Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,086,868 |
G→A |
52.3% |
E215K (GAA→AAA) |
yqjG → |
predicted S‑transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,086,868 | 0 | G | A | 52.3%
| 0.7
/ 76.7
| 86 | E215K (GAA→AAA) | yqjG | predicted S‑transferase |
| Reads supporting (aligned to +/- strand): ref base G (25/16); new base A (36/9); total (61/25) |
| Fisher's exact test for biased strand distribution p-value = 6.11e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 |
AAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAG > minE/2086812‑2086927
|
aaGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCAc > 1:1856614/1‑69 (MQ=255)
aaGCTGGTTTTGCCA‑CAGCCAGGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACg > 1:500080/1‑69 (MQ=255)
aaGCTGGTTTTGCCA‑CAGCCAGGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACg > 1:496257/1‑69 (MQ=255)
agccagGAAGCTTACGACGAGGCTGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:297897/69‑1 (MQ=255)
agccagGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:2390070/69‑1 (MQ=255)
agccagGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:2595925/69‑1 (MQ=255)
agccagGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:2174429/69‑1 (MQ=255)
agccagGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:799574/69‑1 (MQ=255)
agccagGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:593029/69‑1 (MQ=255)
agccagGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:3388054/69‑1 (MQ=255)
agccagGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:3388683/69‑1 (MQ=255)
agccagGAAGCTTACGACGAGGCGGTGGCGAAACTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:92310/69‑1 (MQ=255)
gccagGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:2274246/68‑1 (MQ=255)
gccagGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAg < 1:872363/69‑1 (MQ=255)
aaGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAg > 1:2862762/1‑68 (MQ=255)
aaGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGc > 1:591809/1‑69 (MQ=255)
aaGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGc > 1:1092562/1‑69 (MQ=255)
aaGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGc > 1:254596/1‑69 (MQ=255)
aaGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGc > 1:876641/1‑69 (MQ=255)
aaGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGc > 1:1836495/1‑69 (MQ=255)
aaGCCTACGACGAGGCGTTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:3220395/1‑49 (MQ=37)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAAt > 1:3104729/1‑36 (MQ=37)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:3091027/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:745117/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:2869391/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:3104002/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:855023/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:2601000/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:3180964/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:3349985/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:2517461/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:496504/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:1046140/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:1667206/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:1462278/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:1788599/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:1190831/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:2226783/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGCGCGACt > 1:120396/1‑49 (MQ=38)
aaGCCTACGACGAGGCGGTGGCGAAAGTGTTTAAATCGCTGGAGCGACt > 1:1559154/1‑49 (MQ=37)
ttACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCt > 1:488466/1‑36 (MQ=255)
ttACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTa < 1:1376793/58‑1 (MQ=255)
ttACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATc > 1:2482359/1‑68 (MQ=255)
ttACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATc > 1:2263092/1‑68 (MQ=255)
ttACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATc > 1:2268366/1‑68 (MQ=255)
ttACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATc > 1:2570840/1‑68 (MQ=255)
ttACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATc > 1:358267/1‑68 (MQ=255)
ttACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATc > 1:1800903/1‑68 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGa > 1:549510/1‑39 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTa < 1:1824154/68‑1 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTa < 1:2038609/68‑1 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTa < 1:2107965/68‑1 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTAc > 1:1547892/1‑69 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTAc > 1:724597/1‑69 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTAc > 1:1551505/1‑69 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTAc > 1:1504772/1‑69 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTAc > 1:123991/1‑69 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTAc > 1:3240772/1‑69 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTAc > 1:1692510/1‑69 (MQ=255)
gacgaGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTAc > 1:1800644/1‑69 (MQ=255)
gTGGCGAAAGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGc < 1:2391327/69‑1 (MQ=255)
gTGGCGAAAGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGc < 1:1393137/69‑1 (MQ=255)
gTGGCGAAAGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGc < 1:24715/69‑1 (MQ=255)
gTGGCGAAAGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGc < 1:2239759/69‑1 (MQ=255)
gTGGCGAAAGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGc < 1:2574738/69‑1 (MQ=255)
gTGGCGAAAGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGc < 1:366354/69‑1 (MQ=255)
gTGGCGAAAGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGc < 1:639723/69‑1 (MQ=255)
gTGGCGAAAGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGc < 1:337538/69‑1 (MQ=255)
gTGGCGAAAGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGc < 1:783654/69‑1 (MQ=255)
ggCGAAAGTGTTTGAATCGCTGGCACGACTCGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCaa < 1:3276427/69‑1 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCa > 1:845534/1‑67 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:1118078/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:1010921/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:1327848/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:519403/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:366492/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:351842/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:3404085/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:3357949/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:3352757/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:1766405/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:3072137/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:180907/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:2595199/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:2588373/1‑68 (MQ=255)
aaGTGTTTAAATCGCTGGCGCGACTGGAACAGATTTTAGGTCAGCACCGTTACCTGACCGGCAACCAg > 1:2541444/1‑68 (MQ=255)
|
AAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGAGGCGGTGGCGAAAGTGTTTGAATCGCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAG > minE/2086812‑2086927
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
