Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,322,184 |
C→T |
69.6% |
K296K (AAG→AAA) |
sbp ← |
sulfate transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,322,184 | 0 | C | T | 69.6%
| 95.0
/ 51.1
| 105 | K296K (AAG→AAA) | sbp | sulfate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/8); new base T (49/24); total (73/32) |
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 106 total aligned reads displayed.
AAATGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTCGCGCGGACGGTAGTAGTTTTTCGCGGC > minE/2322122‑2322252
|
aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:247521/69‑1 (MQ=255)
aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:2816767/69‑1 (MQ=255)
aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:3041245/69‑1 (MQ=255)
aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:3017099/69‑1 (MQ=255)
aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:2711791/69‑1 (MQ=255)
aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:2516847/69‑1 (MQ=255)
gcgcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCg < 1:1480694/68‑1 (MQ=255)
gcgcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCg < 1:1895884/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:225763/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1593826/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2836061/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1682939/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2890327/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:522456/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:3057187/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2080950/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2118741/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2621403/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1075234/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2603125/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:3396806/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:6434/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1233818/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1238086/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:702572/68‑1 (MQ=255)
gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:859473/68‑1 (MQ=255)
tCGTCCAGCCGCCGAACTCTTCATCAATCGTGAATAACTTCCATTTTGGAAACGCATTTTCGTa < 1:1360283/64‑1 (MQ=255)
gTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1948383/62‑1 (MQ=255)
gccgAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1653276/54‑1 (MQ=255)
gAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACttt > 1:3167351/1‑55 (MQ=255)
gAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGc > 1:3258926/1‑69 (MQ=255)
gAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGc > 1:2286166/1‑69 (MQ=255)
gAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGc > 1:2144853/1‑69 (MQ=255)
ctctTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1871632/48‑1 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:338918/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:337227/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:1917545/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:2045432/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:3288167/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:850133/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:725616/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:15297/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:574825/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:212994/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:198193/1‑69 (MQ=255)
ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:504266/1‑69 (MQ=255)
catcatcaATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:1196247/3‑69 (MQ=255)
ttCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAg > 1:1636093/1‑62 (MQ=255)
ttCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgcg > 1:2856026/1‑69 (MQ=255)
ttCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgcg > 1:1110561/1‑69 (MQ=255)
tGGTGACTAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:6493/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTc > 1:2912966/1‑44 (MQ=38)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTAcc > 1:2315518/1‑50 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTc > 1:1240655/1‑52 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:3185564/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1000259/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2706807/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:284567/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2632079/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:3265779/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2588860/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2565440/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2538715/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2482081/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:384198/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2379513/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:39395/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2613746/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:441630/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1806611/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1818960/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1953841/1‑69 (MQ=255)
tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1835777/1‑69 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTcg > 1:2326908/1‑55 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1009678/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:480168/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:469930/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:447888/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:412118/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2765866/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1041631/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1075314/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1143162/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1249120/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1410032/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1511712/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1573805/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1856245/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1877970/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2158429/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2188080/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2226852/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2299495/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2341870/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2446387/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2536144/1‑63 (MQ=255)
gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2571446/1‑63 (MQ=255)
tAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTCGCGCGGACGGTAGTAGtt > 1:3339930/1‑69 (MQ=255)
aCTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTCGCGCGGACGGTAGTAGtttt > 1:1576974/1‑69 (MQ=255)
aCTTCAGCTTTGGAAACGCATTTACGTACTTTTTCGCCACCTCAGCGTcgcgcc > 1:875228/1‑53 (MQ=255)
|
AAATGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTCGCGCGGACGGTAGTAGTTTTTCGCGGC > minE/2322122‑2322252
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
