Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,334,282 |
G→A |
28.1% |
K577K (AAG→AAA) |
fdoG → |
formate dehydrogenase‑O, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,334,282 | 0 | G | A | 28.1%
| 151.8
/ 55.8
| 131 | K577K (AAG→AAA) | fdoG | formate dehydrogenase‑O, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (38/56); new base A (26/11); total (64/67) |
| Fisher's exact test for biased strand distribution p-value = 3.30e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 132 total aligned reads displayed.
AGGGCTTTAACCCTGTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTG > minE/2334215‑2334348
|
aGGGCTTTAACCCTGTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGt > 1:2366620/1‑69 (MQ=255)
aGGGCTTTAACCCTGTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGt > 1:2065589/1‑69 (MQ=255)
aGGGCTTTAACCCTGTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGt > 1:2418010/1‑69 (MQ=255)
aGGGCTTTAACCCTGTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGt > 1:1910522/1‑69 (MQ=255)
ggCTTTAACCCTGTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTc < 1:2438209/69‑1 (MQ=255)
ggCTTTAACCCTGTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTc < 1:1403861/69‑1 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:2788809/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:214170/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:2203081/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:241970/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:1923357/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:3102112/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:3178869/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:938734/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:816154/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:447790/1‑69 (MQ=255)
ttGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTc < 1:1510461/56‑1 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:1553607/1‑68 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:1673502/1‑68 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:908160/1‑68 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:2572183/1‑68 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:1123520/1‑68 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:2791965/1‑69 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:2779816/1‑69 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:2501252/1‑69 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:138028/1‑69 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:1225997/1‑69 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa > 1:3331391/1‑69 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3152646/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2181087/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:239232/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2980693/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2887270/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:43196/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3370006/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2421091/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2599246/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:94509/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3274371/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:14130/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:1105178/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:118051/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:1282944/69‑1 (MQ=255)
cGAACAAAAACAAAGTGATCGGTTGTCTGGCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3069008/67‑1 (MQ=255)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTAt > 1:2320308/1‑52 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:456776/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1680172/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2856490/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2691943/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2689155/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2958475/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1343472/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1316340/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:3394846/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:477556/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:549253/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:622798/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2104780/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:775449/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2276925/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2124770/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1063014/1‑53 (MQ=25)
acaaaaacaaaGTGATAGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1022315/1‑53 (MQ=25)
caaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactga < 1:700780/69‑1 (MQ=255)
caaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactga < 1:2116289/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:908086/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2071884/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2061423/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1241137/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:3272354/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1766353/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1471083/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1694688/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2677807/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1353794/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2922932/69‑1 (MQ=255)
aaaaacaaaGTGATCGGATGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:3217355/69‑1 (MQ=255)
aaaaacaaaGCGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1284047/69‑1 (MQ=255)
aaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAc < 1:1235507/69‑1 (MQ=255)
aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCa > 1:1191924/1‑46 (MQ=255)
aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAcc > 1:1230281/1‑69 (MQ=255)
aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAcc > 1:2304058/1‑69 (MQ=255)
aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAc < 1:1810709/68‑1 (MQ=255)
aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAc > 1:921091/1‑68 (MQ=255)
aaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3412973/56‑1 (MQ=255)
tGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACt > 1:843076/1‑69 (MQ=255)
tGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACt > 1:1731415/1‑69 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:900187/69‑1 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:213446/69‑1 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:482673/69‑1 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:1800910/69‑1 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGAACCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:828525/69‑1 (MQ=255)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTTGCAGAACCACGGt < 1:2003894/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2373874/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2217535/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:1272905/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2751002/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2152581/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2755713/66‑1 (MQ=18)
gTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTg < 1:330967/67‑1 (MQ=255)
|
AGGGCTTTAACCCTGTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTG > minE/2334215‑2334348
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
