:1182799/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:1605443/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:1166083/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:338565/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:3190337/69‑1 (MQ=255)
aCCAGTTTAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:1978607/69‑1 (MQ=255)
aCCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGt < 1:1300169/60‑1 (MQ=255)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:881993/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:336815/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:47179/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:3263440/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:3411040/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:332788/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:3372823/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:1227271/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:267378/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:2598489/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:127538/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:2430376/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:2175833/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:102259/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:2010844/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:1925768/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:1410256/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:1116988/1‑46 (MQ=39)
tAACAGTTTGCAAGAAAAGCTGATCGATCGCTTCGTTGAGCTGCGt > 1:1652926/1‑46 (MQ=39)
tAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgc > 1:3112033/1‑68 (MQ=255)
aCAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgctg < 1:561235/68‑1 (MQ=255)
cAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgctgc < 1:680807/68‑1 (MQ=255)
cAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgctgc < 1:2465681/68‑1 (MQ=255)
cAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCATCCAGTtgctgc < 1:1880922/68‑1 (MQ=255)
aGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:3347876/58‑1 (MQ=255)
cTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:1904285/55‑1 (MQ=255)
cTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:1917411/55‑1 (MQ=255)
cTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:377939/55‑1 (MQ=255)
cTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTcc < 1:503295/55‑1 (MQ=255)
cTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCAtctc < 1:614470/69‑1 (MQ=255)
cTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCAtctc < 1:100415/69‑1 (MQ=255)
cTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCAtctc < 1:1555188/69‑1 (MQ=255)
cTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCAtctc < 1:2112381/69‑1 (MQ=255)
cTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCAtctc < 1:2881489/69‑1 (MQ=255)
tGCAAGAAAAACTGATCGATCGCTTCGTTGATCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCa > 1:1410442/1‑69 (MQ=255)
tGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCa > 1:3368756/1‑69 (MQ=255)
|
TCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCA > minE/2036279‑2036397
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
