Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,656,058 |
T→G |
38.8% |
A515A (GCT→GCG) |
glgP → |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,656,058 | 0 | T | G | 38.8%
| 41.9
/ 78.9
| 85 | A515A (GCT→GCG) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base T (38/14); new base G (27/6); total (65/20) |
| Fisher's exact test for biased strand distribution p-value = 4.37e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTTAGCCTGCTTAATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAATGTGG > minE/2656002‑2656125
|
cTTAGCCTGCTTAATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCg < 1:1117047/69‑1 (MQ=255)
cTTAGCCTGCTTAATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCg < 1:897246/69‑1 (MQ=255)
tAGCCTGCTTAATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGaa > 1:2640486/1‑69 (MQ=255)
tAGCCTGCTTAATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGaa > 1:2946854/1‑69 (MQ=255)
tAGCCTGCTTAATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGaa > 1:304815/1‑69 (MQ=255)
tAGCCTGCTTAATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGaa > 1:522236/1‑69 (MQ=255)
tAGCCTGCTTAATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGaa > 1:69371/1‑69 (MQ=255)
gCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCg > 1:2301432/1‑69 (MQ=255)
gCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCg > 1:3081208/1‑69 (MQ=255)
gCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCg < 1:3267131/69‑1 (MQ=255)
gCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCg < 1:1773999/69‑1 (MQ=255)
gCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCg < 1:1628184/69‑1 (MQ=255)
gCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCg > 1:413880/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTc < 1:2758789/68‑1 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:1077200/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:2150451/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:2097584/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:1154055/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:924233/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTc > 1:2839264/1‑68 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTc > 1:2945234/1‑68 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTc > 1:2926478/1‑68 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTc > 1:87175/1‑68 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:1704849/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:1862064/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:1143840/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:3329217/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:1031084/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:2151987/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:962803/1‑69 (MQ=255)
acaacaCTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCt > 1:750352/1‑69 (MQ=255)
aacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGa > 1:1778122/1‑45 (MQ=255)
aacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGa > 1:1392490/1‑45 (MQ=255)
aacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGa > 1:3380287/1‑45 (MQ=255)
aacaCTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGa > 1:674746/1‑45 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTggg > 1:3211693/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:93880/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:2875227/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:331139/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:792298/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:1055992/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:1111228/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:154464/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:2083761/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:2392014/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:133441/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:1350037/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTgg > 1:1419110/1‑68 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTg > 1:128445/1‑67 (MQ=255)
acacTGTGATTTCCCAATGGTTAATCACGCGGTACATCAGGCGAAGCTGGAGAACAAAAAGCGTCTg > 1:881966/1‑67 (MQ=255)
gtgATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCagag > 1:386900/1‑68 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtata > 1:1981686/1‑69 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtata > 1:1484349/1‑69 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtata > 1:2922185/1‑69 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtata > 1:2849053/1‑69 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtata > 1:1210292/1‑69 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtat < 1:1482276/68‑1 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtat < 1:38041/68‑1 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtat < 1:127145/68‑1 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtat < 1:2789049/68‑1 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGtat < 1:2899598/68‑1 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGta < 1:2838915/67‑1 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGta < 1:2081792/67‑1 (MQ=255)
aTTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGta < 1:1469446/67‑1 (MQ=255)
aaTGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCGGAGTATATCGCCca < 1:2481983/69‑1 (MQ=255)
aaTGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCGGAGTATATCGCCca < 1:695706/69‑1 (MQ=255)
aaTGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCGGAGTATATCGCCca < 1:1238273/69‑1 (MQ=255)
aaTGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCGGAGTATATCGCCca < 1:2799730/69‑1 (MQ=255)
aaTGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCGGAGTATATCGCCca < 1:2660311/69‑1 (MQ=255)
aaTGGTTAATCACGCGGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCGGAGTATATCGCCca < 1:1863381/69‑1 (MQ=255)
ttAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCcagcag > 1:3434232/1‑68 (MQ=255)
ttAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCcagcag > 1:3421938/1‑68 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGTGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:3279228/1‑69 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAATAAGCGTCTGGCAGAGTatat > 1:3038749/1‑52 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGc > 1:2962633/1‑36 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:2828716/1‑69 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:95883/1‑69 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:2321349/1‑69 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:704057/1‑69 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:2325916/1‑69 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:617160/1‑69 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:533136/1‑69 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:2307182/1‑69 (MQ=255)
aCGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAAtg > 1:2071701/1‑69 (MQ=255)
cTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAATgtgg > 1:1101506/1‑69 (MQ=255)
|
CTTAGCCTGCTTAATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAGCTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAATGTGG > minE/2656002‑2656125
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
