Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,601,306 |
T→C |
26.1% |
F319F (TTT→TTC) |
prlC → |
oligopeptidase A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,601,306 | 0 | T | C | 26.1%
| 90.5
/ 29.4
| 80 | F319F (TTT→TTC) | prlC | oligopeptidase A |
| Reads supporting (aligned to +/- strand): ref base T (33/26); new base C (13/8); total (46/34) |
| Fisher's exact test for biased strand distribution p-value = 7.98e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAA > minE/2601257‑2601373
|
aaCGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGcc > 1:2930834/1‑59 (MQ=255)
aaCGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGc > 1:1741162/1‑68 (MQ=255)
aaCGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCg > 1:3038803/1‑69 (MQ=255)
aaCGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCg > 1:1923010/1‑69 (MQ=255)
aaCGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCg > 1:1737348/1‑69 (MQ=255)
aaCGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCg > 1:1495333/1‑69 (MQ=255)
aaCGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCg > 1:1576752/1‑69 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:2532667/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:1902116/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:1860600/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:1936675/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:1594426/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:1498728/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:2271193/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:1465289/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:2290197/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:3380735/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:2424474/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:1231073/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:781293/63‑1 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGc > 1:1653543/1‑69 (MQ=255)
cacaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGc > 1:454502/1‑69 (MQ=255)
acaAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATg < 1:1568385/62‑1 (MQ=255)
caAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCa < 1:2661254/68‑1 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAAt > 1:3063654/1‑40 (MQ=25)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGat > 1:1662047/1‑69 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGat > 1:274868/1‑69 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGat > 1:2035587/1‑69 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGat > 1:3214155/1‑69 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGat > 1:3300673/1‑69 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGat > 1:232975/1‑69 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGat > 1:2672402/1‑69 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGat > 1:250201/1‑69 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGat > 1:838419/1‑69 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGa > 1:2821841/1‑68 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGa > 1:2216230/1‑68 (MQ=255)
aaaGAGCTGGCGCAACTGCGCGCATTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTTGGa > 1:3059478/1‑68 (MQ=255)
cTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCt < 1:489821/69‑1 (MQ=255)
cTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCt < 1:932074/69‑1 (MQ=255)
tGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCt < 1:1681689/68‑1 (MQ=255)
tGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCt < 1:1462500/68‑1 (MQ=255)
tGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCt < 1:2809880/68‑1 (MQ=255)
gcgcAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCt < 1:1391701/66‑1 (MQ=255)
gcAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTtac < 1:2881638/67‑1 (MQ=255)
cAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTtac < 1:1491606/66‑1 (MQ=255)
aTTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTtagtacg > 1:2726749/1‑67 (MQ=255)
aTTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACa > 1:571097/1‑68 (MQ=255)
aTTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACa > 1:2604637/1‑68 (MQ=255)
aTTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACa > 1:2934895/1‑68 (MQ=255)
aTTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACa > 1:292138/1‑68 (MQ=255)
aTTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACa > 1:1996788/1‑68 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAg > 1:3092107/1‑38 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGt > 1:1058318/1‑48 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTgg > 1:3135559/1‑50 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTgg > 1:596238/1‑50 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTgg > 1:1366911/1‑50 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTgg > 1:630278/1‑50 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTgg > 1:1371854/1‑50 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTgg > 1:633967/1‑50 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTgg > 1:704475/1‑50 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTgg > 1:860827/1‑50 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTg > 1:2949637/1‑49 (MQ=255)
tGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTg > 1:2893971/1‑49 (MQ=255)
gcgcgcCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa < 1:324720/69‑1 (MQ=255)
gcgcgcCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa < 1:2533173/69‑1 (MQ=255)
gcgcgcCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa < 1:1125375/69‑1 (MQ=255)
gcgcgcCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa < 1:775103/69‑1 (MQ=255)
gcgcgcCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa < 1:727847/69‑1 (MQ=255)
gcgcgcCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa < 1:198917/69‑1 (MQ=255)
gcgcgcCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa < 1:2859678/69‑1 (MQ=255)
gcgcgcCTTCGCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa < 1:618874/69‑1 (MQ=255)
gCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAg > 1:266079/1‑37 (MQ=255)
gCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGt < 1:2393132/47‑1 (MQ=255)
gCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGt < 1:1878936/47‑1 (MQ=255)
gCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTgg < 1:370390/49‑1 (MQ=255)
gCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCg > 1:606346/1‑68 (MQ=255)
gCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCg > 1:2710773/1‑68 (MQ=255)
gCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCg > 1:178467/1‑68 (MQ=255)
gCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCg > 1:3287138/1‑68 (MQ=255)
gCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCg > 1:3125840/1‑68 (MQ=255)
ttGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGaaaaacaaa < 1:1986448/69‑1 (MQ=255)
ttGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGaaaaacaaa < 1:1219168/69‑1 (MQ=255)
|
AACGCGCGCGTCCACAAGGCGAAAAAGAGCTGGCGCAATTGCGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAA > minE/2601257‑2601373
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
