Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,269,255 |
A→G |
21.7% |
G17G (GGT→GGC) |
birA ← |
bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase and DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,269,255 | 0 | A | G | 21.7%
| 104.7
/ 21.7
| 78 | G17G (GGT→GGC) | birA | bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase and DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding |
| Reads supporting (aligned to +/- strand): ref base A (32/29); new base G (5/12); total (37/41) |
| Fisher's exact test for biased strand distribution p-value = 1.08e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
TGTTTATTAATAGCCGCCCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAC > minE/2269190‑2269319
|
tgtTTATTAATAGCCGCCCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCg > 1:826258/1‑69 (MQ=255)
gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt > 1:2343390/1‑63 (MQ=255)
gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt > 1:2060018/1‑63 (MQ=255)
gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt > 1:413533/1‑63 (MQ=255)
gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt > 1:721461/1‑63 (MQ=255)
gccgccCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTCCGCt > 1:2962367/1‑63 (MQ=255)
cTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt < 1:2185855/54‑1 (MQ=255)
cTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt < 1:1084405/54‑1 (MQ=255)
cTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt < 1:251263/54‑1 (MQ=255)
cTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCt < 1:200498/54‑1 (MQ=255)
tCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAg < 1:653288/69‑1 (MQ=255)
cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:952544/1‑69 (MQ=255)
cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:2772770/1‑69 (MQ=255)
cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:1777662/1‑69 (MQ=255)
cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:728670/1‑69 (MQ=255)
cccAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGt > 1:884603/1‑69 (MQ=255)
aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:42059/68‑1 (MQ=255)
aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:2287948/68‑1 (MQ=255)
aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:2818487/68‑1 (MQ=255)
aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:2050086/68‑1 (MQ=255)
aGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:3392865/68‑1 (MQ=255)
tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:759568/1‑51 (MQ=255)
tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:1409837/1‑51 (MQ=255)
tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:2879322/1‑51 (MQ=255)
tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:846617/1‑51 (MQ=255)
tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:184015/1‑51 (MQ=255)
tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:1575645/1‑51 (MQ=255)
tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:892279/1‑51 (MQ=255)
tCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGcaatc > 1:167284/1‑51 (MQ=255)
aCCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTgg < 1:1087752/60‑1 (MQ=255)
cGCCAGAGTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCAt < 1:327714/67‑1 (MQ=255)
cGCCAGAGTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCAt < 1:968333/67‑1 (MQ=255)
cGCCAGAGTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCAt < 1:2271826/67‑1 (MQ=255)
gagTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTg < 1:1416126/69‑1 (MQ=255)
gagTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTg < 1:3281668/69‑1 (MQ=255)
gagTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTg < 1:3212610/69‑1 (MQ=255)
gagTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTg < 1:2587799/69‑1 (MQ=255)
gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:1016201/69‑1 (MQ=255)
gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:896209/69‑1 (MQ=255)
gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:500443/69‑1 (MQ=255)
gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:3083581/69‑1 (MQ=255)
gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2734258/69‑1 (MQ=255)
gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2577900/69‑1 (MQ=255)
gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2327272/69‑1 (MQ=255)
gTGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2331205/69‑1 (MQ=255)
gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtg > 1:3031970/1‑68 (MQ=255)
gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:2788469/1‑69 (MQ=255)
gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc < 1:2019777/69‑1 (MQ=255)
gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:421142/1‑69 (MQ=255)
gTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:189070/1‑69 (MQ=255)
tGAAATTCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATACTTCATGAAATTGtc < 1:2097950/68‑1 (MQ=255)
tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:257727/1‑68 (MQ=255)
tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:2280857/1‑68 (MQ=255)
tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:1070823/1‑68 (MQ=255)
tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:1239524/1‑68 (MQ=255)
tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:2133218/1‑68 (MQ=255)
tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:2860592/1‑68 (MQ=255)
tGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGtc > 1:1986830/1‑68 (MQ=255)
aaaTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTa > 1:1029879/1‑49 (MQ=255)
aaaTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTa > 1:796557/1‑49 (MQ=255)
aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:168927/69‑1 (MQ=255)
aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:1104584/69‑1 (MQ=255)
aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:1152416/69‑1 (MQ=255)
aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:3112750/69‑1 (MQ=255)
aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:1691266/69‑1 (MQ=255)
aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:2206439/69‑1 (MQ=255)
aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:3108909/69‑1 (MQ=255)
aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:191345/69‑1 (MQ=255)
aTTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:2163263/69‑1 (MQ=255)
ttCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACg > 1:2302347/1‑40 (MQ=37)
ttCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACg > 1:777402/1‑40 (MQ=37)
ttCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGaagga > 1:2295394/1‑57 (MQ=255)
ttCGCCATTCGCTAACAGGGCGATCAATTTCAGTGGCACGGTGTTATCCTTCATGaagga > 1:3045782/1‑57 (MQ=255)
ttCGCCATTCGCTAACAGCGCGATCAATTTCAGAGGCACGGTGTTATCCt > 1:1611131/1‑50 (MQ=25)
ttCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:164792/68‑1 (MQ=255)
ttCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:464960/68‑1 (MQ=255)
ttCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:1773706/68‑1 (MQ=255)
ttCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAc < 1:234338/68‑1 (MQ=255)
|
TGTTTATTAATAGCCGCCCGGCTCATTCCCAGCGTTTCACCCAACTGCTCGCCAGAGTGAAATTCACCGTTCGCTAACAGGGCAATCAATTTCAGTGGCACGGTGTTATCCTTCATGAAATTGTCTCCAC > minE/2269190‑2269319
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
