Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,287,932 |
A→G |
65.8% |
K131K (AAA→AAG) |
argE → |
acetylornithine deacetylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,287,932 | 0 | A | G | 65.8%
| 50.3
/ 34.8
| 67 | K131K (AAA→AAG) | argE | acetylornithine deacetylase |
| Reads supporting (aligned to +/- strand): ref base A (9/14); new base G (21/23); total (30/37) |
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 |
AAAGGCTTTTTTGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAGTATGGCCGGAGCGCGT > minE/2287879‑2287995
|
aaaGGCTTTTTTGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAACTGAAAAAACCGctc > 1:1408889/1‑69 (MQ=255)
aaaGGCTTTTTTGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAACTGAAAAAACCGctc > 1:1643826/1‑69 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:2346188/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:2213243/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:2218334/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:3115964/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:2259370/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:3039867/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:229759/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:2332857/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:3146000/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:2397944/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:3032133/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:77610/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:2941056/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:262957/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:692629/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:329679/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:3301215/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:877265/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:3369070/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:897058/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:148317/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:484096/48‑1 (MQ=255)
ttttGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCt < 1:1203872/48‑1 (MQ=255)
tATCCTTGATGCGCTACGCGATGTTGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:359128/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:785130/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:3295825/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:643799/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:3401593/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:2975038/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:691444/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:610336/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:2071676/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:112134/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:130532/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:1007343/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:1534972/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:1621588/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:1919651/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:865099/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:2219164/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:2285237/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:2501863/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:2561551/1‑64 (MQ=255)
tATCCTTGATGCGCTACGCGATGTCGACGTCACGAAGCTTGCAAAACCGCTCTACATTCTGGCg > 1:2690324/1‑64 (MQ=255)
tGATGCGCTACGCGATGTCGACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCtgatg < 1:257457/68‑1 (MQ=255)
tGATGCGCTACGCGATGTCGACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCtgatg < 1:539621/68‑1 (MQ=255)
gcgcTACGCGATGTCGACTTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATgaaga < 1:686318/68‑1 (MQ=255)
gcgcTACGCGATGTCGACGTCACGAAACTGAAAAAACCGCTTTACATTCTGGCGACTGCTGATgaaga < 1:697570/68‑1 (MQ=255)
gcgcTACGCGATGTCGACGTCACGAAACTGAAAAAACCGCTGTACATTCTGGCGACTGCTGATgaaga < 1:1737918/68‑1 (MQ=255)
gcgcTACGCGATGTCGACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATgaaga < 1:662729/68‑1 (MQ=255)
gcgcTACGCGATGTCGACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATgaaga < 1:185267/68‑1 (MQ=255)
gcgcTACGCGATGTCGACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATgaaga < 1:473352/68‑1 (MQ=255)
aCGCGATGTCGACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAg < 1:975876/69‑1 (MQ=255)
aCGCGATGTCGACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAg < 1:3216340/69‑1 (MQ=255)
gACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAGTATGGCCGGa > 1:1339470/1‑69 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAg < 1:3128376/53‑1 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAg < 1:2838520/53‑1 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAg < 1:2182228/53‑1 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAg < 1:1728843/53‑1 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAGTATGGCCGGAGCGCGt > 1:337984/1‑69 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAGTATGGCCGGAGCGCGt > 1:2785235/1‑69 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAGTATGGCCGGAGCGCGt > 1:2765703/1‑69 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAGTATGGCCGGAGCGCGt > 1:2518734/1‑69 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAGTATGGCCGGAGCGCGt > 1:2514639/1‑69 (MQ=255)
aCGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAGTATGGCCGGAGCGCGt > 1:1762134/1‑69 (MQ=255)
|
AAAGGCTTTTTTGCGTTTATCCTTGATGCGCTACGCGATGTCGACGTCACGAAACTGAAAAAACCGCTCTACATTCTGGCGACTGCTGATGAAGAAACCAGTATGGCCGGAGCGCGT > minE/2287879‑2287995
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
