Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,425,207 C→T 39.3% P295P (CCG→CCA)  rffG ← dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,425,2070CT39.3% 67.2 / 86.4 127P295P (CCG→CCA) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (60/17);  new base T (35/15);  total (95/32)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

CTGCGGCAGCCAGCCAAGTTCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGTTCGGAGCCAG  >  minE/2425141‑2425275
                                                                  |                                                                    
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGt                                                  >  1:2568075/1‑68 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGt                                                  >  1:2149608/1‑68 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:963546/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:2723860/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:2698946/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:2742933/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:2548801/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:2998530/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:301394/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:2109846/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:2106400/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:3191956/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:3238583/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:3259737/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:3371803/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:754019/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:1153642/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:474757/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:1202312/1‑69 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTg                                                 <  1:2729314/69‑1 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTg                                                 <  1:2278307/69‑1 (MQ=255)
                   tCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTg                                                 <  1:3102146/69‑1 (MQ=255)
                   tCACGGG‑AATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg                                                 >  1:225904/1‑68 (MQ=255)
                          cAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGt                                          >  1:1116580/1‑69 (MQ=255)
                          cAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGt                                          >  1:2904257/1‑69 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAgg                                                   >  1:1870463/1‑59 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  >  1:121729/1‑60 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  >  1:2771915/1‑60 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  >  1:2345012/1‑60 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  <  1:605739/60‑1 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  >  1:1660492/1‑60 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  >  1:3136484/1‑60 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  >  1:3312132/1‑60 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  <  1:588489/60‑1 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  <  1:1360250/60‑1 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  <  1:1884875/60‑1 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  <  1:3283327/60‑1 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  >  1:1701144/1‑60 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCAACGGACGGTCAGCGACAAAGGt                                                  >  1:1484186/1‑60 (MQ=255)
                           aaTTTTCGAAGCATCAATGGCATAACGAAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  >  1:1042511/1‑60 (MQ=255)
                           aaTTTTCGAAGCATCAATGCCATAACGCAGATCATGCCCCGGACGGTCAGCGACa                                                       >  1:1213317/1‑55 (MQ=255)
                           aaTTTTCCAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt                                                  >  1:3379313/1‑60 (MQ=255)
                                   aaGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAg                                  <  1:1833509/68‑1 (MQ=255)
                                   aaGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAg                                  <  1:3042428/68‑1 (MQ=255)
                                   aaGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAg                                  <  1:2100792/68‑1 (MQ=255)
                                   aaGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAg                                  <  1:2096901/68‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:2980844/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:3092678/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:3325217/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:761733/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:2733188/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:964384/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:2620529/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:169296/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:1850690/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:2322592/69‑1 (MQ=255)
                                        tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:1057565/69‑1 (MQ=255)
                                         cAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:579176/68‑1 (MQ=255)
                                          aaTGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc                            <  1:2880467/67‑1 (MQ=255)
                                          aaTGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCAc                          >  1:855390/1‑69 (MQ=255)
                                          aaTGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCAc                          >  1:386862/1‑69 (MQ=255)
                                          aaTGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCAc                          >  1:1118714/1‑69 (MQ=255)
                                            tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc                        >  1:3325856/1‑69 (MQ=255)
                                            tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc                        >  1:2664793/1‑69 (MQ=255)
                                            tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc                        >  1:340253/1‑69 (MQ=255)
                                            tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc                        >  1:1963554/1‑69 (MQ=255)
                                            tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc                        >  1:853196/1‑69 (MQ=255)
                                            tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc                        >  1:877865/1‑69 (MQ=255)
                                            tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc                        >  1:2978560/1‑69 (MQ=255)
                                            tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc                        >  1:2868515/1‑69 (MQ=255)
                                            tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc                        >  1:1003508/1‑69 (MQ=255)
                                                   aCGCAGGTCATGCCCCGGACGGTCAGCGACAAAGGTGATCTGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:438073/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGGCGGTCAGCGACAAAGGTGATCAGGTCACGATAGAGCGCCACGCCGTGCgg                 >  1:911101/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATa                                   >  1:1406410/1‑51 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:500377/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:2102754/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:662708/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:1337762/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:1385443/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:2080346/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:2547414/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:937040/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg                 >  1:2661973/1‑69 (MQ=255)
                                                   aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCAGTGCgg                 >  1:2096719/1‑69 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATGAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:471215/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:1154885/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:2376640/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:227826/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:3035889/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:1005814/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:2241891/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:715695/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:695067/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:1606346/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:556133/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:550712/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:3143759/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:1896876/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:166927/1‑67 (MQ=255)
                                                           cATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGtt           >  1:1585281/1‑67 (MQ=255)
                                                                  |                                                                    
CTGCGGCAGCCAGCCAAGTTCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGGCTTGTTCGGAGCCAG  >  minE/2425141‑2425275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A