Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,661,032 |
G→A |
70.6% |
L181L (CTG→CTA) |
glpR → |
DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,661,032 | 0 | G | A | 70.6%
| 117.9
/ 56.7
| 122 | L181L (CTG→CTA) | glpR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (34/2); new base A (70/16); total (104/18) |
| Fisher's exact test for biased strand distribution p-value = 9.18e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 122 total aligned reads displayed.
AGTTCCGCCTTGATTTCGGCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAC > minE/2660974‑2661083
|
aGTTCCGCCTTGATTTCGGCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCg > 1:2849721/1‑69 (MQ=255)
tCCGCCTTGATTTCGGCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGAtt < 1:2335543/69‑1 (MQ=255)
ggCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:2060887/68‑1 (MQ=255)
ggCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:1930140/68‑1 (MQ=255)
ggCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:2109767/68‑1 (MQ=255)
ggCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:572931/68‑1 (MQ=255)
ggCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:28441/68‑1 (MQ=255)
ggCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:368265/68‑1 (MQ=255)
ggCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:2909474/68‑1 (MQ=255)
ggCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:3053076/68‑1 (MQ=255)
ggCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTACGATTACCACGAAGTTCg < 1:1732709/68‑1 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCAGCaaa > 1:1046216/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:751829/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:2905837/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:2855160/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1433201/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:3193268/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1870372/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1554667/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1067985/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:156746/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1586976/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1901301/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:263716/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1663747/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaa > 1:2959956/1‑68 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaa > 1:2415748/1‑68 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCaa > 1:2820800/1‑68 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTAACACGAAGTTCGCACCaaa > 1:1611605/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:857992/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:951201/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:2135769/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:2230075/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:581497/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:2526875/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:2768301/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:413827/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:306104/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1415591/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1406191/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1633914/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1225514/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1348151/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaaa > 1:1300978/1‑69 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaa > 1:1502799/1‑68 (MQ=255)
cTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCaa > 1:1739018/1‑68 (MQ=255)
tGGGGATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:1826351/61‑1 (MQ=255)
gggATAAGCGGCATCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:2508182/59‑1 (MQ=255)
aaGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCat < 1:3155744/69‑1 (MQ=255)
aTCGATAGCGCCGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCCCCAAACGCGCCATCATTgag > 1:1335504/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCCCGAAGTTCGCACCAAACGCGCCATCATTgag > 1:2445263/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCCCGAAGTTCGCACCAAACGCGCCATCATTgag > 1:271144/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:3059059/1‑62 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:3247445/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:1029183/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:3400016/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:3025125/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:548211/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:2949189/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:603330/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:716761/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:721953/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:2842751/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:1050736/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:192886/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:2765860/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:1845897/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:2165278/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:1794231/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:2608541/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTgag > 1:1667004/1‑69 (MQ=255)
aTCGATAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATCATTga > 1:200873/1‑68 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGaa > 1:1444915/1‑68 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAc > 1:1555583/1‑69 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAc > 1:929723/1‑69 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAc > 1:2605157/1‑69 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAc > 1:238851/1‑69 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAc > 1:236827/1‑69 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAc > 1:123679/1‑69 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAc > 1:1439528/1‑69 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAc > 1:1237874/1‑69 (MQ=255)
gATAGCGACGGCTCGCTGCTGGAGTTCGATGACCACGAAGTTCGCACCAAACGCGCCATTATTAAGAAc > 1:719037/1‑69 (MQ=255)
tAGCGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCg < 1:2094058/42‑1 (MQ=255)
cGACGGCTCGCTACTTGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:2206359/1‑54 (MQ=255)
cGACGGCTCGCTACTGGTGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:686685/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:1075072/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:793714/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:912938/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:2856019/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:227647/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:2680681/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:3007769/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:1353924/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:3387607/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:3264861/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:2497128/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:2501163/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:1344350/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCatca > 1:2558441/1‑54 (MQ=255)
cGACGGCTCGCTACTGGAGTTCGATTACCACGAAGTTCG‑ACCAAACGCGCCatca > 1:1187579/1‑53 (MQ=255)
|
AGTTCCGCCTTGATTTCGGCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCACGAAGTTCGCACCAAACGCGCCATTATTGAGAAC > minE/2660974‑2661083
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
