Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,399,911 |
G→A |
55.1% |
T240T (ACC→ACT) |
uvrD ← |
DNA‑dependent ATPase I and helicase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,399,911 | 0 | G | A | 55.1%
| 5.6
/ 38.0
| 49 | T240T (ACC→ACT) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base G (11/11); new base A (18/9); total (29/20) |
| Fisher's exact test for biased strand distribution p-value = 2.60e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
TTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAG > minE/2399843‑2399979
|
ttCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCg > 1:1019677/1‑69 (MQ=255)
ttCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCg > 1:2831923/1‑69 (MQ=255)
gcgcCATCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2225/1‑57 (MQ=37)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAgtat > 1:3131803/1‑54 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAgtat > 1:9093/1‑54 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:352519/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2558791/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:246978/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2413018/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:3360753/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:213105/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:3136046/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1795127/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2850536/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:3004999/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1520159/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1397561/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:137875/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1312417/1‑57 (MQ=38)
gcgcCAGCCGTAGATTGACTGGTCGTCATAGCCAACGATCATCACTTTGCCAGTATc > 1:2603160/1‑57 (MQ=255)
ccGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATc < 1:2337523/69‑1 (MQ=255)
gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTcgcc > 1:1862475/1‑51 (MQ=255)
gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCa > 1:2613707/1‑69 (MQ=255)
gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCa > 1:3225176/1‑69 (MQ=255)
ttGACTGGTCGTCATCGGCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2000434/68‑1 (MQ=255)
ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2989401/68‑1 (MQ=255)
ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:944340/68‑1 (MQ=255)
ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:410985/68‑1 (MQ=255)
ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:521089/68‑1 (MQ=255)
ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:92951/68‑1 (MQ=255)
ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2985619/68‑1 (MQ=255)
ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2785107/68‑1 (MQ=255)
ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2690590/68‑1 (MQ=255)
ttGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCAcgcg < 1:1019526/68‑1 (MQ=255)
tGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCAcgcg < 1:613475/67‑1 (MQ=255)
tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTTTCCTgg < 1:434955/68‑1 (MQ=255)
tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:3187805/68‑1 (MQ=255)
tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:2312378/68‑1 (MQ=255)
tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:1986383/68‑1 (MQ=255)
tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:76924/68‑1 (MQ=255)
tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:1623109/68‑1 (MQ=255)
tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:1585506/68‑1 (MQ=255)
tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTCTTGGTATCCTgg < 1:3304181/68‑1 (MQ=255)
catcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGaa > 1:1106116/1‑69 (MQ=255)
catcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGaa > 1:1199722/1‑69 (MQ=255)
aCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCg > 1:407780/1‑69 (MQ=255)
ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:86416/1‑69 (MQ=255)
ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:882514/1‑69 (MQ=255)
ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:1317840/1‑69 (MQ=255)
ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:94018/1‑69 (MQ=255)
ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:2947996/1‑69 (MQ=255)
ggTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAg < 1:3009008/69‑1 (MQ=255)
|
TTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAG > minE/2399843‑2399979
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
