Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,053,383 |
G→A |
43.7% |
G260G (GGG→GGA) |
cca → |
fused tRNA nucleotidyl transferase and 2'3'‑cyclic phosphodiesterase/2'nucleotidase/phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,053,383 | 0 | G | A | 43.7%
| 21.6
/ 60.9
| 80 | G260G (GGG→GGA) | cca | fused tRNA nucleotidyl transferase and 2'3'‑cyclic phosphodiesterase/2'nucleotidase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (19/26); new base A (17/18); total (36/44) |
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAG > minE/2053317‑2053444
|
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:848570/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:3178288/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:3152080/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:2960182/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:2870916/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:2802085/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:2794888/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:2429855/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:2260144/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:2061596/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:1840916/69‑1 (MQ=255)
ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:1745191/69‑1 (MQ=255)
gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCt < 1:1096241/68‑1 (MQ=255)
tCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgc < 1:151279/69‑1 (MQ=255)
tGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtc < 1:1348057/68‑1 (MQ=255)
tGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtc < 1:232610/68‑1 (MQ=255)
tGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtc < 1:2287795/68‑1 (MQ=255)
tGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtc < 1:464950/68‑1 (MQ=255)
tGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtc < 1:725648/68‑1 (MQ=255)
gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAg > 1:1617521/1‑52 (MQ=255)
gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAg > 1:1296212/1‑52 (MQ=255)
gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAg > 1:3101815/1‑52 (MQ=255)
gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAg > 1:3105890/1‑52 (MQ=255)
gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAg > 1:1458505/1‑52 (MQ=255)
gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAg > 1:453486/1‑52 (MQ=255)
gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAg > 1:800622/1‑52 (MQ=255)
gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAg > 1:1884507/1‑52 (MQ=255)
tCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc > 1:778673/1‑69 (MQ=255)
tCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc > 1:1539891/1‑69 (MQ=255)
tCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc < 1:1178357/69‑1 (MQ=255)
tCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc < 1:2203032/69‑1 (MQ=255)
tCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc < 1:3178340/69‑1 (MQ=255)
tCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc < 1:3257565/69‑1 (MQ=255)
tCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc > 1:1960090/1‑69 (MQ=255)
ccGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatc < 1:1478942/68‑1 (MQ=255)
cGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatca < 1:3047859/68‑1 (MQ=255)
tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc > 1:2616315/1‑69 (MQ=255)
tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc > 1:1964394/1‑69 (MQ=255)
tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc > 1:2266793/1‑69 (MQ=255)
tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc > 1:794124/1‑69 (MQ=255)
tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc > 1:2737251/1‑69 (MQ=255)
tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc > 1:1032969/1‑69 (MQ=255)
tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc > 1:2801904/1‑69 (MQ=255)
tCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggt < 1:2050657/68‑1 (MQ=255)
ttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcg > 1:3294204/1‑69 (MQ=255)
gATCTCTGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:441068/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGtcat > 1:1349049/1‑45 (MQ=37)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:942928/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:925286/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:3056728/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:601998/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:753797/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:2436517/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:211063/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:210378/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:2247627/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:2302922/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:2325875/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:2329782/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:2440356/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:1745103/1‑67 (MQ=255)
gATCTCGGTAAAGGACTGACGCCGCCAGAAATTTGGCCACGTCATCATGGTCATGGCCCGGCGGgtg > 1:2635322/1‑67 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGTCGGGTGTGAAGTTAg < 1:811403/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:182081/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:1615137/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:1980920/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:1504074/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:180411/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:904269/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:1334773/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:702239/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:2118679/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:237321/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:1290842/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:2530560/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:2839180/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:2953406/68‑1 (MQ=255)
tAAAGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGGGTGAAGTTAg < 1:2977978/68‑1 (MQ=255)
aaaGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:2399757/67‑1 (MQ=255)
aaaGGACTGACGCCGCCAGAACTTTGGCCACGTCATCATGGTCATGGCCCGGCGGGTGTGAAGTTAg < 1:2529298/67‑1 (MQ=255)
|
GGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAG > minE/2053317‑2053444
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
