Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,758,108 |
T→C |
23.0% |
P76P (CCA→CCG) |
def ← |
peptide deformylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,758,108 | 0 | T | C | 23.0%
| 104.4
/ 25.4
| 82 | P76P (CCA→CCG) | def | peptide deformylase |
| Reads supporting (aligned to +/- strand): ref base T (19/44); new base C (8/11); total (27/55) |
| Fisher's exact test for biased strand distribution p-value = 4.06e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
CACGTTGTTCAGGGATCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAATGATACGTT > minE/2758041‑2758169
|
cACGTTGTTCAGGGATCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTg < 1:759532/69‑1 (MQ=255)
cAGGGATCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTg < 1:154929/60‑1 (MQ=255)
cAGGGATCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTg < 1:787003/60‑1 (MQ=255)
cAGGGATCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTg < 1:1625676/60‑1 (MQ=255)
cAGGGATCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTg < 1:475647/60‑1 (MQ=255)
cAGGGATCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTg < 1:3368054/60‑1 (MQ=255)
tCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCCGga > 1:341250/1‑56 (MQ=255)
tCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCCGga > 1:2001396/1‑56 (MQ=255)
tCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCCGga > 1:2002746/1‑56 (MQ=255)
tCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCCGga > 1:1389616/1‑56 (MQ=255)
tCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCCGga > 1:3430314/1‑56 (MQ=255)
tCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCCGga > 1:1311607/1‑56 (MQ=255)
tCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCCGga > 1:778537/1‑56 (MQ=255)
tCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCCGga > 1:797926/1‑56 (MQ=255)
cAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTaa < 1:1385408/56‑1 (MQ=255)
cAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTaa < 1:226478/56‑1 (MQ=255)
cAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTaa < 1:1127111/56‑1 (MQ=255)
cAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTc > 1:3213786/1‑68 (MQ=255)
gCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGt > 1:989928/1‑56 (MQ=255)
gCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCg > 1:2158494/1‑68 (MQ=255)
gCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa < 1:1456760/69‑1 (MQ=255)
gCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:1411746/1‑69 (MQ=255)
gCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa < 1:2344123/69‑1 (MQ=255)
gCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:578721/1‑69 (MQ=255)
gCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa < 1:1667389/69‑1 (MQ=255)
gCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa < 1:284736/69‑1 (MQ=255)
gCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:3125501/1‑69 (MQ=255)
gcTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:1679949/2‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:3424160/1‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:202930/1‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:2413017/1‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:1825955/1‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:1782225/1‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:3021341/1‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:1302319/1‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:1614874/1‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:980557/1‑68 (MQ=255)
ccTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGa > 1:1501820/1‑68 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACa < 1:1527709/66‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACa < 1:489026/66‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACa < 1:2514574/66‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACa < 1:128217/66‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACa < 1:2359567/66‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACa < 1:1098847/66‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACa < 1:1668105/66‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATc > 1:1918171/1‑68 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATc < 1:1883663/68‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATc < 1:144059/68‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATc < 1:652111/68‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATc < 1:1708214/68‑1 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATc > 1:340221/1‑68 (MQ=255)
tCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATc < 1:1352751/68‑1 (MQ=255)
tttttCTAAAAGCTCTTGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:1827712/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:1437778/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:1003216/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:907126/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:1075604/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:1636865/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:528528/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:388000/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:3390668/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:1429452/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:332521/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:1848124/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:2876720/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATAAATAACAAt < 1:899171/69‑1 (MQ=255)
tttttCTAAAAGCTCTGGATTGATTAACACTAGACGTTCGTCACGGTTTTCCGAAACATCAATAACAAt < 1:1351348/69‑1 (MQ=255)
ttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAATGa < 1:3416729/68‑1 (MQ=255)
ttCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAATGa < 1:1232390/68‑1 (MQ=255)
aGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAATGATACGtt < 1:3355842/67‑1 (MQ=255)
aGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAATGATACGtt < 1:1363977/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:1748522/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:1719936/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:68191/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:2637298/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:418886/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:1849190/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:1324229/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:2306324/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:1533249/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGTCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:1719093/67‑1 (MQ=255)
aGCTCCGGATTGATTAACACCAGCCGTTCGGCACGGTTTTCCGAAACATCAATTACAATGATACGtt < 1:1667239/67‑1 (MQ=255)
|
CACGTTGTTCAGGGATCGACAGGCAACCTTCTTCAATGCCTGTTTCGCCGCTTTTTTCTAAAAGCTCTGGATTGATTAACACTAGCCGTTCGTCACGGTTTTCCGAAACATCAATAACAATGATACGTT > minE/2758041‑2758169
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
