Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,505,793 |
A→G |
45.0% |
V83V (GTA→GTG) |
yicJ → |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,505,793 | 0 | A | G | 45.0%
| 11.2
/ 65.7
| 80 | V83V (GTA→GTG) | yicJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (8/36); new base G (17/19); total (25/55) |
| Fisher's exact test for biased strand distribution p-value = 7.54e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCGATCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAG > minE/2505725‑2505855
|
tCCGATCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTa > 1:1169619/1‑69 (MQ=255)
cGATCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCt > 1:2695105/1‑69 (MQ=255)
cGATCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCt > 1:3195905/1‑69 (MQ=255)
aTCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTg < 1:573449/68‑1 (MQ=255)
aTCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTg < 1:1643904/68‑1 (MQ=255)
aTCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTg < 1:1132447/68‑1 (MQ=255)
aTCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTg < 1:1147256/68‑1 (MQ=255)
aTCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTg < 1:3162068/68‑1 (MQ=255)
aTCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTg < 1:1389874/68‑1 (MQ=255)
aTCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTg < 1:1436174/68‑1 (MQ=255)
ccTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTg < 1:379648/66‑1 (MQ=255)
tGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCa < 1:283863/68‑1 (MQ=255)
tGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCa < 1:2398177/68‑1 (MQ=255)
tGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCa < 1:749989/68‑1 (MQ=255)
tGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCa < 1:112376/68‑1 (MQ=255)
ttgttgGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCa > 1:1536908/1‑69 (MQ=255)
ccGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCggg < 1:2419671/68‑1 (MQ=255)
ccGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCggg < 1:891462/68‑1 (MQ=255)
ccGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCggg < 1:1359924/68‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:1037932/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:2416915/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:2534627/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:3156977/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:3371449/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:2120763/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:2823034/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:1885993/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:1841407/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:1844615/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:594125/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:600748/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:1067643/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:639460/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:713797/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:94988/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:906794/46‑1 (MQ=255)
tCGAACGCGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:782176/46‑1 (MQ=255)
tCGAACACGCTCTCGCTGGGGTAAATTTCGGCCGTGGGTGCTGttt < 1:1871797/46‑1 (MQ=255)
cGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGtg > 1:1384219/1‑68 (MQ=255)
cGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTc > 1:1863231/1‑69 (MQ=255)
cGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTc > 1:252201/1‑69 (MQ=255)
cGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTc > 1:2554788/1‑69 (MQ=255)
cgcgCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCtgtg < 1:1047890/69‑1 (MQ=255)
cgcgCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCtgtg < 1:2776921/69‑1 (MQ=255)
cgcgCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCtgtg < 1:1432349/69‑1 (MQ=255)
cgcgCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCtgtg < 1:1489618/69‑1 (MQ=255)
cgcgCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCtgtg < 1:3240022/69‑1 (MQ=255)
gCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCTGGATCGTCTGTGTa < 1:1623383/62‑1 (MQ=255)
gCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTa < 1:2882624/62‑1 (MQ=255)
gCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTa < 1:2466288/62‑1 (MQ=255)
gCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTa < 1:139163/62‑1 (MQ=255)
gCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTa < 1:2394496/62‑1 (MQ=255)
gCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTa < 1:1457860/62‑1 (MQ=255)
aaTTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCtat > 1:1748819/1‑62 (MQ=255)
aaTTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:1339423/1‑67 (MQ=255)
aaTTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:2298224/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGa > 1:293600/1‑42 (MQ=38)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACt > 1:1548129/1‑55 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:2890025/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:1092328/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:1387816/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:749/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:645302/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:1588477/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:1969684/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:3383660/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:2301332/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:32089/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:2319701/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:2663519/1‑67 (MQ=255)
aaTTTCGGCCGTGGGTGCTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCAc > 1:2796414/1‑67 (MQ=255)
tttCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGc < 1:370518/67‑1 (MQ=255)
tttCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGc < 1:1881210/67‑1 (MQ=255)
tttCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGc < 1:367914/67‑1 (MQ=255)
tttCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGc < 1:337526/67‑1 (MQ=255)
tttCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGc < 1:781555/67‑1 (MQ=255)
tttCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGc < 1:804154/67‑1 (MQ=255)
tttCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGc < 1:3117307/67‑1 (MQ=255)
tttCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGc < 1:2972322/67‑1 (MQ=255)
tttCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGc < 1:986886/67‑1 (MQ=255)
tGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAg < 1:1714236/68‑1 (MQ=255)
|
TCCGATCCTTGCATGGGGTTGTTGGCCGATCGAACGCGCTCTCGCTGGGGTAAATTTCGTCCGTGGGTACTGTTTGGCGCACTGCCATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAG > minE/2505725‑2505855
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
