Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,449,169 |
G→A |
29.3% |
R185H (CGC→CAC) |
yifB → |
predicted bifunctional protein, enzyme and transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,449,169 | 0 | G | A | 29.3%
| 79.4
/ 32.6
| 78 | R185H (CGC→CAC) | yifB | predicted bifunctional protein, enzyme and transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (26/29); new base A (18/5); total (44/34) |
| Fisher's exact test for biased strand distribution p-value = 1.35e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
TGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTA > minE/2449105‑2449225
|
tGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGccc > 1:2997321/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACAccc > 1:1152753/1‑65 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:1337024/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:2908961/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:2698910/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:3219560/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:3027641/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:201694/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:2035343/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:1279500/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:1231485/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:2187993/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:3096494/1‑68 (MQ=255)
cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:914199/1‑68 (MQ=255)
cTGATAACCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:1991/1‑68 (MQ=255)
gATAGCCGATCATCTGCATGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:2002059/69‑1 (MQ=255)
gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:263278/69‑1 (MQ=255)
gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:481784/69‑1 (MQ=255)
gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:3292829/69‑1 (MQ=255)
gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:2816202/69‑1 (MQ=255)
gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:3062869/69‑1 (MQ=255)
gCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACt > 1:3370659/1‑69 (MQ=255)
gCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACt > 1:2115206/1‑69 (MQ=255)
cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTg > 1:17899/1‑68 (MQ=255)
cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:2918755/1‑69 (MQ=255)
cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:1980186/1‑69 (MQ=255)
cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:674143/1‑69 (MQ=255)
cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:2586502/1‑69 (MQ=255)
cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:2251165/1‑69 (MQ=255)
atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:2537010/69‑1 (MQ=255)
atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:2928382/69‑1 (MQ=255)
atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:3057307/69‑1 (MQ=255)
atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:1299031/69‑1 (MQ=255)
atcTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTATCGAACGCCCGAAACCAACTGATGCAg < 1:3109224/69‑1 (MQ=255)
tcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:1299442/68‑1 (MQ=255)
cAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATcc > 1:2246788/1‑69 (MQ=255)
tgtgCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:2669019/44‑1 (MQ=255)
tgtgCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGgcgc < 1:2165061/67‑1 (MQ=255)
tgtgCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGgcgc < 1:1473579/67‑1 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCTAACGCCCGAAACCAACTGATGCAg < 1:2078615/51‑1 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGaaa > 1:2569504/1‑38 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAATCCAACTGATGCAg < 1:2858125/51‑1 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:2497355/51‑1 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg > 1:3230825/1‑51 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:2663206/51‑1 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:412390/51‑1 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg > 1:590504/1‑51 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg > 1:1631100/1‑51 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:1609168/51‑1 (MQ=255)
cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:612463/51‑1 (MQ=255)
tCTGGAAGTTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACAACATGa > 1:3361057/1‑69 (MQ=255)
tCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACAACATGa > 1:3177122/1‑69 (MQ=255)
tCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACAACATGa > 1:1228663/1‑69 (MQ=255)
tCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACAACATGa > 1:1494341/1‑69 (MQ=255)
ggTAAGCACGCTCTCGAACGCCCGATACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:665338/69‑1 (MQ=255)
ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:1906360/69‑1 (MQ=255)
ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:3194067/69‑1 (MQ=255)
ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:1160450/69‑1 (MQ=255)
ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:11116/69‑1 (MQ=255)
ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATACCGGGCGCTACAACATGATCTCAGt < 1:2326511/69‑1 (MQ=255)
ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGACGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:1222405/69‑1 (MQ=255)
ggTAAGCACGCTCTCGAACGCCCGAAAACAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:2556804/69‑1 (MQ=255)
gTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:1788888/68‑1 (MQ=255)
tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:1840727/1‑69 (MQ=255)
tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:1483174/1‑69 (MQ=255)
tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:818564/1‑69 (MQ=255)
tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:879272/1‑69 (MQ=255)
tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:96538/1‑69 (MQ=255)
aaGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGAt < 1:2368894/69‑1 (MQ=255)
cACGCTCTCGAACGCCCGAACCCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:2658903/1‑69 (MQ=255)
cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGgcgc > 1:121899/1‑46 (MQ=255)
cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:3059592/1‑69 (MQ=255)
cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:2937750/1‑69 (MQ=255)
cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:2570388/1‑69 (MQ=255)
cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:1396788/1‑69 (MQ=255)
cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCAGGGCGCTAAAACATGATCTCAGTGATGtt > 1:1782639/1‑69 (MQ=255)
aCGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTa < 1:1982651/69‑1 (MQ=255)
aCGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTa < 1:1566981/69‑1 (MQ=255)
|
TGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTA > minE/2449105‑2449225
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
