Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,269,123 |
A→G |
24.7% |
P61P (CCT→CCC) |
birA ← |
bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase and DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,269,123 | 0 | A | G | 24.7%
| 131.2
/ 39.4
| 109 | P61P (CCT→CCC) | birA | bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase and DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding |
| Reads supporting (aligned to +/- strand): ref base A (44/38); new base G (21/6); total (65/44) |
| Fisher's exact test for biased strand distribution p-value = 4.07e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 109 total aligned reads displayed.
ACACTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGAA > minE/2269064‑2269187
|
acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:106467/69‑1 (MQ=255)
acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:3097860/69‑1 (MQ=255)
acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:764333/69‑1 (MQ=255)
acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:50261/69‑1 (MQ=255)
acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:3200387/69‑1 (MQ=255)
acacTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:1266194/69‑1 (MQ=255)
cTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTAt > 1:207912/1‑69 (MQ=255)
cTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTAt > 1:1911981/1‑69 (MQ=255)
cTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTAt > 1:740311/1‑69 (MQ=255)
cTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTAt > 1:3245272/1‑69 (MQ=255)
aCCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATcc > 1:549349/1‑69 (MQ=255)
aCCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATcc > 1:1281618/1‑69 (MQ=255)
aCCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATcc > 1:2616405/1‑69 (MQ=255)
aCCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATcc > 1:2880623/1‑69 (MQ=255)
ccAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTg < 1:1321519/56‑1 (MQ=255)
cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:1223618/69‑1 (MQ=255)
cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:533760/69‑1 (MQ=255)
cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:1171572/69‑1 (MQ=255)
cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:2129430/69‑1 (MQ=255)
cAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCgg < 1:2076700/69‑1 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTa > 1:1463163/1‑54 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:428389/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1845793/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:2517044/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:2420829/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:528529/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:2656005/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:299926/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:2959785/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1036552/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1739309/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1178682/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1597465/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:736102/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTaa > 1:1452602/1‑67 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:807507/1‑66 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:2996610/1‑66 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:846025/1‑66 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:3072797/1‑66 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:1204265/1‑66 (MQ=255)
aaTATCTTTTCGGCATTAAGTAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTa > 1:2010183/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGTCTGTATCCTTTACCCGGAACGGTAAAGa > 1:1156613/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTTTATCCTTTACCAGGAAc > 1:845339/1‑58 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:3385530/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:1738614/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:1126352/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:304790/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:3171324/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:120788/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:1803552/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:2300763/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:711975/1‑66 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACa > 1:499037/1‑68 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACa > 1:2760100/1‑68 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACa > 1:3352118/1‑68 (MQ=255)
tCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACa > 1:295026/1‑68 (MQ=255)
cTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:3210307/1‑69 (MQ=255)
cTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:188621/1‑69 (MQ=255)
tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:416781/64‑1 (MQ=255)
tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:3259458/64‑1 (MQ=255)
tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:668437/64‑1 (MQ=255)
tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:2642416/64‑1 (MQ=255)
tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:1536519/64‑1 (MQ=255)
tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:2641626/64‑1 (MQ=255)
tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa < 1:1356086/64‑1 (MQ=255)
tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc < 1:1911044/68‑1 (MQ=255)
tGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCa > 1:2929981/1‑69 (MQ=255)
tAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGa > 1:2008346/1‑60 (MQ=255)
tAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGc > 1:2064127/1‑69 (MQ=255)
gCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGcc < 1:1402068/68‑1 (MQ=255)
gCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGcc < 1:3328072/68‑1 (MQ=255)
gCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGcc < 1:1971956/68‑1 (MQ=255)
gCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATACTTTACCCGGAACGGTAAAGACATCAACGcc < 1:3203999/68‑1 (MQ=255)
ttAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTa > 1:2733955/1‑51 (MQ=255)
ttAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCa > 1:1029377/1‑68 (MQ=255)
aaGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTc > 1:1202521/1‑69 (MQ=255)
aaGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTc > 1:1504353/1‑69 (MQ=255)
aaGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTc > 1:1727879/1‑69 (MQ=255)
aaGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTc > 1:2290585/1‑69 (MQ=255)
tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:897581/68‑1 (MQ=255)
tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:2955934/68‑1 (MQ=255)
tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:905108/68‑1 (MQ=255)
tAACTGGATTGGCTCGGGCAAGCTGTATCCTTTACCCGGAACAGTAAATACATCAACGCCCCAGTCAc < 1:2702973/68‑1 (MQ=255)
aaCTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGc < 1:1361341/69‑1 (MQ=255)
ggATAGGCTCAGGCAGGCTTTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:2620037/69‑1 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACAt > 1:1318542/1‑48 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:464187/1‑49 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:3043532/1‑49 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:2153685/1‑49 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:2566918/1‑49 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATc > 1:889826/1‑49 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:3013579/69‑1 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:2727903/69‑1 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:1363424/69‑1 (MQ=255)
ggATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:2024964/69‑1 (MQ=255)
gATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAgtg < 1:899145/68‑1 (MQ=255)
cTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGaa < 1:2669189/68‑1 (MQ=255)
cTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGaa < 1:1125677/68‑1 (MQ=255)
cTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGaa < 1:3252024/68‑1 (MQ=255)
cTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGaa < 1:3400513/68‑1 (MQ=255)
|
ACACTACCGCCATCCAGCTGACCCAATATCTGTTTAGCATTAAGTAACTGGATAGGCTCAGGCAGGCTGTATCCTTTACCCGGAACGGTAAAGACATCAACGCCCCAGTCACGCAGTGTCTGAA > minE/2269064‑2269187
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
