Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,591,021 |
G→A |
47.0% |
A24A (GCG→GCA) |
hdeA → |
stress response protein acid‑resistance protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,591,021 | 0 | G | A | 47.0%
| 9.8
/ 69.2
| 84 | A24A (GCG→GCA) | hdeA | stress response protein acid‑resistance protein |
| Reads supporting (aligned to +/- strand): ref base G (19/25); new base A (21/18); total (40/44) |
| Fisher's exact test for biased strand distribution p-value = 3.83e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 |
AAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGATTTCCTGGCTGTGGACG > minE/2590957‑2591088
|
aaGTATTAGGCGTTATTCTTGGTGTTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:353442/69‑1 (MQ=255)
aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTGTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:673046/69‑1 (MQ=255)
aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:1026971/69‑1 (MQ=255)
aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:2060144/69‑1 (MQ=255)
aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:1987403/69‑1 (MQ=255)
aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:2903176/69‑1 (MQ=255)
aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:313558/69‑1 (MQ=255)
aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:575666/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCTCaaaa < 1:3017012/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1693330/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:2461095/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:2654817/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:2823466/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:2841692/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:2844800/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:821940/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:766539/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1376486/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1652952/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:736031/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1254205/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1284357/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:629653/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1447452/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:639297/69‑1 (MQ=255)
aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:672743/69‑1 (MQ=255)
gTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:984325/68‑1 (MQ=255)
ttAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAagca > 1:284852/1‑69 (MQ=255)
aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATaa > 1:1211019/1‑68 (MQ=255)
aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATaa > 1:2216069/1‑68 (MQ=255)
aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATaa > 1:1411392/1‑68 (MQ=255)
aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATaa > 1:289044/1‑68 (MQ=255)
cTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:952910/69‑1 (MQ=255)
cTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:495866/69‑1 (MQ=255)
cTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:247092/69‑1 (MQ=255)
cTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:3228198/69‑1 (MQ=255)
gCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:491841/67‑1 (MQ=255)
cttcttCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAAc > 1:1042619/1‑69 (MQ=255)
cttcttCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAAc > 1:668183/1‑69 (MQ=255)
cttcttCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAAc > 1:1556102/1‑69 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2896481/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2608252/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:3271568/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:1617078/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2383094/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2431618/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:458694/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:843540/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2057454/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:920692/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:924728/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2031931/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:603186/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2136088/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2060678/1‑47 (MQ=255)
cttctGCCAGTAGTGAGCAATGCAGCGGAAGCACAAAAAGCAGCTGa > 1:2140641/1‑47 (MQ=255)
ttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:2730912/1‑63 (MQ=255)
ttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:723856/1‑63 (MQ=255)
ttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:1072789/1‑63 (MQ=255)
ttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:2038352/1‑63 (MQ=255)
ttctGCCAGTAGTGAGCAATGCAGAGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:1389426/1‑63 (MQ=255)
gtgAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGaa < 1:10809/69‑1 (MQ=255)
gtgAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGaa < 1:3039422/69‑1 (MQ=255)
gtgAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGaa < 1:2908252/69‑1 (MQ=255)
gAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAg < 1:69790/68‑1 (MQ=255)
gAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAg < 1:2735714/68‑1 (MQ=255)
gAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAg < 1:454453/68‑1 (MQ=255)
gAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAg < 1:3430298/68‑1 (MQ=255)
gAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAg < 1:3272051/68‑1 (MQ=255)
gAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAg < 1:1882285/68‑1 (MQ=255)
gAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAg < 1:3080646/68‑1 (MQ=255)
gAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAg < 1:1846202/68‑1 (MQ=255)
aGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAg < 1:433781/67‑1 (MQ=255)
cAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGAttt > 1:2092634/1‑69 (MQ=255)
aaTGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGATTTc > 1:687258/1‑69 (MQ=255)
aaTGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGATTTc > 1:2658297/1‑69 (MQ=255)
aaTGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGATTTc > 1:1564223/1‑69 (MQ=255)
gCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAGCCGGTCAACTCCTGGACCTGTGAAGATTTCCTg > 1:114008/1‑69 (MQ=255)
gCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAGCCGGTCAACTCCTGGACCTGTGAAGATTTCCTg > 1:2739694/1‑69 (MQ=255)
gCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAGCCGGTCAACTCCTGGACCTGTGAAGATTTCCTg > 1:1995971/1‑69 (MQ=255)
gCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAGCCGGTCAACTCCTGGACCTGTGAAGATTTCCTg > 1:1649713/1‑69 (MQ=255)
gCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAGCCGGTCAACTCCTGGACCTGTGAAGATTTCCTg > 1:2188594/1‑69 (MQ=255)
gCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGATTTCCTg > 1:781299/1‑69 (MQ=255)
gATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGATTTCCTGGCtgtg > 1:3171141/1‑69 (MQ=255)
cgcAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGATTTCCTGGCTGTGGACg > 1:762039/1‑69 (MQ=255)
cgcAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGATTTCCTGGCTGTGGACg > 1:2581780/1‑69 (MQ=255)
|
AAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAACTCCTGGACCTGTGAAGATTTCCTGGCTGTGGACG > minE/2590957‑2591088
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
