Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,623,632 |
G→A |
48.0% |
E229E (GAG→GAA) |
ftsY → |
fused Signal Recognition Particle (SRP) receptor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,623,632 | 0 | G | A | 48.0%
| 10.0
/ 89.2
| 102 | E229E (GAG→GAA) | ftsY | fused Signal Recognition Particle (SRP) receptor |
| Reads supporting (aligned to +/- strand): ref base G (31/22); new base A (28/21); total (59/43) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 103 total aligned reads displayed.
AGAAAATCTCGGTTCCGGATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACC > minE/2623566‑2623695
|
aGAAAATCTCGGTTCCGGATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTgagga < 1:2294736/69‑1 (MQ=255)
ccGGATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAag < 1:837343/63‑1 (MQ=255)
ccGGATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAag < 1:355461/63‑1 (MQ=255)
ccGGATTCATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAag < 1:3119025/63‑1 (MQ=255)
cGGATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAag < 1:78789/62‑1 (MQ=255)
cGGATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAag < 1:1561907/62‑1 (MQ=255)
tttATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTg < 1:2100042/69‑1 (MQ=255)
tttATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTg < 1:3033761/69‑1 (MQ=255)
tttATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTg < 1:2894371/69‑1 (MQ=255)
tttATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTg < 1:2293831/69‑1 (MQ=255)
tttATCAGCCTGTGCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTg < 1:1671254/69‑1 (MQ=255)
tATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAag < 1:420540/56‑1 (MQ=255)
aGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGcc < 1:121917/69‑1 (MQ=255)
aGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGcc < 1:463969/69‑1 (MQ=255)
aGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGcc < 1:156624/69‑1 (MQ=255)
cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:2002535/1‑69 (MQ=255)
cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:2788213/1‑69 (MQ=255)
cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:1438208/1‑69 (MQ=255)
cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:590117/1‑69 (MQ=255)
cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:1789734/1‑69 (MQ=255)
tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGAtgtg > 1:3341415/1‑68 (MQ=255)
tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTgg > 1:3061184/1‑69 (MQ=255)
tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTgg > 1:437462/1‑69 (MQ=255)
tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTgg > 1:1595835/1‑69 (MQ=255)
tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTgg > 1:2928785/1‑69 (MQ=255)
aaaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:2845015/1‑53 (MQ=255)
aaaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:1968045/1‑53 (MQ=255)
aaaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:225417/1‑53 (MQ=255)
aaaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:1214477/1‑53 (MQ=255)
aaaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:842562/1‑53 (MQ=255)
aaaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:2462622/1‑53 (MQ=255)
aaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:2830007/1‑52 (MQ=255)
aaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGaa > 1:2977405/1‑68 (MQ=255)
aaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATAGCCGATGTGGGTGTGGa > 1:1640572/1‑67 (MQ=255)
aaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACca < 1:1973201/69‑1 (MQ=255)
aaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACca < 1:236337/69‑1 (MQ=255)
aaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACca < 1:147726/69‑1 (MQ=255)
aaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACca < 1:957601/69‑1 (MQ=255)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:2475052/1‑43 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2845571/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:286605/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2937253/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2993965/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2999580/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2678697/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:3223331/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:524887/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:530891/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:988592/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1258448/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1249620/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1642928/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1249970/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2141025/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1155659/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1569402/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2000469/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2072459/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1186333/1‑48 (MQ=37)
tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCC‑ATGTgggc > 1:1565781/1‑47 (MQ=21)
gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTTGGTGTGGAAACCACACGTaa > 1:1082915/1‑65 (MQ=255)
gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTaa > 1:1304813/1‑65 (MQ=255)
gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTaa > 1:1681585/1‑65 (MQ=255)
gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTaa > 1:2748434/1‑65 (MQ=255)
gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTaa > 1:357903/1‑65 (MQ=255)
gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTaa > 1:2340394/1‑65 (MQ=255)
gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAAtt < 1:2183212/69‑1 (MQ=255)
gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAAtt < 1:110895/69‑1 (MQ=255)
gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAAt > 1:2735477/1‑68 (MQ=255)
atCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGcc < 1:1003905/38‑1 (MQ=255)
tttGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACca < 1:2912659/52‑1 (MQ=255)
tttGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCAcc > 1:1635859/1‑69 (MQ=255)
tttGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCAcc > 1:1742845/1‑69 (MQ=255)
tttGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCAcc > 1:1158165/1‑69 (MQ=255)
tttGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCAcc > 1:2626072/1‑69 (MQ=255)
tttGAAGAGCTGGAAGAACATCTGTTGATCGCCGATGTgg < 1:5108/40‑1 (MQ=21)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2342942/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:3413363/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:3405038/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:125092/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:471829/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1242001/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1439706/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2366974/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2441016/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2726344/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2808830/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:203652/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2864660/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1706627/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1657870/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1656814/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2995933/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1518068/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:3122100/40‑1 (MQ=25)
tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:3404661/40‑1 (MQ=25)
tGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATc > 1:425164/1‑64 (MQ=255)
tGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATc > 1:1541322/1‑64 (MQ=255)
tGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATc > 1:235628/1‑64 (MQ=255)
tGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATc > 1:1001996/1‑64 (MQ=255)
|
AGAAAATCTCGGTTCCGGATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACC > minE/2623566‑2623695
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
