Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,334,294 |
C→T |
30.5% |
T581T (ACC→ACT) |
fdoG → |
formate dehydrogenase‑O, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,334,294 | 0 | C | T | 30.5%
| 124.0
/ 56.9
| 121 | T581T (ACC→ACT) | fdoG | formate dehydrogenase‑O, large subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/50); new base T (26/11); total (60/61) |
| Fisher's exact test for biased strand distribution p-value = 3.09e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 |
Only 100 of 121 total aligned reads displayed.
GTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTGAACGA > minE/2334229‑2334353
|
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:938734/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:214170/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:2203081/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:1923357/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:2788809/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:1314239/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:3102112/1‑69 (MQ=255)
gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:447790/1‑69 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:2572183/1‑68 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:1673502/1‑68 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:1123520/1‑68 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:1553607/1‑68 (MQ=255)
gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:135632/1‑68 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:1225997/1‑69 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:2779816/1‑69 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:2501252/1‑69 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:2791965/1‑69 (MQ=255)
tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:138028/1‑69 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2421091/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:94509/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2411085/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:239232/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2181087/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3274371/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2599246/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:43196/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2867517/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3370006/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2887270/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2970074/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3107038/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3152646/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3233747/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:14130/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa > 1:2041725/1‑69 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:1282944/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:1105178/69‑1 (MQ=255)
cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:1227893/69‑1 (MQ=255)
cGAACAAAAACAAAGTGATCGGTTGTCTGGCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3069008/67‑1 (MQ=255)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTAt > 1:2320308/1‑52 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1135172/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2736589/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2781325/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1343472/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2958475/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:3342141/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:3394846/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2104780/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:456776/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:465582/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:477556/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:549253/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:622798/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:775449/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1063014/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2689155/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2691943/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2124770/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2276925/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1680172/1‑53 (MQ=25)
acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1727682/1‑53 (MQ=25)
acaaaaacaaaGTGATAGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1022315/1‑53 (MQ=25)
caaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactga < 1:700780/69‑1 (MQ=255)
caaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactga < 1:2116289/69‑1 (MQ=255)
aaaaacaaaGTGTTCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2449073/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:3272354/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1471083/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2061423/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2677807/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1353794/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:908086/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:91015/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1766353/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2071884/69‑1 (MQ=255)
aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2922932/69‑1 (MQ=255)
aaaaacaaaGTGATCGGATGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:3217355/69‑1 (MQ=255)
aaaaacaaaGCGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1284047/69‑1 (MQ=255)
aaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAc < 1:1235507/69‑1 (MQ=255)
aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCa > 1:1191924/1‑46 (MQ=255)
aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAcc > 1:1230281/1‑69 (MQ=255)
aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAc < 1:1810709/68‑1 (MQ=255)
aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAc > 1:921091/1‑68 (MQ=255)
aaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3412973/56‑1 (MQ=255)
tGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACt > 1:843076/1‑69 (MQ=255)
tGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACt > 1:1731415/1‑69 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:2355332/69‑1 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:900187/69‑1 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:213446/69‑1 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:1800910/69‑1 (MQ=255)
tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGAACCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:828525/69‑1 (MQ=255)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTTGCAGAACCACGGt < 1:2003894/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2217535/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2373874/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:847326/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:1258429/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:1272905/66‑1 (MQ=18)
agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2751002/66‑1 (MQ=18)
gTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTg < 1:330967/67‑1 (MQ=255)
ccTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTGAACGa > 1:681722/1‑69 (MQ=255)
ccTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTGAACGa > 1:3042427/1‑69 (MQ=255)
|
GTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTGAACGA > minE/2334229‑2334353
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
