Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,555,850 |
G→A |
31.6% |
G122G (GGC→GGT) |
gpmI ← |
phosphoglycero mutase III, cofactor‑independent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,555,850 | 0 | G | A | 31.6%
| 72.7
/ 39.0
| 82 | G122G (GGC→GGT) | gpmI | phosphoglycero mutase III, cofactor‑independent |
| Reads supporting (aligned to +/- strand): ref base G (42/14); new base A (16/10); total (58/24) |
| Fisher's exact test for biased strand distribution p-value = 2.97e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01 |
CAGCTGCCAGTTCTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGC > minE/2555798‑2555912
|
cAGCTGCCAGTTCTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGa > 1:2887250/1‑68 (MQ=255)
cAGCTGCCAGTTCTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGa > 1:2125340/1‑68 (MQ=255)
tCTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCa > 1:926177/1‑61 (MQ=255)
tCTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATgtgt > 1:2790440/1‑69 (MQ=255)
tCTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATgtgt > 1:922227/1‑69 (MQ=255)
cTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGCCCCATAATGTGTa > 1:478501/1‑69 (MQ=255)
cTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt < 1:540663/61‑1 (MQ=255)
cTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt < 1:136349/61‑1 (MQ=255)
cTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt < 1:2566612/61‑1 (MQ=255)
cTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt < 1:525777/61‑1 (MQ=255)
cTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt < 1:3105184/61‑1 (MQ=255)
cTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt < 1:315713/61‑1 (MQ=255)
cTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAAt > 1:2504812/1‑64 (MQ=255)
cTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTa > 1:1486650/1‑69 (MQ=255)
tACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt < 1:454740/60‑1 (MQ=255)
aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:2269024/1‑69 (MQ=255)
aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:2474193/1‑69 (MQ=255)
aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:912633/1‑69 (MQ=255)
aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:822425/1‑69 (MQ=255)
aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:1601251/1‑69 (MQ=255)
aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:2557456/1‑69 (MQ=255)
aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:1251710/1‑69 (MQ=255)
gCCATGATGTGATCTTCGTGGCTGTGTACGCCGCGTGCGGAGAGCAGACCCAt > 1:2819594/1‑53 (MQ=255)
gCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt > 1:1728944/1‑53 (MQ=255)
gCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt > 1:1907522/1‑53 (MQ=255)
gCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt > 1:956432/1‑53 (MQ=255)
gCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt > 1:2755628/1‑53 (MQ=255)
gCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCAt > 1:1432991/1‑53 (MQ=255)
gCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTg > 1:3425106/1‑69 (MQ=255)
gCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTg > 1:2132892/1‑69 (MQ=255)
atgTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCg > 1:984221/1‑69 (MQ=255)
atgTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCg > 1:1435065/1‑69 (MQ=255)
atgTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCg > 1:75220/1‑69 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCATACCCATAATGTGTACTGCttt < 1:3203661/62‑1 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:2560981/62‑1 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:37967/62‑1 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1057093/62‑1 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:2333540/62‑1 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1718493/62‑1 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1488432/62‑1 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1479397/62‑1 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1208095/62‑1 (MQ=255)
atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1033131/62‑1 (MQ=255)
atgTGATCATCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCg > 1:1836677/1‑69 (MQ=255)
tgtgATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGt > 1:1987068/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTa > 1:741422/1‑49 (MQ=39)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:835176/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:1237611/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:814089/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:507142/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:3395778/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:3223933/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:3176310/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:1699349/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:2941956/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:2326685/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:1918919/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:2893992/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:2789204/1‑69 (MQ=255)
tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:289063/1‑69 (MQ=255)
ttCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc > 1:1714229/1‑69 (MQ=255)
tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:1616458/65‑1 (MQ=255)
tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:668923/65‑1 (MQ=255)
tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:1317605/65‑1 (MQ=255)
tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:2005982/65‑1 (MQ=255)
tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:1221931/65‑1 (MQ=255)
tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:1211206/65‑1 (MQ=255)
tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:2490204/65‑1 (MQ=255)
tACGTCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:454535/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGttt > 1:2128197/1‑52 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGttt > 1:99796/1‑52 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:1350300/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:682528/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:1879360/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:748011/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:444996/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:2281709/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:2467966/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:3259122/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:2904147/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:1188843/1‑69 (MQ=255)
tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:177931/1‑69 (MQ=255)
cgccgcCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTAt > 1:1569668/1‑60 (MQ=255)
cgccgcCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:3147040/1‑67 (MQ=255)
cgccgcCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcg > 1:438403/1‑66 (MQ=255)
|
CAGCTGCCAGTTCTACCATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGC > minE/2555798‑2555912
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
