Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,435,202 |
C→A |
40.6% |
I273I (ATC→ATA) |
gpp → |
guanosine pentaphosphatase/exopolyphosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,435,202 | 0 | C | A | 40.6%
| 54.8
/ 78.8
| 91 | I273I (ATC→ATA) | gpp | guanosine pentaphosphatase/exopolyphosphatase |
| Reads supporting (aligned to +/- strand): ref base C (11/43); new base A (13/24); total (24/67) |
| Fisher's exact test for biased strand distribution p-value = 1.48e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01 |
GCTGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGAAGGC > minE/2435151‑2435265
|
gCTGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGaa > 1:2097687/1‑67 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:90493/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:458483/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:3364216/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:3281738/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:2114437/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:2212132/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:2901282/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:2750174/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:2265379/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:2533991/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:2363664/69‑1 (MQ=255)
tGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGa < 1:1702594/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:274315/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:1966011/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:2407040/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:2498260/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:110935/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:2953313/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:2962792/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:3075162/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:999470/69‑1 (MQ=255)
gACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:1170271/69‑1 (MQ=255)
aCGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGaa < 1:3237225/68‑1 (MQ=255)
tGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTc < 1:3022588/69‑1 (MQ=255)
ggAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCa < 1:104897/69‑1 (MQ=255)
ggAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCa < 1:2755386/69‑1 (MQ=255)
aCGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:1915090/69‑1 (MQ=255)
aCGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:3425799/69‑1 (MQ=255)
aCGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:1876605/69‑1 (MQ=255)
aCGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:602025/69‑1 (MQ=255)
aCGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:224257/69‑1 (MQ=255)
cGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:2515295/68‑1 (MQ=255)
ttAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGAcc > 1:1828452/1‑69 (MQ=255)
aGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCAtttt > 1:2391960/1‑39 (MQ=255)
aGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCAtt > 1:2363312/1‑37 (MQ=255)
aGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGAccc > 1:1623242/1‑68 (MQ=255)
aGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGAccc > 1:1785928/1‑68 (MQ=255)
aGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCt > 1:2361211/1‑69 (MQ=255)
cccGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:658520/54‑1 (MQ=255)
cccGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:118079/54‑1 (MQ=255)
cccGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:553485/54‑1 (MQ=255)
cccGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:1328188/54‑1 (MQ=255)
cccGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:421939/54‑1 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:732092/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:1950760/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:276783/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:2916028/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:1855142/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:2748743/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:1460382/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:1334699/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:1177968/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:1988956/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:3085863/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggcgggcg > 1:3049413/1‑69 (MQ=255)
cGAGTGGTCTGGCGATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTggc > 1:2744338/1‑64 (MQ=255)
aGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTc < 1:2598850/46‑1 (MQ=255)
ggTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTgcg < 1:2522156/69‑1 (MQ=255)
cTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTg < 1:1905445/69‑1 (MQ=255)
gATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTTCGCTGCGTg < 1:1820154/68‑1 (MQ=255)
gATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTg < 1:1941641/68‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:355185/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:436003/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:462266/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:494927/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:55670/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:3340298/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:903323/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1188506/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1453556/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1712059/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1857639/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1884836/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:2099863/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:215377/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1760188/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1756518/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1547956/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:2539072/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:2618071/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:2672268/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:2702187/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:2751870/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1388690/69‑1 (MQ=255)
gATCCTGATAGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGa < 1:1215525/69‑1 (MQ=255)
cTGATCGCCATTTTTACCGAACTGAATATTCAgtg < 1:3222146/35‑1 (MQ=255)
cTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGAAGGc > 1:1742453/1‑69 (MQ=255)
cTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGAAGGc > 1:2785994/1‑69 (MQ=255)
cTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGAAGGc > 1:981233/1‑69 (MQ=255)
cTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGAAGGc > 1:997579/1‑69 (MQ=255)
|
GCTGACGCTGGAACGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAATATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGAAGGC > minE/2435151‑2435265
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
