Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,780,693 |
G→A |
26.8% |
A556A (GCG→GCA) |
metH → |
homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,780,693 | 0 | G | A | 26.8%
| 88.3
/ 28.9
| 78 | A556A (GCG→GCA) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (46/11); new base A (17/4); total (63/15) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAACAACTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATC > minE/2780630‑2780745
|
caacaaCTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGAt > 1:578325/1‑69 (MQ=255)
caacaaCTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGAt > 1:293516/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGAttcc > 1:1532143/1‑67 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:2275530/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:1630963/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:1578533/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:3146101/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:1987524/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:526380/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:570884/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:2004006/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:1090750/1‑69 (MQ=255)
caatTACGCACAGGACTTTATCGGCGCGTATGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTc > 1:1145402/1‑69 (MQ=255)
acTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcg > 1:1955838/1‑69 (MQ=255)
acTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcg > 1:2106008/1‑69 (MQ=255)
acTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcg > 1:2333671/1‑69 (MQ=255)
acTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcg > 1:1008746/1‑69 (MQ=255)
acTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcg > 1:1879428/1‑69 (MQ=255)
acTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcg > 1:2988190/1‑69 (MQ=255)
acTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcg > 1:1482367/1‑69 (MQ=255)
aCGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTgcta > 1:2318153/1‑59 (MQ=255)
aCGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGAtt > 1:167108/1‑62 (MQ=255)
aCGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGAtt > 1:2217370/1‑62 (MQ=255)
aCGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGAtt > 1:2625434/1‑62 (MQ=255)
aCGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGAtt > 1:3424644/1‑62 (MQ=255)
cgcATGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcggcgg < 1:1706355/67‑1 (MQ=255)
cgcAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcggcgg < 1:2877195/67‑1 (MQ=255)
cgcAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcggcgg < 1:1147613/67‑1 (MQ=255)
cgcAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcggcgg < 1:3168474/67‑1 (MQ=255)
cgcAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcggcgg < 1:646857/67‑1 (MQ=255)
cgcAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGgcggcg < 1:1993706/69‑1 (MQ=255)
cgcAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGgcggcg < 1:1215262/69‑1 (MQ=255)
cgcAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGgcggcg < 1:306019/69‑1 (MQ=255)
gcAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGgcggcg < 1:2142107/68‑1 (MQ=255)
aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:2864585/1‑69 (MQ=255)
aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:2511895/1‑69 (MQ=255)
aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:2342857/1‑69 (MQ=255)
aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:1044215/1‑69 (MQ=255)
aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:1076592/1‑69 (MQ=255)
aCTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:1318247/1‑69 (MQ=255)
cTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTa > 1:633209/1‑68 (MQ=255)
gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCcggcgg > 1:1524191/1‑50 (MQ=255)
gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:3140688/1‑69 (MQ=255)
gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:3175312/1‑69 (MQ=255)
gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:2073586/1‑69 (MQ=255)
gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:1694831/1‑69 (MQ=255)
gcgTGTGAAGACATCAAACGTGAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGtgtcgttc > 1:370677/1‑69 (MQ=255)
gtgtGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGgcggcg < 1:2772742/50‑1 (MQ=255)
aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGGGTATCTAACGTTTCTTTCTCGTTcc > 1:224672/1‑69 (MQ=255)
aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:3426869/1‑69 (MQ=255)
aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:1930512/1‑69 (MQ=255)
aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:2925311/1‑69 (MQ=255)
aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:19044/1‑69 (MQ=255)
aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:2654285/1‑69 (MQ=255)
aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:259632/1‑69 (MQ=255)
aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:1262110/1‑69 (MQ=255)
aaGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTcc > 1:1649691/1‑69 (MQ=255)
tCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCa < 1:554944/69‑1 (MQ=255)
aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCt > 1:2839176/1‑42 (MQ=255)
aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAAc > 1:2115145/1‑69 (MQ=255)
aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAAc > 1:1553536/1‑69 (MQ=255)
aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAAc > 1:2388940/1‑69 (MQ=255)
aaaCGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAAc > 1:3007848/1‑69 (MQ=255)
gtgAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGTGTCGTTCTCGTTTCGTGGCAACGATc < 1:646523/67‑1 (MQ=255)
gtgAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGTGTCGTTCTCGTTTCGTGGCAACGATc < 1:1229258/67‑1 (MQ=255)
gtgAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGTGTCGTTCTCGTTTCGTGGCAACGATc < 1:517945/67‑1 (MQ=255)
gtgAACTGCCGCACGCACTGATTTCCGGCGGCGTTTCTAACGTGTCGTTCTCGTTTCGTGGCAACGATc < 1:2103134/67‑1 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:699103/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:2383541/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:1123011/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:2461658/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:376749/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:268059/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:198392/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:3299863/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:3255811/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:3253766/1‑59 (MQ=255)
gCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACg > 1:3028480/1‑59 (MQ=255)
|
CAACAACTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCGGCGGCGTATCTAACGTTTCTTTCTCGTTCCGTGGCAACGATC > minE/2780630‑2780745
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
