Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,411,465 |
C→T |
25.9% |
S245S (AGC→AGT) |
hemY → |
predicted protoheme IX synthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,411,465 | 0 | C | T | 25.9%
| 156.0
/ 47.5
| 128 | S245S (AGC→AGT) | hemY | predicted protoheme IX synthesis protein |
| Reads supporting (aligned to +/- strand): ref base C (49/45); new base T (20/13); total (70/58) |
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01 |
Only 100 of 128 total aligned reads displayed.
CTGGAACAACAGGCATGGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGTG > minE/2411409‑2411531
|
cTGGAACAACAGGCATGGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGt < 1:2108678/69‑1 (MQ=255)
cATTGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGaaaa < 1:366624/69‑1 (MQ=255)
cATGGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGaaaa < 1:2784192/69‑1 (MQ=255)
cATGGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGaaaa < 1:2823960/69‑1 (MQ=255)
cATGGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGaaaa < 1:2777185/69‑1 (MQ=255)
cATGGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGaaaa < 1:3173085/69‑1 (MQ=255)
cATGGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGaaaa < 1:113314/69‑1 (MQ=255)
cATGGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGaaaa < 1:1879876/69‑1 (MQ=255)
cATGGATTGCCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGaaaa < 1:1777360/69‑1 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGa > 1:2929137/1‑41 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:2681819/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:2693148/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:1728841/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:2729228/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:2733275/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:2511336/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:2297374/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:2068896/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:2925855/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:728277/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:899361/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:98576/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCaa > 1:990673/1‑69 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCa > 1:2932164/1‑68 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCa > 1:683269/1‑68 (MQ=255)
aTTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGAGaa > 1:55033/1‑42 (MQ=255)
ttGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCaaa < 1:787078/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:3376984/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:678038/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:268615/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:2140783/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:997223/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:1053123/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:1282067/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:1283686/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:190249/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:1748947/69‑1 (MQ=255)
tGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGTGAAGGTTTGCGTAACTGGTGGAAAAACCAAAg < 1:1795538/69‑1 (MQ=255)
ggATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAAc > 1:2008241/1‑69 (MQ=255)
ggATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAAc > 1:1629711/1‑69 (MQ=255)
gATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTTCGTAACTGGTGGAAAAACC‑AAGCCGGAAAAcgc < 1:1259908/69‑1 (MQ=255)
tCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTTGTGGAAAAACCAAAGCCGGAAAAcgc > 1:3426587/1‑68 (MQ=255)
tCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAAcgc > 1:2992883/1‑68 (MQ=255)
tCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAAcgc > 1:1006389/1‑68 (MQ=255)
tCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAAcgc > 1:1358541/1‑68 (MQ=255)
tCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAAcgc > 1:3242146/1‑68 (MQ=255)
tCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAAcgc > 1:3052726/1‑68 (MQ=255)
tCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAAcgc < 1:1577247/68‑1 (MQ=255)
tCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAAcgc > 1:2089806/1‑68 (MQ=255)
cgcgTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGtcatc < 1:2175564/69‑1 (MQ=255)
cgcgTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGtcatc < 1:3356868/69‑1 (MQ=255)
cgcgTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGtcatc > 1:2018083/1‑69 (MQ=255)
cgcgTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGtcatc < 1:1437322/69‑1 (MQ=255)
ccGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAg > 1:3307426/1‑69 (MQ=255)
gATAACGGTAGCGAATGTTTGCGTAACTGGTGGAAAAACCaaa < 1:3409652/43‑1 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:433591/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:2184925/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:1367936/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:3150558/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:3130802/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:666058/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:3085538/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:693218/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:2981919/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:2915217/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:1499863/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:1831645/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:876911/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:1891713/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:1431235/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:2450002/1‑67 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTg < 1:1014518/69‑1 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTg < 1:2874620/69‑1 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTg < 1:303168/69‑1 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTg < 1:196789/69‑1 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTg < 1:223121/69‑1 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTg < 1:2704880/69‑1 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTg < 1:3311901/69‑1 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTg < 1:3319661/69‑1 (MQ=255)
aaCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAAACAAAGCCGGAAAACGCGTCATCAGGTAGCGt > 1:2055257/1‑67 (MQ=255)
aCGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTg < 1:853884/68‑1 (MQ=255)
ggTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCa < 1:1031164/68‑1 (MQ=255)
ggTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCa < 1:2651319/68‑1 (MQ=255)
ggTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCa < 1:769316/68‑1 (MQ=255)
ggTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCa < 1:1220412/68‑1 (MQ=255)
ggTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCa < 1:1574881/68‑1 (MQ=255)
ggTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCa < 1:2257573/68‑1 (MQ=255)
ggTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCa < 1:2568727/68‑1 (MQ=255)
aGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGt > 1:1925303/1‑68 (MQ=255)
aGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGt > 1:3120733/1‑68 (MQ=255)
aGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGt > 1:2242513/1‑68 (MQ=255)
aGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGt > 1:1241044/1‑68 (MQ=255)
aGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGt > 1:3026851/1‑68 (MQ=255)
aGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGt > 1:1479141/1‑68 (MQ=255)
aGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGt > 1:2863584/1‑68 (MQ=255)
aGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGt > 1:1543833/1‑68 (MQ=255)
aGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGt > 1:950315/1‑68 (MQ=255)
gCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGTg > 1:1080798/1‑68 (MQ=255)
gCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGTg > 1:2314429/1‑68 (MQ=255)
gCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGTg > 1:205093/1‑68 (MQ=255)
|
CTGGAACAACAGGCATGGATTGGCCTGATGGATCAGGCGCGTGCCGATAACGGTAGCGAAGGTTTGCGTAACTGGTGGAAAAACCAAAGCCGGAAAACGCGTCATCAGGTAGCGTTGCAGGTG > minE/2411409‑2411531
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
