Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,286,015 |
A→G |
37.3% |
G119G (GGT→GGC) |
argB ← |
acetylglutamate kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,286,015 | 0 | A | G | 37.3%
| 31.8
/ 40.4
| 67 | G119G (GGT→GGC) | argB | acetylglutamate kinase |
| Reads supporting (aligned to +/- strand): ref base A (35/7); new base G (16/9); total (51/16) |
| Fisher's exact test for biased strand distribution p-value = 8.47e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-02 |
TAACCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTACGGCCGC > minE/2285953‑2286083
|
tAACCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAc > 1:81346/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAct > 1:3424396/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAct > 1:1812376/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAct > 1:1778227/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAct > 1:3012168/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAct > 1:3029710/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAct > 1:3030863/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAct > 1:208930/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAct > 1:914015/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAct > 1:2148426/1‑69 (MQ=255)
aaCCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAAc > 1:858965/1‑68 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGcc > 1:2048249/1‑52 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCGTAACTCTTCATCAAGCt > 1:1180616/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:2221883/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:1688193/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:1006029/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:199293/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:26663/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:2702554/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:2941330/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:3020437/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:3181632/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:1464525/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:1358649/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:828445/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:1265692/1‑69 (MQ=255)
gCAAGCTGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCt > 1:998146/1‑69 (MQ=255)
gATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGa > 1:2564280/1‑40 (MQ=255)
gATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACttt > 1:1031148/1‑69 (MQ=255)
cTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCt > 1:3236377/1‑69 (MQ=255)
tAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGt < 1:276358/69‑1 (MQ=255)
tAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGt < 1:1921760/69‑1 (MQ=255)
tAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGt < 1:2128673/69‑1 (MQ=255)
tAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGt < 1:3073874/69‑1 (MQ=255)
tAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGt < 1:258831/69‑1 (MQ=255)
tAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGt < 1:2359161/69‑1 (MQ=255)
tAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGt < 1:3299974/69‑1 (MQ=255)
tAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGt < 1:3427310/69‑1 (MQ=255)
tAGGCGAACCTGGCTGCGCCAGTCCAACATGGCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGt < 1:977938/69‑1 (MQ=255)
ggCTGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCa > 1:2644769/1‑66 (MQ=255)
tGCGCCAGTCCGACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:2002034/1‑69 (MQ=255)
tGCGCCAGTCCAACATGACCTAACTCTTCATCAAg > 1:3240709/1‑35 (MQ=255)
tGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:325292/1‑69 (MQ=255)
tGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:3214156/1‑69 (MQ=255)
tGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:1380621/1‑69 (MQ=255)
tGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:3029363/1‑69 (MQ=255)
tGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:2721081/1‑69 (MQ=255)
tGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:2352517/1‑69 (MQ=255)
tGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:2359912/1‑69 (MQ=255)
tGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgag > 1:1518907/1‑68 (MQ=255)
gcgcCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:2755562/1‑68 (MQ=255)
gcgcCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:2238962/1‑68 (MQ=255)
gcgcCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:996200/1‑68 (MQ=255)
gcgcCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:1449809/1‑68 (MQ=255)
gcgcCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:2021770/1‑68 (MQ=255)
gcgcCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCgaga > 1:1932007/1‑68 (MQ=255)
cAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGaaacaa < 1:2903308/69‑1 (MQ=255)
cAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGaaacaa < 1:1293814/69‑1 (MQ=255)
cAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGaaacaa < 1:2230392/69‑1 (MQ=255)
cAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGaaacaa < 1:3405321/69‑1 (MQ=255)
aGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGaaacaa < 1:3115844/68‑1 (MQ=255)
aGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGaaacaa < 1:1716968/68‑1 (MQ=255)
aGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGaaacaa < 1:1112500/68‑1 (MQ=255)
ccAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCt > 1:1950088/1‑69 (MQ=255)
ccAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCt > 1:1697217/1‑69 (MQ=255)
cAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTa > 1:2291570/1‑69 (MQ=255)
cAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTa > 1:1106002/1‑69 (MQ=255)
cAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTa > 1:1592048/1‑69 (MQ=255)
aCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTACGGCCg > 1:2937438/1‑68 (MQ=255)
aCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTACGGCCGc > 1:902173/1‑69 (MQ=255)
aCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTACGGCCGc > 1:3414992/1‑69 (MQ=255)
aCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTACGGCCGc > 1:1511887/1‑69 (MQ=255)
aCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTACGGCCGc > 1:2724680/1‑69 (MQ=255)
aCCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTACGGCCGc > 1:2116909/1‑69 (MQ=255)
|
TAACCGTTCTCCAGCAAGGAGTTGATAAGCTTAGGCGAACCTGGCTGCGCCAGTCCAACATGACCTAACTCTTCATCAAGCTGGGTCACTTTGACGCTGTCGCCGTCACCGAGAAACAAACCTACGGCCGC > minE/2285953‑2286083
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
