Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,439,591 |
C→T |
75.1% |
E351E (GAG→GAA) |
ilvC ← |
ketol‑acid reductoisomerase, NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,439,591 | 0 | C | T | 75.1%
| 102.1
/ 31.0
| 88 | E351E (GAG→GAA) | ilvC | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (12/10); new base T (25/41); total (37/51) |
| Fisher's exact test for biased strand distribution p-value = 2.15e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 |
TCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCA > minE/2439523‑2439656
|
tCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCAT‑AGTACGCCTTTATCGAAGTACTCCTGCt > 1:454727/1‑69 (MQ=255)
cccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCa < 1:2303700/69‑1 (MQ=255)
cccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCa < 1:1714603/69‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:972887/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2349725/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2132531/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1973008/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2884817/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2908527/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:3372345/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1556060/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1507216/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1449830/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:861423/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:751126/67‑1 (MQ=255)
ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:430240/67‑1 (MQ=255)
ccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAt < 1:2205888/69‑1 (MQ=255)
ccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAt < 1:857752/69‑1 (MQ=255)
tgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2055025/67‑1 (MQ=255)
aTCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGcgcg < 1:729987/69‑1 (MQ=255)
aTCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGcgcg < 1:2823917/69‑1 (MQ=255)
aTCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGcgcg < 1:1271245/69‑1 (MQ=255)
catcaGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCaaa > 1:2022198/1‑69 (MQ=255)
catcaGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCaaa > 1:2738909/1‑69 (MQ=255)
catcaGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCaaa > 1:3108301/1‑69 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTcgcgct > 1:1215614/1‑57 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2831171/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2941171/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2723211/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2655557/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:3363486/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2424372/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2420361/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:705299/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:407626/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:1103788/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:1501191/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2338262/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:1058972/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:1345210/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:2407302/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:917150/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:2550862/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:446514/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:448255/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:3101044/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:837838/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:1332611/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:2741129/1‑60 (MQ=255)
aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:184368/1‑60 (MQ=255)
cGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGttt > 1:2193150/1‑69 (MQ=255)
ccTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGtttt < 1:480427/68‑1 (MQ=255)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:609458/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:553616/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:542605/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:843674/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:528570/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:847686/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:1321396/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:984077/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:414092/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:1576420/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2037832/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:159864/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:3346002/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:3272026/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:3150607/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2118455/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2732209/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:1740737/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2099218/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2625171/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2028245/36‑1 (MQ=37)
ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:1594587/36‑1 (MQ=37)
tATCTAAGTACTCCTGTTCACGGATTTTGCCTTCa < 1:756715/35‑1 (MQ=16)
tATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:959802/35‑1 (MQ=37)
aGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCtt < 1:673286/69‑1 (MQ=255)
aGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCtt < 1:2040964/69‑1 (MQ=255)
aGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCtt < 1:3150380/69‑1 (MQ=255)
aGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCAGTTTTGCCGGTCTCtt < 1:3366375/69‑1 (MQ=255)
aGTACTCCTGCTCGCAGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCtt < 1:1381634/69‑1 (MQ=255)
gTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTc > 1:14385/1‑69 (MQ=255)
gTACTCCTGCTCGCCGATTTTGCCTTCATACGGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTc > 1:18252/1‑69 (MQ=255)
cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:2032181/1‑37 (MQ=255)
cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCa > 1:1867630/1‑69 (MQ=255)
cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCa > 1:1251794/1‑69 (MQ=255)
cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCa > 1:2361823/1‑69 (MQ=255)
cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCAAGCCa > 1:1761087/1‑69 (MQ=255)
|
TCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCA > minE/2439523‑2439656
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
