Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,617,694 |
A→C |
31.7% |
G93G (GGT→GGG) |
yhhQ ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,617,694 | 0 | A | C | 31.7%
| 84.5
/ 74.7
| 101 | G93G (GGT→GGG) | yhhQ | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (23/46); new base C (17/15); total (40/61) |
| Fisher's exact test for biased strand distribution p-value = 8.02e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01 |
Only 100 of 103 total aligned reads displayed.
TGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGATAA > minE/2617630‑2617761
|
tGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCa < 1:1069378/69‑1 (MQ=255)
tGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCa < 1:3295755/69‑1 (MQ=255)
tGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCa < 1:3241249/69‑1 (MQ=255)
ggTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCata < 1:232170/67‑1 (MQ=255)
gTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAg < 1:1020359/69‑1 (MQ=255)
gTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAg < 1:1676501/69‑1 (MQ=255)
gTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAg < 1:3219115/69‑1 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2758470/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2455131/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2950630/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2223850/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:376334/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2060895/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:1953119/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:3208974/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:585023/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2981795/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:1506173/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:135644/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:1353402/62‑2 (MQ=255)
tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:1209263/62‑2 (MQ=255)
gctaCAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:300215/66‑1 (MQ=255)
gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:642899/69‑1 (MQ=255)
gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:2905312/69‑1 (MQ=255)
gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:33337/69‑1 (MQ=255)
gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:2367590/69‑1 (MQ=255)
gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:35063/69‑1 (MQ=255)
cGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:1229325/68‑1 (MQ=255)
cGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:536454/68‑1 (MQ=255)
gTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:1143512/69‑1 (MQ=255)
gTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:1664173/69‑1 (MQ=255)
gTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:2249286/69‑1 (MQ=255)
gAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGc < 1:703543/49‑1 (MQ=255)
gAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGc < 1:684833/49‑1 (MQ=255)
gAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGc < 1:58399/49‑1 (MQ=255)
gAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGc < 1:2360554/49‑1 (MQ=255)
tGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:2590432/62‑1 (MQ=255)
ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGCATAGCGACGAGATGACGTAGga < 1:3142348/60‑1 (MQ=255)
ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:171409/60‑1 (MQ=255)
ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:3376493/60‑1 (MQ=255)
ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:450812/60‑1 (MQ=255)
ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:3090544/60‑1 (MQ=255)
ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:2974658/60‑1 (MQ=255)
ggCGACTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:2453329/60‑1 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:3153321/54‑1 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:82201/1‑67 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:2594126/1‑67 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:617806/1‑67 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:3072595/1‑67 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:612177/1‑67 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:2231138/1‑67 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:1474799/1‑67 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:1992714/1‑67 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAgg > 1:2576192/1‑69 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAgg > 1:1253656/1‑69 (MQ=255)
tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAgg > 1:1979635/1‑69 (MQ=255)
ccGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGAt < 1:2111666/68‑1 (MQ=255)
ccGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGAt < 1:2829793/68‑1 (MQ=255)
ccGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGAt < 1:1598137/68‑1 (MQ=255)
ccGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGAt < 1:1236117/68‑1 (MQ=255)
aaTCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAtt < 1:1255979/69‑1 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGGAATGCGGGGATCa > 1:1429148/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATc > 1:310434/1‑64 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:325766/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:3372276/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:10266/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:678474/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:858748/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:3206630/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1072707/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1110254/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1128437/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:2705635/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1146064/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:2520323/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1190698/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1891244/1‑65 (MQ=255)
tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:190258/1‑65 (MQ=255)
tCCCTGCCAGGAACCCATATAGAATAGCGACgaga > 1:1457972/1‑35 (MQ=255)
tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTa > 1:2442406/1‑68 (MQ=255)
tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:2840864/1‑69 (MQ=255)
tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:1629162/1‑69 (MQ=255)
tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:165292/1‑69 (MQ=255)
tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:3204965/1‑69 (MQ=255)
tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:2169768/1‑69 (MQ=255)
tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:3316298/1‑69 (MQ=255)
ccTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAt > 1:533646/1‑64 (MQ=255)
ccTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAt > 1:702083/1‑64 (MQ=255)
ccTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAt > 1:2421347/1‑64 (MQ=255)
ccTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAt > 1:356670/1‑64 (MQ=255)
aGGAACCCATATAGAATATCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:614695/69‑1 (MQ=255)
aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATCACGCAGGGATCATTACCGCGAAGa < 1:3235735/69‑1 (MQ=255)
aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:1562420/69‑1 (MQ=255)
aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:628811/69‑1 (MQ=255)
aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:2431780/69‑1 (MQ=255)
aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:2576512/69‑1 (MQ=255)
aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:2789802/69‑1 (MQ=255)
ggAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:2178731/68‑1 (MQ=255)
aaCCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGATaa < 1:845478/69‑1 (MQ=255)
aaCCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGATaa < 1:2025757/69‑1 (MQ=255)
|
TGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGATAA > minE/2617630‑2617761
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
