Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,317,255 |
C→T |
30.2% |
G310G (GGC→GGT) |
glpX → |
fructose 1,6‑bisphosphatase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,317,255 | 0 | C | T | 30.2%
| 101.1
/ 45.4
| 99 | G310G (GGC→GGT) | glpX | fructose 1,6‑bisphosphatase II |
| Reads supporting (aligned to +/- strand): ref base C (21/48); new base T (20/10); total (41/58) |
| Fisher's exact test for biased strand distribution p-value = 1.61e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 |
CTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGAAATGCA > minE/2317193‑2317320
|
ctgctgGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCa > 1:1448809/1‑69 (MQ=255)
ggAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCa < 1:307830/64‑1 (MQ=255)
ggAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCa < 1:1973879/64‑1 (MQ=255)
cATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTc > 1:1129371/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:2971658/1‑69 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:1567090/1‑69 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTcgc > 1:207581/1‑69 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAACCGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:2750290/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1994037/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:2640343/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:2320237/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1791515/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1783734/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1574794/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1588099/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1965462/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1860501/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1752373/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:3308910/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:477565/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1652510/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:1124907/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:588358/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcgt > 1:749649/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcg > 1:1268304/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcg > 1:585951/1‑68 (MQ=255)
aTTAGCCGCAAAGGCAATATCGCCACCACCGAAACGCTGCTGATCCGTGGTAAATCACGCACCATTcg > 1:1076364/1‑68 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:172526/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1019888/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:2287803/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:2988054/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:311636/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:564207/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:865069/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1048649/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1421051/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:1117177/60‑1 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa > 1:1601868/1‑60 (MQ=255)
gCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCa < 1:1260210/60‑1 (MQ=255)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:3005781/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1163130/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1353095/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1812437/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1602253/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:1750497/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:3196160/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:3425534/36‑1 (MQ=16)
tatCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:170617/36‑1 (MQ=16)
atCGCCACCACCGAAACGCTGCTGATCCGTGGTaa < 1:681831/35‑1 (MQ=16)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt < 1:1910828/69‑1 (MQ=255)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt < 1:1950333/69‑1 (MQ=255)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt > 1:1565903/1‑69 (MQ=255)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACt < 1:863605/69‑1 (MQ=255)
tCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCAACATTCGCCGCATTCAGTCCATCCACt < 1:447517/69‑1 (MQ=255)
aCTACCGAAACGCTGCTGATCCGCGGCTAGTCACGCACCATTCGCCGCa < 1:342587/49‑1 (MQ=255)
ccGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGAt > 1:2176719/1‑68 (MQ=255)
ccGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGAt > 1:2957247/1‑68 (MQ=255)
ccGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGAt > 1:2952580/1‑68 (MQ=255)
ccGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGAt > 1:2923748/1‑68 (MQ=255)
ccGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGAt > 1:2892146/1‑68 (MQ=255)
ccGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGAt > 1:1007323/1‑68 (MQ=255)
ccGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGAt > 1:2065016/1‑68 (MQ=255)
ccGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGAt > 1:752014/1‑68 (MQ=255)
ccGAAACGCTGCTGATCCGCGGCAAGTCACGCACAATTCGCCGCATTCAGTCCATCCACTATCTGGAt > 1:1638469/1‑68 (MQ=255)
aaaCGCTTCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCa < 1:1601962/69‑1 (MQ=255)
aaaCGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGTATCGCa < 1:942529/69‑1 (MQ=255)
aaaCGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCa < 1:2358800/69‑1 (MQ=255)
aaaCGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCa < 1:1435157/69‑1 (MQ=255)
aaaCGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCa < 1:507691/69‑1 (MQ=255)
aaaCGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCa < 1:1962213/69‑1 (MQ=255)
aCGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCaaa < 1:1730340/69‑1 (MQ=255)
aCGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCaaa < 1:2278950/69‑1 (MQ=255)
cGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATGCACTATCTGGATCGCAAAg < 1:1620895/69‑1 (MQ=255)
cGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAg < 1:1410722/69‑1 (MQ=255)
cGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAg < 1:1908475/69‑1 (MQ=255)
cGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAg < 1:3014586/69‑1 (MQ=255)
cGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAg < 1:2489958/69‑1 (MQ=255)
cGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAg < 1:3193355/69‑1 (MQ=255)
cGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAg < 1:1749960/69‑1 (MQ=255)
cGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAg < 1:1251579/69‑1 (MQ=255)
cGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAg < 1:239519/69‑1 (MQ=255)
gctgctGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGa < 1:1137704/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTGTCTGGATCGCAAAGACCCGGa < 1:1696424/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:3139534/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:295347/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:513801/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:2849356/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:1135395/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:1418420/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:2429019/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:1489270/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:753131/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:2057186/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:1998092/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:1493258/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:962679/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:985116/69‑1 (MQ=255)
gATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAATCCATCCACTATCTGGATCGCAAAGACCCGGa < 1:1482225/69‑1 (MQ=255)
cgGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGAAATGCa < 1:1386800/69‑1 (MQ=255)
|
CTGCTGGAAGGCATTAGCCGCAAAGGCAATATCGCGACTACCGAAACGCTGCTGATCCGCGGCAAGTCACGCACCATTCGCCGCATTCAGTCCATCCACTATCTGGATCGCAAAGACCCGGAAATGCA > minE/2317193‑2317320
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
