Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,125,208 |
G→A |
61.9% |
A52A (GCG→GCA) |
yhbQ → |
predicted endonuclease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,125,208 | 0 | G | A | 61.9%
| 37.1
/ 49.1
| 76 | A52A (GCG→GCA) | yhbQ | predicted endonuclease |
| Reads supporting (aligned to +/- strand): ref base G (8/21); new base A (17/30); total (25/51) |
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01 |
GCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCG > minE/2125142‑2125251
|
gcaCCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGcc < 1:2476492/69‑1 (MQ=255)
gcaCCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGcc < 1:2194983/69‑1 (MQ=255)
gcaCCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGcc < 1:2824390/69‑1 (MQ=255)
gcaCCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGcc < 1:1644014/69‑1 (MQ=255)
caCCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGcc < 1:14030/68‑1 (MQ=255)
gCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCg < 1:2827989/69‑1 (MQ=255)
gCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCg < 1:1500355/69‑1 (MQ=255)
gCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCg < 1:3291631/69‑1 (MQ=255)
ggCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGcc < 1:2518876/59‑1 (MQ=255)
cAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCg < 1:3299810/69‑1 (MQ=255)
cAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCg < 1:1015388/69‑1 (MQ=255)
cAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCg < 1:2595519/69‑1 (MQ=255)
cAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCg < 1:2192851/69‑1 (MQ=255)
cAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCg < 1:2866096/69‑1 (MQ=255)
cAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCg < 1:2074028/69‑1 (MQ=255)
cAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCg < 1:996641/69‑1 (MQ=255)
cAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCg < 1:1335378/69‑1 (MQ=255)
cAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCg < 1:140205/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCa > 1:8778/1‑46 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:581764/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:975997/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:905326/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:2557751/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:2579985/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:2776617/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:739373/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:716493/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:383327/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:289391/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:2968209/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:3071744/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:3103140/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:422974/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:408656/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:2517033/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:336920/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:3385887/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:3411335/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:2410044/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:1136730/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:123437/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:1284761/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:1467095/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:1539620/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:1539906/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:1569341/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:1652695/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:1846510/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:2026424/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:2083461/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:2171894/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:2510464/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:2219726/69‑1 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:2134749/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:2158392/1‑69 (MQ=255)
aGCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGt > 1:2534507/1‑68 (MQ=255)
aGCACTGCGAGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt > 1:1459933/1‑69 (MQ=255)
gCACTGCGCGGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:79587/68‑1 (MQ=255)
cgGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGTTACGGGca < 1:3348938/67‑2 (MQ=255)
cgGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGTTACGGGca < 1:789619/67‑2 (MQ=255)
cgGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGTTACGGGca < 1:2091692/67‑2 (MQ=255)
cgGGAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGAGCGTTCGCTGGCGTTACGGGca < 1:1719608/67‑2 (MQ=255)
gggAAGGGAGAACTAACGCTGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGTTACGGGca < 1:3159536/66‑2 (MQ=255)
gggAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGaa < 1:3310126/69‑1 (MQ=255)
gggAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGaa < 1:2670418/69‑1 (MQ=255)
ggAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGaa < 1:428741/68‑1 (MQ=255)
gAAGGGAGAACTAACGCAGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:2147224/57‑1 (MQ=255)
gagaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATcgc > 1:796888/1‑68 (MQ=255)
gagaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATcgc > 1:1712180/1‑68 (MQ=255)
agaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATcgcg > 1:2874244/1‑68 (MQ=255)
agaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATcgcg > 1:1411817/1‑68 (MQ=255)
agaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATcgcg > 1:3399992/1‑68 (MQ=255)
agaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATcgcg > 1:232490/1‑68 (MQ=255)
agaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATcgcg > 1:1177361/1‑68 (MQ=255)
agaACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATcgcg > 1:2119987/1‑68 (MQ=255)
tGGCGTTTTCCGCACCAGTGGGCGATCGTTCGCTGGCGtt < 1:228266/40‑1 (MQ=38)
cgcgCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGaa < 1:216125/40‑1 (MQ=255)
|
GCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCG > minE/2125142‑2125251
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
