Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,243,253 |
G→T |
20.4% |
V115V (GTC→GTA) |
nfi ← |
endonuclease V |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,243,253 | 0 | G | T | 20.4%
| 184.2
/ 31.5
| 93 | V115V (GTC→GTA) | nfi | endonuclease V |
| Reads supporting (aligned to +/- strand): ref base G (59/15); new base T (12/7); total (71/22) |
| Fisher's exact test for biased strand distribution p-value = 1.41e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 |
Only 100 of 106 total aligned reads displayed.
AGAGCCGTTTTTTCGCCACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAGCA > minE/2243192‑2243323
|
agagCCGTTTTTTCGCCACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGTCTGGCGACGCCaa < 1:2285851/69‑1 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:1517756/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:2183922/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:2180075/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:2368963/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:2504255/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:1807434/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:3163085/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:1329473/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:720447/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:378381/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:684733/1‑69 (MQ=255)
ttttCGCTACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAg > 1:39435/1‑69 (MQ=255)
ttttCGCCACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAg > 1:2849295/1‑69 (MQ=255)
ccaATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCgaga > 1:2823557/1‑67 (MQ=255)
ccaATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGAt > 1:90508/1‑68 (MQ=255)
ccaATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGAt > 1:2207627/1‑68 (MQ=255)
ccaATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGAt > 1:1980720/1‑68 (MQ=255)
ccaATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGAt > 1:594958/1‑68 (MQ=255)
ccaATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGAt > 1:537408/1‑68 (MQ=255)
caATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATcc < 1:1324130/69‑1 (MQ=255)
caATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATcc < 1:927123/69‑1 (MQ=255)
caATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATcc < 1:239255/69‑1 (MQ=255)
caATGGGCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATcc < 1:921436/69‑1 (MQ=255)
ggTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAg < 1:1742736/53‑1 (MQ=255)
ggTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAg < 1:11153/53‑1 (MQ=255)
gTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATg > 1:1380516/1‑68 (MQ=255)
gTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATg > 1:3166337/1‑68 (MQ=255)
cGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGAcc < 1:1696251/69‑1 (MQ=255)
cGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGAcc < 1:2971893/69‑1 (MQ=255)
cGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGAcc < 1:2042841/69‑1 (MQ=255)
ggCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGAcc < 1:1592650/68‑1 (MQ=255)
ggCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCa > 1:2786239/1‑69 (MQ=255)
ggCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCa > 1:2476185/1‑69 (MQ=255)
ggCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCa > 1:2401627/1‑69 (MQ=255)
ggCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCa > 1:770578/1‑69 (MQ=255)
ggCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCa > 1:1929743/1‑69 (MQ=255)
ggCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCa > 1:1918851/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGAc > 1:2487373/1‑68 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:1078969/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:139136/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:1308496/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:3288353/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:1125487/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:505167/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:580586/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:582878/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa < 1:3118133/69‑1 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:612978/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:663617/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:666399/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:715394/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:95569/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:1003195/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:2358211/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:2362780/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:827334/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:2745890/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:2735728/1‑69 (MQ=255)
tCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACa > 1:1851237/1‑69 (MQ=255)
caccaATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACaaa > 1:1048473/1‑69 (MQ=255)
aTAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTa < 1:3361888/69‑1 (MQ=255)
aTAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTa < 1:3244259/69‑1 (MQ=255)
aTAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTa < 1:2022591/69‑1 (MQ=255)
aTAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTa < 1:645879/69‑1 (MQ=255)
tAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTa < 1:812948/68‑1 (MQ=255)
tAAGCCAAAATGGCTGGCTACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTa < 1:719046/68‑1 (MQ=255)
aaGCCAAAATGGCTGGCGACTCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTaaa > 1:3380161/1‑69 (MQ=255)
aaGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGAt > 1:2749753/1‑44 (MQ=255)
aaGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTaaa > 1:3426960/1‑69 (MQ=255)
aaGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTaaa > 1:1785852/1‑69 (MQ=255)
aaGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTaaa > 1:2868714/1‑69 (MQ=255)
aGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGa > 1:2513095/1‑57 (MQ=255)
aGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAAt > 1:3355141/1‑69 (MQ=255)
aGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAAt > 1:2268799/1‑69 (MQ=255)
aGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAAt > 1:875840/1‑69 (MQ=255)
aGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAAt > 1:3060549/1‑69 (MQ=255)
aGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAAt > 1:450475/1‑69 (MQ=255)
aGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAAt > 1:408900/1‑69 (MQ=255)
aGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACAATAAAt > 1:1370937/1‑69 (MQ=255)
gctggcGACGCCAAGACGGCGAGGATGCGAGATCCCa > 1:236864/1‑37 (MQ=255)
gctggcGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCtttt > 1:1101245/1‑68 (MQ=255)
gctggcGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCtttt > 1:1432618/1‑68 (MQ=255)
gctggcGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCtttt > 1:1565444/1‑68 (MQ=255)
gctggcGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCtttt > 1:1569654/1‑68 (MQ=255)
gctggcGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCttt > 1:1909862/1‑67 (MQ=255)
gctggcGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCttt > 1:1272752/1‑67 (MQ=255)
tggcGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGc < 1:385079/68‑1 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAGTCCGGCTTTTGCGACAg > 1:3369212/1‑69 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg > 1:2553227/1‑69 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg > 1:834751/1‑69 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg > 1:3038649/1‑69 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg < 1:2993493/69‑1 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg > 1:2652114/1‑69 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg < 1:2253008/69‑1 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg > 1:1875052/1‑69 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg < 1:1377269/69‑1 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg > 1:1315605/1‑69 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg > 1:1284661/1‑69 (MQ=255)
gACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAg < 1:1018133/69‑1 (MQ=255)
|
AGAGCCGTTTTTTCGCCACGCCAATGGTCGGCACATCCACCAATAAGCCAAAATGGCTGGCGACGCCAAGACGGCGAGGATGCGAGATCCCATGACCATCGACAAACACTAAATCCGGCTTTTGCGACAGCA > minE/2243192‑2243323
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
