Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,317,231 |
T→C |
45.5% |
T302T (ACT→ACC) |
glpX → |
fructose 1,6‑bisphosphatase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,317,231 | 0 | T | C | 45.5%
| 11.3
/ 53.9
| 66 | T302T (ACT→ACC) | glpX | fructose 1,6‑bisphosphatase II |
| Reads supporting (aligned to +/- strand): ref base T (23/13); new base C (20/10); total (43/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACT > minE/2317163‑2317292
|
ttctCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACt > 1:3117930/1‑69 (MQ=255)
aCCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACg > 1:2896095/1‑69 (MQ=255)
ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGctgctg > 1:1128635/1‑68 (MQ=255)
ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:1939617/1‑69 (MQ=255)
ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:3162650/1‑69 (MQ=255)
ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:1643029/1‑69 (MQ=255)
ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:1668988/1‑69 (MQ=255)
ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:3057648/1‑69 (MQ=255)
ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:1891104/1‑69 (MQ=255)
ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:687283/1‑69 (MQ=255)
ttACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGa > 1:2392654/1‑69 (MQ=255)
aCCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATc > 1:1069149/1‑69 (MQ=255)
ctgctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCa > 1:1448809/1‑69 (MQ=255)
ggAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCa < 1:307830/64‑1 (MQ=255)
ggAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCa < 1:1973879/64‑1 (MQ=255)
cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc > 1:1129371/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:2971658/1‑69 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:1567090/1‑69 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:207581/1‑69 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAACCGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:2750290/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1860501/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:2320237/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:749649/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1994037/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1124907/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1965462/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:588358/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:2640343/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1791515/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1783734/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1752373/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:477565/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:3308910/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1574794/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1588099/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1652510/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcg > 1:585951/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcg > 1:1268304/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcg > 1:1076364/1‑68 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:865069/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1019888/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:2988054/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:564207/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:311636/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1048649/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:1117177/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:1260210/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:172526/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1421051/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:2287803/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1601868/1‑60 (MQ=255)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:3425534/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1163130/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1353095/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:3196160/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1602253/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:170617/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1750497/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1812437/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:3005781/36‑1 (MQ=16)
atCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:681831/35‑1 (MQ=16)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt > 1:1565903/1‑69 (MQ=255)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt < 1:1910828/69‑1 (MQ=255)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt < 1:863605/69‑1 (MQ=255)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt < 1:1950333/69‑1 (MQ=255)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCAACATTCGCCGCATTCAGTCCATCCACt < 1:447517/69‑1 (MQ=255)
aCTACCGAAACGCTGCTGATCCGCGGCTAGTCACGCACCATTCGCCGCa < 1:342587/49‑1 (MQ=255)
|
TTCTCTGCCACCGGTATTACCAAAGGCGATCTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACT > minE/2317163‑2317292
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
