Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,619,848 |
T→C |
23.6% |
A297A (GCA→GCG) |
zntA ← |
zinc, cobalt and lead efflux system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,619,848 | 0 | T | C | 23.6%
| 94.1
/ 23.9
| 76 | A297A (GCA→GCG) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base T (29/29); new base C (5/13); total (34/42) |
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 |
AGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGAGAGCA > minE/2619786‑2619915
|
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgggc > 1:1255469/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:1701686/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:1944671/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:1936985/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:40934/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:350950/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:1250536/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:2911044/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:1443920/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:2565282/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcgc > 1:3356719/1‑69 (MQ=255)
aGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTgcgcg > 1:743321/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCGCGTTGCGCGCTCCAc > 1:1790555/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCAc > 1:3348802/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCAc > 1:898804/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCAc > 1:1750718/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCAc > 1:148148/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCAc > 1:3417435/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCAc > 1:854604/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCAc > 1:291204/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCAc > 1:2578504/1‑68 (MQ=255)
tCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTT‑CGCGCTCCAcc > 1:3214203/1‑68 (MQ=255)
aaCGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCgg < 1:1528751/68‑1 (MQ=255)
aaCGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCgg < 1:676952/68‑1 (MQ=255)
aaCGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCgg < 1:548657/68‑1 (MQ=255)
cACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGa < 1:1992614/69‑1 (MQ=255)
cACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGa < 1:1858112/69‑1 (MQ=255)
cACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGa < 1:1076221/69‑1 (MQ=255)
aCCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCAGTTGCGCGCTCCACCgg < 1:1940958/62‑1 (MQ=255)
aGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCgg < 1:824394/59‑1 (MQ=255)
tCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTc < 1:2435162/69‑1 (MQ=255)
tCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTc < 1:1068257/69‑1 (MQ=255)
tCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTc < 1:689045/69‑1 (MQ=255)
tCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTc < 1:2913402/69‑1 (MQ=255)
tCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTc < 1:319047/69‑1 (MQ=255)
tCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTc < 1:3364528/69‑1 (MQ=255)
tCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTc < 1:501203/69‑1 (MQ=255)
tCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTc < 1:1574109/69‑1 (MQ=255)
tCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCGCCACCGGAATGGATTCGCCGGTc < 1:3148425/69‑1 (MQ=255)
cTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTc < 1:1167199/68‑1 (MQ=255)
agcagGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCaaa < 1:1534129/69‑1 (MQ=255)
gcagGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTtc > 1:2040703/1‑62 (MQ=255)
gcagGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTtc > 1:1987590/1‑62 (MQ=255)
gcagGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTtc > 1:2844619/1‑62 (MQ=255)
gcagGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTtc > 1:972884/1‑62 (MQ=255)
gcagGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCaaa < 1:2707471/68‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:2370303/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:553/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:44217/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:1127068/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:1423827/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:1497406/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:3240369/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:1747799/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:2722409/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:2156101/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:797352/53‑1 (MQ=255)
gaaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:2428265/53‑1 (MQ=255)
aaCTTTATCGCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACt < 1:623287/53‑1 (MQ=255)
ttATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCaaa > 1:171027/1‑59 (MQ=255)
ttATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCaaa > 1:1971098/1‑59 (MQ=255)
ttATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCaaa > 1:1117767/1‑59 (MQ=255)
gCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACTTTCATCAAAACTGGCAAACGGt > 1:193431/1‑67 (MQ=255)
gCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACTTTCATCAAAACTGGCAAACGGt > 1:2901632/1‑67 (MQ=255)
gCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACTTTCATCAAAACTGGCAAACGGt > 1:1485231/1‑67 (MQ=255)
gCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACTTTCATCAAAACTGGCAAACGGt > 1:2567298/1‑67 (MQ=255)
gCCCGTCGCGCGCTCCACAGGAATGGATTCTCCGGTCAGGGCACTTTCATCAAAACTGGCAAACGGt > 1:378190/1‑67 (MQ=255)
cGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGagag < 1:88372/69‑1 (MQ=255)
cGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGagag < 1:2562924/69‑1 (MQ=255)
cGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGagag < 1:430527/69‑1 (MQ=255)
cGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGagag < 1:2902593/69‑1 (MQ=255)
cGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGagag < 1:2616733/69‑1 (MQ=255)
cGTTGCGCGCTCCACCGGAATGGATTCGCAGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGagag < 1:1515002/69‑1 (MQ=255)
gTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCGAACGGTGagag < 1:995350/68‑1 (MQ=255)
gTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGagag < 1:500317/68‑1 (MQ=255)
gTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGagag < 1:1551781/68‑1 (MQ=255)
ttGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGAGAGCa < 1:1061026/69‑1 (MQ=255)
ttGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGAGAGCa < 1:2530106/69‑1 (MQ=255)
ttGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGAGAGCa < 1:1654969/69‑1 (MQ=255)
ttGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGAGAGCa < 1:1653409/69‑1 (MQ=255)
ttGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGAGAGCa < 1:1512228/69‑1 (MQ=255)
|
AGCACTTCCAACGTCACCAGACGGTCTACGCTGGTGGCACCAGCAGGGACTTTATCGCCCGTTGCGCGCTCCACCGGAATGGATTCGCCGGTCAGGGCGCTTTCATCAAAACTGGCAAACGGTGAGAGCA > minE/2619786‑2619915
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
