Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,388,901 |
G→A |
34.9% |
L181L (CTC→CTT) |
pldB ← |
lysophospholipase L(2) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,388,901 | 0 | G | A | 34.9%
| 88.0
/ 66.8
| 117 | L181L (CTC→CTT) | pldB | lysophospholipase L(2) |
| Reads supporting (aligned to +/- strand): ref base G (26/50); new base A (33/8); total (59/58) |
| Fisher's exact test for biased strand distribution p-value = 2.06e-06 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 |
Only 100 of 120 total aligned reads displayed.
CGCGCCAGCGCCCGGTGCCTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAGCGCAAT > minE/2388837‑2388969
|
gcCCGGTGCCTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTCCCGTg < 1:3153257/69‑1 (MQ=255)
gTGCCTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATa < 1:950979/69‑1 (MQ=255)
gTGCCTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATa < 1:923888/69‑1 (MQ=255)
gTGCCTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATa < 1:2386073/69‑1 (MQ=255)
gTGCCTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATa < 1:1998623/69‑1 (MQ=255)
gTGCCTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATa < 1:1785217/69‑1 (MQ=255)
gTGCCTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATa < 1:356765/69‑1 (MQ=255)
ccTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAAt < 1:2066324/69‑1 (MQ=255)
atcgCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGAc < 1:2343399/66‑1 (MQ=255)
atcgCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGAc < 1:2523937/66‑1 (MQ=255)
atcgCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGAc < 1:1605799/66‑1 (MQ=255)
atcgCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGAc < 1:1574049/66‑1 (MQ=255)
atcgCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGAc < 1:1543456/66‑1 (MQ=255)
atcgCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGAc < 1:872299/66‑1 (MQ=255)
atcgCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGAc < 1:1318744/66‑1 (MQ=255)
tAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCa > 1:2950841/1‑67 (MQ=255)
tAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCAt > 1:740487/1‑68 (MQ=255)
tAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCAt > 1:2805838/1‑68 (MQ=255)
gCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACg > 1:2833810/1‑69 (MQ=255)
gCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACg > 1:1261013/1‑69 (MQ=255)
aCGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATc > 1:1356118/1‑67 (MQ=255)
aCGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCAc > 1:2985304/1‑69 (MQ=255)
aCGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCAc > 1:2302876/1‑69 (MQ=255)
aCGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCAc > 1:2714622/1‑69 (MQ=255)
aCGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCAc > 1:2848349/1‑69 (MQ=255)
aCGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCAc > 1:3366097/1‑69 (MQ=255)
aCGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCAc > 1:611406/1‑69 (MQ=255)
aCGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGACATAAATGACGGCATACGAATAAc > 1:2717597/1‑69 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAAt > 1:3227908/1‑46 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATg > 1:2773519/1‑47 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:687560/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:990772/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:3436420/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:2488900/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:856470/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:3336825/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:465051/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:3032605/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:561641/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:3253924/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:423222/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:3122756/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:3015647/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1701464/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1423945/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1377941/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1840834/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1352996/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1816051/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1763143/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1710655/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:2122756/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1646669/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:2211294/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:1666636/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATcc > 1:2293462/1‑68 (MQ=255)
aCGTGGATGCGCTTCGGACAAGTTAAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACAATc > 1:3253965/1‑67 (MQ=255)
aCGTCGATGCGCTTCGGCCCAGTTAAGGATCTGCCGTGCCATAAAt > 1:963505/1‑46 (MQ=255)
tGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATa > 1:1174714/1‑52 (MQ=255)
tGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCaaa > 1:2988434/1‑69 (MQ=255)
tGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCaaa > 1:1319249/1‑69 (MQ=255)
tGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCaaa > 1:1285452/1‑69 (MQ=255)
tGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCaaa < 1:1274247/69‑1 (MQ=255)
tGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCaaa < 1:1066052/69‑1 (MQ=255)
tGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCaaa > 1:2385664/1‑69 (MQ=255)
tGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCaa > 1:500135/1‑68 (MQ=255)
tGGATGCACTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCaaa > 1:1296819/1‑69 (MQ=255)
cgcTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTgg > 1:2303249/1‑69 (MQ=255)
gCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGt < 1:759720/68‑1 (MQ=255)
gCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGt < 1:1984336/68‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1877154/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1036174/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:952352/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:2518972/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1105659/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1107894/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1111887/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:833639/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:2673139/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:690736/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1210254/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:2166448/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:2790661/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:3129494/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:442005/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:435753/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1847144/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:4224/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:368075/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:2840499/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:3380194/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1380669/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:2886874/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1816685/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:30662/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:3067440/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:3181373/69‑1 (MQ=255)
gTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAgcgc < 1:1699246/69‑1 (MQ=255)
gAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAGCGCaa > 1:286637/1‑68 (MQ=255)
gAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAGCGCAAt > 1:248618/1‑69 (MQ=255)
|
CGCGCCAGCGCCCGGTGCCTATTGCATAGCCATCACGGAAACGTGGATGCGCTTCGGCCCAGTTGAGGATCTGCCGTGCCATAAATGACGGCATACGAATCACGATCCCAAACATTGGCGCAGTTAGCGCAAT > minE/2388837‑2388969
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
